Apparatus and Methods for Laser Doppler Electrophoresis

Smith, Barton A.; Ware, B. R.

doi:10.1007/978-1-4615-6731-8_2

Cited by 38 publications

(9 citation statements)

References 33 publications

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“…It is a design difficulty that it is not easy to identify and locate a stationary layer in small capillary (0.7 mm); thus, in all cases a coated capillary was used (15). The presence of the methylcellulose inhibits electro-osmosis by locating the effective plane of shear within a high viscosity matrix.…”

Section: Laser Doppler Velocimetry (Ldv)mentioning

confidence: 99%

Factors Involved in the Cytotoxicity of UICC Amosite towards Macrophages

Davies¹,

Griffiths²,

Preece³

1983

Environmental Health Perspectives

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Section: Laser Doppler Velocimetry (Ldv)mentioning

confidence: 99%

Factors Involved in the Cytotoxicity of UICC Amosite towards Macrophages

Davies¹,

Griffiths²,

Preece³

1983

Environmental Health Perspectives

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“…The best results and practical elimination of the electro-endosmosis effect have been obtained by a double-coating procedure described by Smith & Ware (1978). This procedure takes advantage of the reduction of the c-potential of the wall and of the increase of the resistance to flow in the diffuse double layer by methylcellulose.…”

Section: Electro-endosmosismentioning

confidence: 99%

Cell electrophoresis for diagnostic purposes. II. Critical evaluation of conventional cytopherometry

Hoffmann¹,

Kaufmann²,

Steiner³

et al. 1981

Br J Cancer

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Determination of the electrophoretic mobility of test cells has been widely used in an attempt to detect so-called lymphokines in a laboratory test for cancer, but operational difficulties are inherent in conventional cytopherometers. This study therefore investigates the technical and operational aspects of cell electrophoresis, using the Zeiss cytopherometer; e.g. influence of electro-osmosis, focus uncertainty, movement due to convection and other sources of error. Implications and possible improvements in the test are discussed.

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“…The electrophoretic light-scattering technique and apparatus have been previously described (6,19). In the present work, either a helium-neon laser (632.8 nm wavelength) or an argon-ion laser (operating at 514.5 nm) was used as the coherent light source.…”

Section: Electrophoretic Light Scattering (Laser Doppler Electrophorementioning

confidence: 99%

“…The widths of such spectra for particles in the size range of subcellular organelles are a function of both the electrophoretic mobility heterogeneity and the diffusive motion of the particles in the sample ( 19). Extrapolations of plots of half-width at half-height versus electric field strength to zero applied field yield a rough estimate of the weight-average diffusion coefficient of the particles in the sample.…”

Section: Electrophoretic Mobilities Ofsynaptic Vesiclesmentioning

confidence: 99%

Electrokinetic properties of synaptic vesicles and synaptosomal membranes

Siegel¹,

Ware²

1980

Biophysical Journal

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Using the technique of electrophoretic light scattering, we have measured the electrophoretic mobilities of synaptic vesicles and synaptosomal plasma membranes isolated from guinea-pig cerebral cortex. The electrophoretic mobility of synaptic vesicles is slightly greater than that of synaptosomal plasma membranes. Ca+2 and Mg+2 reduced the mobility of both species to the same extent at physiologically relevant concentrations (0-1 mM) and near-physiologic ionic strength. The extent of the reduction was not large (approximately 6% for synaptic vesicles in the presence of 100 mM KCl) at 1 mM divalent cation concentrations. At concentrations of approximately 2 mM and higher, Ca+2 reduced the mobility of synaptic vesicles more than did Mg/2. A similar but much smaller effect was observed in the case of synaptosomal plasma membranes. The addition of 1 mM Mg+2-ATP had no effect upon synaptic vesicle mobility either in the presence or absence of the ionophores nigericin or valinomycin. These data, together with earlier work (Siegel et al., 1978, Biophys. J. 22:341-346), demonstrate that substantial reduction of the average electrostatic surface charge density is not the most important role of divalent cations in promoting close approach of secretory granules and secretory cell membranes, and that it is certainly not the Ca+2-specific step in exocytosis.

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Apparatus and Methods for Laser Doppler Electrophoresis

Cited by 38 publications

References 33 publications

Factors Involved in the Cytotoxicity of UICC Amosite towards Macrophages

Factors Involved in the Cytotoxicity of UICC Amosite towards Macrophages

Cell electrophoresis for diagnostic purposes. II. Critical evaluation of conventional cytopherometry

Electrokinetic properties of synaptic vesicles and synaptosomal membranes

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