Apparent dominance of the G1–G3 genetic cluster of Echinococcus granulosus strains in the central inland region of Portugal

Beato, Sílvia; Parreira, Ricardo; Calado, Maria Manuela Palmeiro; Grácio, Maria Amélia Afonso

doi:10.1016/j.parint.2010.08.004

Cited by 26 publications

(21 citation statements)

References 13 publications

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“…G1 is also the common genotype in different hosts in Ethiopia [31], Tunisia [39], Palestine [41], Iran [42], India [43], China [44] and Mongolia [45]. Echinococcus granulosus s.s. (G1- G3 complex) is also the major genotype in humans, cattle, sheep and goats in many European and Latin American countries [27,46,47]. …”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Echinococcus granulosus Sensu Lato from Farm Animals in Egypt

et al. 2015

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Little is known on the diversity and public health significance of Echinococcus species in livestock in Egypt. In this study, 37 individual hydatid cysts were collected from dromedary camels (n=28), sheep (n=7) and buffalos (n=2). DNA was extracted from protoscoleces/germinal layer of individual cysts and amplified by PCR targeting nuclear (actin II) and mitochondrial (COX1 and NAD1) genes. Direct sequencing of amplicons indicated the presence of Echinococcus canadenesis (G6 genotype) in 26 of 28 camel cysts, 3 of 7 sheep cysts and the 2 buffalo derived cysts. In contrast, Echinococcus granulosus sensu stricto (G1 genotype) was detected in one cyst from a camel and 4 of 7 cysts from sheep, whereas Echinococcus ortleppi (G5 genotype) was detected in one cyst from a camel. This is the first identification of E. ortleppi in Egypt.

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Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Echinococcus granulosus Sensu Lato from Farm Animals in Egypt

et al. 2015

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“…granulosus s.s. These two genotypes have already been described in Europe in sheep and cattle, notably in Italy (Busi et al 2007; Casulli et al 2008), Romania (Bart et al 2006), Portugal (Beato et al 2010) and Turkey (Vural et al 2008). The genotype G1 is mainly responsible for infection in humans, but genotypes G2 and G3 are known to be infectious as well (Busi et al 2007; Eckert and Thompson 1997; Maillard et al 2007).…”

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confidence: 87%

Prevalence survey and first molecular characterization of Echinococcus granulosus in France

et al. 2012

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Human cystic echinococcosis (hydatid disease) caused by the Echinococcus granulosus tapeworm continues to be a substantial cause of morbidity and mortality in many parts of the world. France is still considered as endemic area, but the current infestation by E. granulosus of intermediate hosts in France remains currently unknown due to the absence of official data reporting for the last 20 years. A 1-year prevalence survey was conducted in the 24 slaughterhouses of ten departments of the South of France. We demonstrate that the E. granulosus parasite is still currently present at low prevalence at slaughterhouses in the study area (4 cases for 100,000 sheep and 3 cases for 100,000 cattle). In addition, we assess the presence of genotype G1 in infected animals and identify for the first time in France genotypes G2 and G3 of E. granulosus sensu stricto.

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“…The PCRs allowed the discrimination between all Echinococcus species and genotypes included in this study, and phylogenetic trees constructed using either rrnS, rrnL or concatenated sequence data produced similar topologies, in particular the close relationships between E. granulosus s.s. or E. canadensis genotype members [5]. Since the discrimination between G1 and G3 has been a controversial issue and has largely relied on the use of mitochondrial gene markers [26], many studies have allowed only E. granulosus s.s. to be defined, without a discrimination among genotypes G1, G2 and G3 [50,51]. The PCRs should also be tested on DNA isolated directly from faeces from Echinococcus-infected definitive hosts [54], or even from environmental samples, such as soil or water [55].…”

Section: Discussionmentioning

confidence: 99%

“…To date, molecular phylogenetic analyses have relied on DNA sequence data obtained from partial single loci (ranging from ~250 to 500 bp in length), particularly from mitochondrial DNA loci, such as cytochrome c oxidase subunit 1 (cox1) [17,22,23], NADH dehydrogenase subunit (nad1) [22][23][24][25], ATP synthase subunit (atp6) [26] and the small subunit (SSU) of the rRNA gene [27,28]. However, these relatively short sequences have insufficient discriminatory power to resolve fine scale taxonomic peculiarities (e.g.…”

Section: Introductionmentioning

confidence: 99%

A dual PCR-based sequencing approach for the identification and discrimination of Echinococcus and Taenia taxa

Boubaker

Marinova

Gori

et al. 2016

Molecular and Cellular Probes

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Reliable and rapid molecular tools for the genetic identification and differentiation of Echinococcus species and/or genotypes are crucial for studying spatial and temporal transmission dynamics. Here, we describe a novel dual PCR targeting regions in the small (rrnS) and large (rrnL) subunits of mitochondrial ribosomal RNA (rRNA) genes, which enables (i) the specific identification of species and genotypes of Echinococcus (rrnS + L-PCR) and/or (ii) the identification of a range of taeniid cestodes, including different species of Echinococcus, Taenia and some others (17 species of diphyllidean helminths). This dual PCR approach was highly sensitive, with an analytical detection limit of 1 pg for genomic DNA of Echinococcus. Using concatenated sequence data derived from the two gene markers (1225 bp), we identified five unique and geographically informative single nucleotide polymorphisms (SNPs) that allowed genotypes (G1 and G3) of Echinococcus granulosus sensu stricto to be distinguished, and 25 SNPs that allowed differentiation within Echinococcus canadensis (G6/7/8/10). In conclusion, we propose that this dual PCR-based sequencing approach can be used for molecular epidemiological studies of Echinococcus and other taeniid cestodes.

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Apparent dominance of the G1–G3 genetic cluster of Echinococcus granulosus strains in the central inland region of Portugal

Cited by 26 publications

References 13 publications

Molecular Characterization of Echinococcus granulosus Sensu Lato from Farm Animals in Egypt

Molecular Characterization of Echinococcus granulosus Sensu Lato from Farm Animals in Egypt

Prevalence survey and first molecular characterization of Echinococcus granulosus in France

A dual PCR-based sequencing approach for the identification and discrimination of Echinococcus and Taenia taxa

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