Apparent Fusion of the TOL Plasmid with the R91 Drug Resistance Plasmid in Pseudomonas aeruginosa

White, GP; Dunn, NW

doi:10.1071/bi9770345

Cited by 30 publications

(16 citation statements)

References 12 publications

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“…The concatamerization of multiple drug resistance genes on a single episomal element is thought to occur through a stepwise fusion of multiple resistance plasmids. Similar fusion events have been found to occur for of R factors and TOL plasmids, the latter of which carry genes essential for the catabolism of small aromatic compounds present in soil (336). The second mechanism by which a single genetic event can result in resistance to multiple drugs is the acquisition of genes encoding drug efflux pumps (179).…”

Section: Mechanisms Of Antibiotic Resistancementioning

confidence: 88%

Lung Infections Associated with Cystic Fibrosis

2002

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While originally characterized as a collection of related syndromes, cystic fibrosis (CF) is now recognized as a single disease whose diverse symptoms stem from the wide tissue distribution of the gene product that is defective in CF, the ion channel and regulator, cystic fibrosis transmembrane conductance regulator (CFTR). Defective CFTR protein impacts the function of the pancreas and alters the consistency of mucosal secretions. The latter of these effects probably plays an important role in the defective resistance of CF patients to many pathogens. As the modalities of CF research have changed over the decades from empirical histological studies to include biophysical measurements of CFTR function, the clinical management of this disease has similarly evolved to effectively address the ever-changing spectrum of CF-related infectious diseases. These factors have led to the successful management of many CF-related infections with the notable exception of chronic lung infection with the gram-negative bacterium Pseudomonas aeruginosa. The virulence of P. aeruginosa stems from multiple bacterial attributes, including antibiotic resistance, the ability to utilize quorum-sensing signals to form biofilms, the destructive potential of a multitude of its microbial toxins, and the ability to acquire a mucoid phenotype, which renders this microbe resistant to both the innate and acquired immunologic defenses of the host

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Section: Mechanisms Of Antibiotic Resistancementioning

confidence: 88%

Lung Infections Associated with Cystic Fibrosis

2002

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“…--2-2-0 become integrated into an R plasmid without any detectable chromosome-linked intermediate (22,33).…”

Section: Methodsmentioning

confidence: 99%

Naturally occurring TOL plasmids in Pseudomonas strains carry either two homologous or two nonhomologous catechol 2,3-oxygenase genes

Chatfield¹,

Williams²

1986

J Bacteriol

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Structural genes for catechol 2,3-oxygenase (C230) were cloned from the TOL plasmids pWW5, pWW14, pWW74, pWW84, and pWW88 isolated fro*n Pseudomonas strains of diverse geographical origins. Each pKT230-based C230+ recombinant plasmid carried a 2.05-kilobase XhoI insert which showed strong homology in Southern hybridizations with the xyLE gene from the archetype TOL plasmid pWWO. Fragments were mapped for restriction endonuclease sites and were classified into two closely related groups on the basis of restriction maps. C230 structural genes were located on cloned fragments by a combination of subcloning and site-specific mutagenesis. All five TOL plasmids examined yielded clones whose maps differed from that of xyLE of pWWO by only a single XbaI site, but in addition plasmids pWW5, pWW74, and pWW88 carried a second, homologous C230 gene with seven further restriction site differences. The remaining plasmids, pWW14 and pWW84, carried a second nonhomologous C230 gene related to the second C230 gene (C2301) of TOL plasmid pWW15 described previously (H. Keil, M. R. Lebens, and P. A. Williams, J. Bacteriol. 163:248&255, 1985). Thus, each naturally occurring TOL plasmid in this study appears to carry genes for two meta cleavage dioxygenases.The degradation of toluene and some substituted toluenes via the meta pathway by Pseudomonas spp. is often plasmid mediated (7,15,19,26,34,35,39). TOL plasmids from different strains are diverse with regard to molecular sizes, fragmentation patterns by restriction endonucleases, selftransmissibility, rate of dissimilation ofp-methyl-substituted substrates, and formation of different classes of deletion mutants after growth on benzoate (7,17,27,35,37,38).Although the archetype TOL plasmid pWWO (34) has a single xylE gene coding for the ring cleavage dioxygenase catechol 2,3-oxygenase (C230), it was recently demonstrated in this laboratory that the 250-kilobase (kb) plasmid pWW15 carries two nonhomologous genes which specify the apparently unrelated enzymes C2301 and C2301I (16). To investigate the evolutionary relationship between the catabolic genes on different TOL plasmids, we cloned xylE genes from five TOL plasmids found in Pseudomonas strains isolated from soil samples of diverse geographical origins. Like pWW15, two of the plasmids reported here also carried two nonhomologous C230 genes. Unexpectedly, the other plasmids in this study each possessed two closely related C230 genes which exhibited strong homology with each other and with the xylE gene of pWWO. MATERIALS AND METHODSBacterial strains and plasmids. Pseudomonas strains and plasmids used in this study are described in Table 1. Strains MT74, MT84, and MT88 were isolated by selective enrichment on m-toluate minimal medium during this study; MT5 and MT14-26 have been described previously (7,26,35).

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“…Several workers have reported the isolation of hybrid plasmids between the TOL plasmid pWWO and various R plasmids (4,16,19,20,32), and it has been suggested that the TOL genes may be carried on a transposonlike element. However, Lehrbach et al (13) showed by restriction endonuclease analysis that the amount of TOL DNA present in the hybrid plasmids varied from 56 to 104 kilobases (kb), and thus it is clear that transposition of a unique DNA segment from the TOL plasmid cannot be responsible for hybrid formation.…”

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confidence: 99%

Chromosomal location of TOL plasmid DNA in Pseudomonas putida

Sinclair¹,

Maxwell²,

Lyon³

et al. 1986

J Bacteriol

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The soil isolate Pseudomonas putida MW1000 can grow on toluene and other hydrocarbons; in this respect it is similar to strains of Pseudomonas which carry the TOL plasmid. By conjugation experiments, the genes conferring these growth abilities have been shown to be located on the bacterial chromosome, linked to vil and catB. A 56-kilobase segment of the bacterial chromosome of MW strains carrying the TOL genes can transpose to the IncP-1 plasmid R18-18. Physical analysis of these TOL R18-18 hybrids has shown that the TOL segment is almost identical to the same region found in the TOL plasmid pWWO.

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Apparent Fusion of the TOL Plasmid with the R91 Drug Resistance Plasmid in Pseudomonas aeruginosa

Cited by 30 publications

References 12 publications

Lung Infections Associated with Cystic Fibrosis

Lung Infections Associated with Cystic Fibrosis

Naturally occurring TOL plasmids in Pseudomonas strains carry either two homologous or two nonhomologous catechol 2,3-oxygenase genes

Chromosomal location of TOL plasmid DNA in Pseudomonas putida

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