Apple procyanidins induce hyperpolarization of rat aorta endothelial cells via activation of K+ channels

Byun, Eui Baek; Korematsu, Sachiko; Ishikawa, Takeko; Nishizuka, Taichi; Ohshima, Shunji; Kanda, Tomomasa; Matsui, Toshiro

doi:10.1016/j.jnutbio.2010.12.005

Cited by 12 publications

(5 citation statements)

References 45 publications

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“…The compounds of the procyanidin (oligomeric flavan-3-ol) class have been identified in the plants of the family Rosaceae [42] and in various apple cultivars [24,40]. Procyanidins isolated from apples have an antineoplastic effect [43], a strong antioxidant effect [44], an anti-inflammatory effect [45], a cholesterol-and triglyceride-lowering effect [37], and a positive effect on the cardiovascular system [46,47], and therefore they are among the most promising phenolic compounds in apples. In the present study, procyanidins B1 and B2 were identified and quantified in the ethanol extracts of apple fruit samples.…”

Section: Compoundmentioning

confidence: 99%

Application of an Optimized HPLC Method for the Detection of Various Phenolic Compounds in Apples from Lithuanian Cultivars

Liaudanskas

Viškelis

Jakštas

et al. 2014

Journal of Chemistry

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A specific analytical procedure including sample preparation and HPLC analysis was developed and validated for the detection of phenolic compounds in the samples of different apples from popular Lithuanian cultivars “Aldas,” “Auksis,” “Ligol,” and “Šampion.” The conditions for phenol extraction were optimized: the solvent of the extraction was 70% (v/v) ethanol, and the extraction was performed in an ultrasound bath for 20 min at the temperature of 40°C. The HPLC mobile phase consisted of 2% (v/v) acetic acid in water and 100% (v/v) acetonitrile. Using the HPLC technique, 11 analytes were identified, and their specificity was confirmed: procyanidin B1, (+)-catechin, chlorogenic acid, procyanidin B2, (−)-epicatechin, rutin, hyperoside, isoquercitrin, avicularin, quercitrin, and phloridzin. Chlorogenic acid was the major component in “Aldas,” “Auksis,” and “Ligol” and procyanidin B2 in “Šampion.” Hyperoside and avicularin were the dominant compounds of all the identified quercetin derivatives in “Aldas” and “Auksis;” hyperoside in “Šampion;” and quercitrin in “Ligol.” The total content of phenolic compounds varied from 1641.0 ± 47.9 μg/g (cv. “Ligol”) to 4291.3 ± 154.2 μg/g (cv. “Aldas”).

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Section: Compoundmentioning

confidence: 99%

Application of an Optimized HPLC Method for the Detection of Various Phenolic Compounds in Apples from Lithuanian Cultivars

Liaudanskas

Viškelis

Jakštas

et al. 2014

Journal of Chemistry

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“…We thus propose that proanthocyanidins should not affect the properties of pre-and post-synaptic ion channels in the spinal cord. However, it's shown that proanthocyanidins induce hyperpolarization of rat aorta endothelial cells via multiple activations of K + channels and play a protective role in Ca 2+ influx into endothelial cells (Byun et al, 2012). Proanthocyanidins are also reported to help maintain the intracellular Ca 2+ -homeostasis after NMDA receptor overactivation, protecting neurons from Ca 2+ -induced adverse effects (Franchi et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Proanthocyanidins Inhibit the Transmission of Spinal Pain Information Through a Presynaptic Mechanism in a Mouse Inflammatory Pain Model

Fan

Zhu

et al. 2022

Front. Neurosci.

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Inflammatory pain is one of the most common symptoms of clinical pain that seriously affects patient quality of life, but it currently has limited therapeutic options. Proanthocyanidins, a group of polyphenols enriched in plants and foods, have been reported to exert anti-inflammatory pain-alleviating effects. However, the mechanism by which proanthocyanidins relieve inflammatory pain in the central nervous system is unclear. In the present study, we observed that intrathecal injection of proanthocyanidins inhibited mechanical and thermal pain sensitivity in mice with inflammatory pain induced by Complete Freund’s Adjuvant (CFA) injection. Electrophysiological results further showed that proanthocyanidins inhibited the frequency of spontaneous excitatory postsynaptic currents without affecting the spontaneous inhibitory postsynaptic currents or the intrinsic properties of parabrachial nucleus-projecting neurons in the spinal cord. The effect of proanthocyanidins may be mediated by their inhibition of phosphorylated activation of the PI3K/Akt/mTOR pathway molecules in dorsal root ganglia neurons. In summary, intrathecal injection of procyanidin induces an obvious anti-inflammatory pain effect in mice by inhibiting peripheral excitatory inputs to spinal neurons that send nociceptive information to supraspinal areas.

