Appetite is correlated with octopamine and hemolymph sugar levels in forager honeybees

Mayack, Christopher; Phalen, Nicole; Carmichael, Kathleen; White, Helen K.; Hirche, Frank; Wang, Ying; Stangl, Gabriele I.; Amdam, Gro V.

doi:10.1007/s00359-019-01352-2

Cited by 21 publications

(25 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They also varied with age, being higher in older foragers compared to nurses 63 , 65 . Yet, in this work, there was no correlation between increased sucrose responsiveness and AKH levels, which remained constant throughout the ages tested and the feeding regime experienced by the bees 64 . Here we used a different strategy that consisted in manipulating AKH levels and studying the effect of this manipulation on partially fed bees.…”

Section: Discussioncontrasting

confidence: 61%

“…A recent study focused on AKH in honey bees and on its possible variation throughout different ages and feeding states 64 . As in the present work, fed and starved bees were subjected to the standard test of sucrose responsiveness 64 in which PER to increasing sugar concentrations was quantified. As in our experiments, PER levels were higher in starved than in fed bees.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Adipokinetic hormone (AKH), energy budget and their effect on feeding and gustatory processes of foraging honey bees

Sanchez

Muñoz

Chen

et al. 2021

Sci Rep

View full text Add to dashboard Cite

The adipokinetic hormone (AKH) of insects is considered an equivalent of the mammalian hormone glucagon as it induces fast mobilization of carbohydrates and lipids from the fat body upon starvation. Yet, in foraging honey bees, which lack fat body storage for carbohydrates, it was suggested that AKH may have lost its original function. Here we manipulated the energy budget of bee foragers to determine the effect of AKH on appetitive responses. As AKH participates in a cascade leading to acceptance of unpalatable substances in starved Drosophila, we also assessed its effect on foragers presented with sucrose solution spiked with salicin. Starved and partially-fed bees were topically exposed with different doses of AKH to determine if this hormone modifies food ingestion and sucrose responsiveness. We found a significant effect of the energy budget (i.e. starved vs. partially-fed) on the decision to ingest or respond to both pure sucrose solution and sucrose solution spiked with salicin, but no effect of AKH per se. These results are consistent with a loss of function of AKH in honey bee foragers, in accordance with a social life that implies storing energy resources in the hive, in amounts that exceed individual needs.

show abstract

Section: Discussioncontrasting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Adipokinetic hormone (AKH), energy budget and their effect on feeding and gustatory processes of foraging honey bees

Sanchez

Muñoz

Chen

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Not surprisingly, as the bee ages and transitions from one behavioral task to the next, there is also an increase in metabolism and appetite levels as well [29][30][31][32]. As the bee ages, there is also a corresponding increase in octopamine, dopamine, and serotonin levels, but a lowering in tyramine levels in the brain [33,34]. When transitioning from an in-hive to a forager bee, there is a reduction in fat stores to increase flight efficiency [35], an increase in vitellogenin (Vg) [36] and juvenile hormone (JH) [37,38], and an increased reliance upon trehalose sugar levels to fuel flight when foraging [8].…”

Section: Introductionmentioning

confidence: 99%

Age-Dependent Honey Bee Appetite Regulation Is Mediated by Trehalose and Octopamine Baseline Levels

Akülkü

Ghanem

Filiztekin

et al. 2021

Insects

Self Cite

View full text Add to dashboard Cite

There are multiple feedback mechanisms involved in appetite regulation, which is an integral part of maintaining energetic homeostasis. Older forager honey bees, in comparison to newly emerged bees and nurse bees, are known to have highly fluctuating hemolymph trehalose levels, higher appetite changes due to starvation, and higher octopamine levels in the brain. What remains unknown is if the hemolymph trehalose and octopamine levels interact with one another and how this varies as the bee ages. We manipulated trehalose and octopamine levels across age using physiological injections and found that nurse and forager bees increase their appetite levels due to increased octopamine levels in the brain. This is further enhanced by lower trehalose levels in the hemolymph. Moreover, nurse bees with high octopamine levels in the brain and low trehalose levels had the same appetite levels as untreated forager bees. Our findings suggest that the naturally higher levels of octopamine as the bee ages may result in higher sensitivity to fluctuating trehalose levels in the hemolymph that results in a more direct way of assessing the energetic state of the individual. Consequently, forager bees have a mechanism for more precise regulation of appetite in comparison to newly emerged and nurse bees.

show abstract

“…In the context of stress, octopamine liberates stored nutrients to fuel the organism's behavioral response (Adamo et al, 1995;Arrese and Soulages, 2010;Fields and Woodring, 1991;Gage et al, 2018;Harris and Woodring, 1992;Hiripi et al, 1994;Johnson, 2017;Li et al, 2016;Orchard et al, 1982;Tao et al, 2016;Woodring et al, 1993). In honey bees, octopamine also induces the nurse to forager transition (Barron and Robinson, 2005;Barron et al, 2002;Mayack et al, 2019;Scheiner et al, 2002;Schulz et al, 2002a). Bees transitioning from nurse to forager typically show a reduction in abdominal lipid stores and HG size (Crailsheim and Stolberg, 1989;.…”

Section: Discussionmentioning

confidence: 99%

“…In honey bees, octopamine is a key element of the nurse-toforager transition because it induces foraging and increases forager sensitivity to foraging-related hive cues (Barron and Robinson, 2005; Barron et al, 2002;Mayack et al, 2019;Schulz et al, 2002a).…”

Section: Introductionmentioning

confidence: 99%

Octopamine mobilizes lipids from honey bee (Apis mellifera) hypopharyngeal glands

Corby‐Harris

Deeter

Snyder

et al. 2020

Journal of Experimental Biology

View full text Add to dashboard Cite

Recent widespread honey bee (Apis mellifera) colony loss is attributed to a variety of stressors, including parasites, pathogens, pesticides and poor nutrition. In principle, we can reduce stressinduced declines in colony health by either removing the stressor or increasing the bees' tolerance to the stressor. This latter option requires a better understanding than we currently have of how honey bees respond to stress. Here, we investigated how octopamine, a stress-induced hormone that mediates invertebrate physiology and behavior, influences the health of young nurse-aged bees. Specifically, we asked whether octopamine induces abdominal lipid and hypopharyngeal gland (HG) degradation, two physiological traits of stressed nurse bees. Nurse-aged workers were treated topically with octopamine and their abdominal lipid content, HG size and HG autophagic gene expression were measured. Hemolymph lipid titer was measured to determine whether tissue degradation was associated with the release of nutrients from these tissues into the hemolymph. The HGs of octopamine-treated bees were smaller than control bees and had higher levels of HG autophagy gene expression. Octopamine-treated bees also had higher levels of hemolymph lipid compared with control bees. Abdominal lipids did not change in response to octopamine. Our findings support the hypothesis that the HGs are a rich source of stored energy that can be mobilized during periods of stress.

show abstract

Appetite is correlated with octopamine and hemolymph sugar levels in forager honeybees

Cited by 21 publications

References 63 publications

Adipokinetic hormone (AKH), energy budget and their effect on feeding and gustatory processes of foraging honey bees

Adipokinetic hormone (AKH), energy budget and their effect on feeding and gustatory processes of foraging honey bees

Age-Dependent Honey Bee Appetite Regulation Is Mediated by Trehalose and Octopamine Baseline Levels

Octopamine mobilizes lipids from honey bee (Apis mellifera) hypopharyngeal glands

Contact Info

Product

Resources

About