Apple F-Box Protein MdMAX2 Regulates Plant Photomorphogenesis and Stress Response

The basic leucine zipper (bZIP) transcription factor HY5 plays a multifaceted role in plant growth and development. Here the apple MdHY5 gene was cloned based on its homology with Arabidopsis HY5. Expression analysis demonstrated that MdHY5 transcription was induced by light and abscisic acid treatments. Electrophoretic mobility shift assays and transient expression assays subsequently showed that MdHY5 positively regulated both its own transcription and that of MdMYB10 by binding to E-box and G-box motifs, respectively. Furthermore, we obtained transgenic apple calli that overexpressed the MdHY5 gene, and apple calli coloration assays showed that MdHY5 promoted anthocyanin accumulation by regulating expression of the MdMYB10 gene and downstream anthocyanin biosynthesis genes. In addition, the transcript levels of a series of nitrate reductase genes and nitrate uptake genes in both wild-type and transgenic apple calli were detected. In association with increased nitrate reductase activities and nitrate contents, the results indicated that MdHY5 might be an important regulator in nutrient assimilation. Taken together, these results indicate that MdHY5 plays a vital role in anthocyanin accumulation and nitrate assimilation in apple.

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“…43 Differences were considered statistically significant when P <0.05 and P <0.01. The results were analyzed in triplicate.…”

Section: Methodsmentioning

confidence: 99%

The bZIP transcription factor MdHY5 regulates anthocyanin accumulation and nitrate assimilation in apple

An¹,

Qu²,

Yao³

et al. 2017

Hortic Res

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257

183

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“…But the detailed molecular mechanism awaits to be elucidated [60]. A number of studies also have indicated that MAX2 contributes to plant responses to both biotic and abiotic stresses through modulation of the ABA signaling pathway and that MAX2 gene expression can be influenced by ABA treatment [42, 75, 88]. Bu et al showed that MAX2 expression was suppressed to about 50% after Arabidopsis seedlings were treated with 50 μM ABA for 6 h [42].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide analysis and characterization of F-box gene family in Gossypium hirsutum L

Zhang¹,

Tian²,

Li³

et al. 2019

BMC Genomics

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BackgroundF-box proteins are substrate-recognition components of the Skp1-Rbx1-Cul1-F-box protein (SCF) ubiquitin ligases. By selectively targeting the key regulatory proteins or enzymes for ubiquitination and 26S proteasome mediated degradation, F-box proteins play diverse roles in plant growth/development and in the responses of plants to both environmental and endogenous signals. Studies of F-box proteins from the model plant Arabidopsis and from many additional plant species have demonstrated that they belong to a super gene family, and function across almost all aspects of the plant life cycle. However, systematic exploration of F-box family genes in the important fiber crop cotton (Gossypium hirsutum) has not been previously performed. The genome-wide analysis of the cotton F-box gene family is now possible thanks to the completion of several cotton genome sequencing projects.ResultsIn current study, we first conducted a genome-wide investigation of cotton F-box family genes by reference to the published F-box protein sequences from other plant species. 592 F-box protein encoding genes were identified in the Gossypium hirsutume acc.TM-1 genome and, subsequently, we were able to present their gene structures, chromosomal locations, syntenic relationships with their parent species. In addition, duplication modes analysis showed that cotton F-box genes were distributed to 26 chromosomes, with the maximum number of genes being detected on chromosome 5. Although the WGD (whole-genome duplication) mode seems play a dominant role during cotton F-box gene expansion process, other duplication modes including TD (tandem duplication), PD (proximal duplication), and TRD (transposed duplication) also contribute significantly to the evolutionary expansion of cotton F-box genes. Collectively, these bioinformatic analysis suggest possible evolutionary forces underlying F-box gene diversification. Additionally, we also conducted analyses of gene ontology, and expression profiles in silico, allowing identification of F-box gene members potentially involved in hormone signal transduction.ConclusionThe results of this study provide first insights into the Gossypium hirsutum F-box gene family, which lays the foundation for future studies of functionality, particularly those involving F-box protein family members that play a role in hormone signal transduction.

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“…Apple ( Malus domestica ) is an important commercial fruit crop, and its production is highly affected by environmental factors, especially light [ 29 , 30 ]. PIFs play a central role in adapting plants to different light conditions; however, identification and functional analysis of PIFs in apples have not been systematically investigated, except MdPIF1 [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of Phytochrome-Interacting Factor Family Members and Functional Analysis of MdPIF4 in Malus domestica

Zheng

Wang

Yang

et al. 2020

IJMS

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Phytochrome-interacting factors (PIFs), members of the basic helix-loop-helix transcription factor family that have been extensively investigated in Arabidopsis thaliana, play essential roles in plant growth and development. However, PIF members have not been systematically investigated in apples, a worldwide perennial woody crop of economic importance. Here, seven PIF genes were identified from the Malus × domestica reference genome. Chromosomal locations, gene structures, and phylogenetic relationships of these members were analyzed. Analysis of cis-acting elements in promoter regions of MdPIF genes indicated that various elements were related to light, abiotic stress, and plant hormone responsiveness. Subsequently, subcellular localization and transcriptional activity analysis revealed that MdPIFs were typical nuclear transcription factors with transcriptional activation ability. Expression analysis demonstrated that MdPIF genes had different gene expression patterns for various abiotic factors. Moreover, overexpressed MdPIF4 reduced the sensitivity of apple calluses to abscisic acid (ABA). Our work lays foundations for further investigation of PIF functions in plant growth and development in apples.

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Apple F-Box Protein MdMAX2 Regulates Plant Photomorphogenesis and Stress Response

Cited by 35 publications

References 54 publications

The bZIP transcription factor MdHY5 regulates anthocyanin accumulation and nitrate assimilation in apple

The bZIP transcription factor MdHY5 regulates anthocyanin accumulation and nitrate assimilation in apple

Genome-wide analysis and characterization of F-box gene family in Gossypium hirsutum L

Identification of Phytochrome-Interacting Factor Family Members and Functional Analysis of MdPIF4 in Malus domestica

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