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“…58 Similar studies have demonstrated that vasorelaxation caused by apple procyanidins can be blocked by 4-AP as well as by other K + channel blockers. 23,24 It appears that these fruit-derived polyphenols may act as relaxing factors by activating Kv (and possibly other K + ) channels and causing hyperpolarisation. The full extent of the signalling mediators involved in this mechanism requires further study however.…”

Section: Discussionmentioning

confidence: 99%

“…[18][19][20][21][22] However, some studies have implicated a role for K + channels in mediating the vasorelaxant effects of polyphenols. [23][24][25] Given the crucial role that potassium channels play in vascular smooth muscle, 26 we hypothesise that any effect of blueberry juice on the vascular endothelium may be mediated, at least in part, by activation of K + channels. Indeed, some studies have linked poor dietary habits to dysregulation of potassium channels and associated hypertension.…”

Section: Introductionmentioning

confidence: 99%

Blueberry juice causes potent relaxation of rat aortic rings via the activation of potassium channels and the H₂S pathway

et al. 2013

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The objective of this study was to investigate the in vitro effects of blueberry juice on healthy rat aortic rings, and to explore the roles of potassium channels and of the hydrogen sulphide (H(2)S) pathway in mediating the effects of blueberry juice. Firstly, the antioxidant capacity of blueberry juice was compared to other popular juice drinks using the Folin-Ciocalteu and the DPPH assays. Blueberry juice had significantly higher total polyphenol content than any of the other drinks studied (p < 0.01). The effect of blueberry juice on noradrenaline-contracted aortic rings was then observed, and the juice caused significant inhibition of noradrenaline-induced contractions (p < 0.01). Voltage-gated potassium channel (Kv) blockers 4-aminopyridine (1 mM) and 3,4-diaminopyridine (1 mM), as well as the cystathionine γ-lysase (CSE) inhibitor d,l-propargylglycine (2 mM) were then utilised to elucidate the role of Kv channels and the CSE/H(2)S pathway. Kv channel blocker 3,4-diaminopyridine caused significant blockade at 1/100 and 1/50 dilutions of juice (p < 0.01), whilst 4-aminopyridine caused significant blockade of the 1/100 dilution of blueberry juice (p < 0.05). In addition, d,l-propargylglycine potently inhibited the effect of 1/100 and 1/50 dilutions of blueberry juice (p < 0.01). This study indicates that blueberry juice has potent vasorelaxing properties, and thus may be a useful dietary agent for the prevention and treatment of hypertension. This study also provides strong evidence that Kv channels and the CSE/H(2)S pathway may be responsible, at least in part, for mediating the effects of blueberry juice.

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Apple procyanidins induce hyperpolarization of rat aorta endothelial cells via activation of K+ channels

Cited by 12 publications

References 45 publications

Application of an Optimized HPLC Method for the Detection of Various Phenolic Compounds in Apples from Lithuanian Cultivars

Application of an Optimized HPLC Method for the Detection of Various Phenolic Compounds in Apples from Lithuanian Cultivars

Proanthocyanidins Inhibit the Transmission of Spinal Pain Information Through a Presynaptic Mechanism in a Mouse Inflammatory Pain Model

Blueberry juice causes potent relaxation of rat aortic rings via the activation of potassium channels and the H₂S pathway

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Apple procyanidins induce hyperpolarization of rat aorta endothelial cells via activation of K+ channels

Cited by 12 publications

References 45 publications

Application of an Optimized HPLC Method for the Detection of Various Phenolic Compounds in Apples from Lithuanian Cultivars

Application of an Optimized HPLC Method for the Detection of Various Phenolic Compounds in Apples from Lithuanian Cultivars

Proanthocyanidins Inhibit the Transmission of Spinal Pain Information Through a Presynaptic Mechanism in a Mouse Inflammatory Pain Model

Blueberry juice causes potent relaxation of rat aortic rings via the activation of potassium channels and the H2S pathway

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Blueberry juice causes potent relaxation of rat aortic rings via the activation of potassium channels and the H₂S pathway