Apple latent spherical virus vector-induced flowering for shortening the juvenile phase in Japanese gentian and lisianthus plants

Fekih, Rym; Yamagishi, Noriko; Yoshikawa, Nobuyuki

doi:10.1007/s00425-016-2498-2

Cited by 14 publications

(10 citation statements)

References 58 publications

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“…In gentian, a virus-induced gene silencing system using Apple latent spherical virus (ALSV) vector has been successfully used for the functional analysis of genes like the class C MADS-box gene, GsAG1 , which is associated with the morphological change from single to double flowers ( Nakatsuka et al 2015 ), and W14/15 , which is associated with winter hardiness required for overwintering ( Hikage et al 2016 ). Furthermore, infection with the ALSV vector harboring FLOWERING LOCUS T ( FT ) has been shown to accelerate flowering in gentian and lisianthus plants ( Fekih et al 2016 ). These results indicate that the ALSV vector is a powerful tool for the study of basic gene function and/or promoting flowering.…”

Section: Use Of Virus Vectors For Gentian Researchmentioning

confidence: 99%

Development of basic technologies for improvement of breeding and cultivation of Japanese gentian

et al. 2018

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Japanese gentians are the most important ornamental flowers in Iwate Prefecture and their breeding and cultivation have been actively conducted for half a century. With its cool climate and large hilly and mountainous area, more than 60% of gentian production in Japan occurs in Iwate Prefecture. Recent advances in gentian breeding and cultivation have facilitated the efficient breeding of new cultivars; disease control and improved cultivation conditions have led to the stable production of Japanese gentians. Molecular biology techniques have been developed and applied in gentian breeding, including the diagnosis of viral diseases and analysis of physiological disorders to improve gentian production. This review summarizes such recent approaches that will assist in the development of new cultivars and support cultivation. More recently, new plant breeding techniques, including several new biotechnological methods such as genome editing and viral vectors, have also been developed in gentian. We, therefore, present examples of their application to gentians and discuss their advantages in future studies of gentians.

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Section: Use Of Virus Vectors For Gentian Researchmentioning

confidence: 99%

Development of basic technologies for improvement of breeding and cultivation of Japanese gentian

et al. 2018

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“…Horticultural plants, such as soybean and Eustoma infected by the ALSV vector expressing AtFT generate flowers earlier than normal ( Figure 4B and C) [33,34]. Such early-flowering plants often set seeds that germinate normally to generate next-generation seedlings.…”

Section: Atft Genementioning

confidence: 99%

ALSV Vector Substantially Shortens Generation Time of Horticultural Plants

Kasajima¹,

Li²,

Yamagishi³

et al. 2017

Plant Engineering

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Flowering of plants is tightly regulated by both plant maturity and seasons in the year. Now that the Flowering Locus T (FT) gene has been revealed to encode the flowering hormone florigen, researchers are seeking to regulate and modify flowering behaviours by using florigen as a genetic tool. In place of transgenic approaches, Apple latent spherical virus (ALSV) vector was successful in promoting flowering of both model plants (Arabidopsis and tobacco), and fruit trees (e.g. apple, pear, and loquat), vegetables (e.g. tomato and cucumber), legumes (e.g. soybean), and ornamental flowers (e.g. petunia, Japanese gentian and Eustoma). In so doing, FT was expressed and/or TFL1 was suppressed by the ALSV vector. ALSV is a latent (non-pathogenic) virus isolated from an apple tree. After induction of flowering and seed production in crops, ALSV is not transferred to most of the next-generation seedlings, or it can be artificially removed from the infected plant by incubation at high temperature. Thus, the generation times of horticultural plants are approximately halved, and the generation time of apple plants is substantially shortened to within one year. Hence, ALSV technology is expected to be useful as a part of New Plant Breeding Techniques (NPBT) for agricultural application.

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“…ALSV is a spherical virus with a diameter of approximately 25 nm originally isolated from an apple tree and is composed of two RNA genome segments (RNA1 and RNA2) and three types of coat proteins (Vp25, Vp20, and Vp24) [39][40][41]. The apple is the only natural host of ALSV; however, ALSV has a relatively wide range of hosts, and it can experimentally infect not only herbaceous plants such as Solanaceae, Cucurbitaceae, Fabaceae, and Gentianaceae but also fruit trees belonging to Rosaceae [26][27][28][29][30][31][32][33][34][35][36][37][38]. ALSV is characterized by latent infection causing no symptoms in almost all host plants and invasion of the shoot apical meristem of infected plants.…”

Section: Apple Latent Spherical Virus (Alsv) Vectorsmentioning

confidence: 99%

“…Recently, we constructed apple latent spherical virus (ALSV) vectors by adding cloning sites to the ALSV genome. The ALSV vector system can be used for the expression of a foreign gene and VIGS in various plant species [26][27][28][29][30][31][32][33][34][35][36][37][38].…”

Section: Introductionmentioning

confidence: 99%

A New Plant Breeding Technique Using ALSV Vectors to Shorten the Breeding Periods of Fruit Trees

Yamagishi¹,

Yoshikawa²

2016

Genetic Engineering - An Insight Into the Strategies and Applications

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Fruit trees have a long juvenile phase. For example, the juvenile phase of apple lasts for 6-12 years and is a serious constraint for creating new varieties by breeding based on crossing and selection. In this chapter, we report a novel technology using the apple latent spherical virus (ALSV) vector to accelerate flowering time and life cycle in apple and pear seedlings. Inoculation of apple and pear cotyledons immediately after germination with ALSV-AtFT/ MdTFL1 concurrently expressing Arabidopsis FLOWERING LOCUS T (AtFT) gene and suppressing apple TERMINAL FLOWER 1-1 (MdTFL1-1) gene can shorten the period from seeding to flowering to 1.5-3 months after germination and generation times in order to obtain next-generation seeds in 1 year or less. Most next-generation seedlings obtained from ALSV vector-infected plants were free of the virus. We also developed a method for eliminating ALSV vectors from infected apple and pear plants by only high-temperature treatment. A method combining the promotion of flowering in apple and pear by ALSV vector with an ALSV elimination technique is expected to see future application as a new plant breeding technique that can significantly shorten the breeding periods of apple and pear.

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Apple latent spherical virus vector-induced flowering for shortening the juvenile phase in Japanese gentian and lisianthus plants

Cited by 14 publications

References 58 publications

Development of basic technologies for improvement of breeding and cultivation of Japanese gentian

Development of basic technologies for improvement of breeding and cultivation of Japanese gentian

ALSV Vector Substantially Shortens Generation Time of Horticultural Plants

A New Plant Breeding Technique Using ALSV Vectors to Shorten the Breeding Periods of Fruit Trees

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