Apparatus for the Observation of Infrared Streaming Dichroism of Polymer Solutions

Abstract

A method and apparatus have been developed for the quantitative observation of infrared streaming dichroism of solutions of macromolecules, The design, construction, and operation of the apparatus are described in detail. The apparatus consists of a modified standard infrared liquid c~ll through which the solution is pumped by means of a pair of hypodermic syringes which are driven in opposition by a pair of cams that provide a constant rate of displacement. The resulting flow is constant except for a reversal in direction at the end of each stroke. The maximum value of the gradient in the cell may be calculated by means of a simple formula. The total volume of the pump and cell is 2,2 cc so that small samples may be measured, Several types of materials have been measured and a typical result is shown,

A proposal for a dynamic h‐type index

“…Elkan Blout played a very important role in the experimental studies of the helix–coil transition by developing methods to synthesize high‐molecular‐weight poly‐α‐amino acids,2, 10‐16 to study their properties, including the helix–coil transition,17‐21 with optical rotation,22‐29 circular dichroism,30, 31 and infrared spectroscopy,32‐38 and by demonstrating the biological properties of some of these homopolymers, e.g., their antigenicity 39. He also developed synthetic methods to study polyproline and models of the triple helix of collagen,31, 40‐48 including the triple helix‐to‐coil transition,49 and demonstrated the presence of cis peptide bonds preceding proline 50.…”