Apple (Malus domestica Borkh.): In Vitro Induction of Androgenesis

Milewska-Pawliczuk, E.

doi:10.1007/978-3-642-61499-6_11

Cited by 4 publications

(3 citation statements)

References 16 publications

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“…These three culture media were already successfully used for C. japonica anther culture (Pedroso-Ubach 1991), However, the presence of BA in MS10 medium, the best for embryo formation from anthers (Pedroso Ubach 1991), only enabled the formation of microspore-derived callus when used for the induction and maturation stages. The death of microspore-derived embryos has also been reported for other woody species (Milewska-Pawhiczuk & Kubicki 1977;Milewska-Pawliczuk 1990;Zhang et al, 1990) and is frequently associated with an inadequate selection of the culture medium. Microspore embryogenesis occurred only when microspores were cultured in the presence of 2,4-D and kinetin (MS6 and MS9 media) and were transferred to MS10 medium for further development.…”

Section: Discussionmentioning

confidence: 73%

Induction of microspore embryogenesis in Camellia japonica cv. Elegans

Pedroso¹,

Pais²

1994

Plant Cell Tiss Organ Cult

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Three methods of microspore culture were tested for the induction of microspore embryogenesis in Camellia japonica L. cv. Elegans. Culture was performed on 17 different media consisting of Murashige and Skoog (MS) and N6 basal media with different combinations of carbon, growth regulators, serine and glutamine. Microspore suspensions plated over solid MS medium containing 4.5 ~tM 2,4-dichlorophenoxyacetic acid and 0.5 ttlVl kinetin, with sucrose (MS6) or glucose (MS9) were seen as the best culture conditions for induction of embryogenesis. The development of microspore derived proembryos was obtained in MS medium supplemented with 2.2 ~tM N 6benzyladenine (MS 10) and reached the highest level when the microspores were cultured in MS6 inducing medium. The development of microspore-derived embryos ceased at the maturation stage.Abbreviations: BA -N6-benzyladenine, 2,4-D -2,4-dichlorophenoxyacetic acid

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Section: Discussionmentioning

confidence: 73%

Induction of microspore embryogenesis in Camellia japonica cv. Elegans

Pedroso¹,

Pais²

1994

Plant Cell Tiss Organ Cult

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“…Malus domestica (L.) Borkh. (Milewska-Pawliczuk 1990, Höfer 1999. El estadio de desarrollo del polen más adecuado para inducir la embriogénesis también varía ampliamente.…”

Section: ) Yunclassified

“…Differences in androgenic response within species have been widely reported in fruit trees including Carica papaya (Tsay and Su 1985), Citrus (Germanà and Reforgiato 1997) and Malus domestica (L.) Borkh. (Milewska-Pawliczuk 1990;Höfer et al 1999). The stage of microspore development also plays a critical role.…”

Section: Introductionmentioning

confidence: 99%

Desarrollo de métodos biotecnológicos aplicados a la mejora genética del níspero japonés (Eriobotrya japonica (Thunb.) Lindl.)

Villarroya¹

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En primer lugar quiero agradecerle a Gerardo Llácer la oportunidad que me dio para poder realizar mi tesis doctoral en el IVIA. A mis directoras, Marisa Badenes y Mar Naval, por depositar su confianza en mí, por todo su esfuerzo, tiempo y dedicación en mi formación y la planificación, desarrollo y finalización de esta tesis. A todo el grupo de frutales, del primero al último, a los que están y a los que no, por todo vuestro apoyo y colaboración; 'I get by with a little help from my friends'.

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