Applicability of a yeast oestrogen screen for the detection of oestrogen-like activities in environmental samples

Rehmann, Klaus; Schramm, Karl‐Werner; Kettrup, A.

doi:10.1016/s0045-6535(98)00562-1

Cited by 90 publications

(55 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…But in most cases, at least more than 1 day is needed for detection (Routledge and Sumpter, 1996;Arnold et al, 1996;Coldham et al, 1997;Miller et al, 2001;Boever et al, 2001). As an improvement of the screening procedure, we were able to accomplish the detection of a batch of samples within 4-6 h. Results in Table 2 show that the EC50-based ratios of the estrogenic activities of the compounds in the study were comparable to values found with another yeast construct (Gaido et al, 1997;Rehmann et al, 1999;Murk et al, 2002).…”

Section: Resultsmentioning

confidence: 52%

Differing estrogen activities in the organic phase of air particulate matter collected during sunny and foggy weather in a Chinese city detected by a recombinant yeast bioassay

Wang

Xie

et al. 2004

Atmospheric Environment

Self Cite

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 52%

Differing estrogen activities in the organic phase of air particulate matter collected during sunny and foggy weather in a Chinese city detected by a recombinant yeast bioassay

Wang

Xie

et al. 2004

Atmospheric Environment

Self Cite

View full text Add to dashboard Cite

“…For compounds yielding responses less than 20% of the positive control value, but showing a significant difference from the negative control, the REC20 or RIC20 values were greater than the minimum value (>minimum value) having significant difference (Table 1). The dose-response curve was generated using a four-parameter logistic model based on the Marquardt-Levenberg algorithm (Sigmaplot 4.0, SPSS, Chicago, IL, USA) (Rehmann et al, 1999).The four-parameter logistic equation gives a dose response curve fitted to the measured values REC20 as well as the opportunity to calculate RIC20 values for antagonistic assays.…”

Section: Discussionmentioning

confidence: 99%

In vitro profiling of endocrine disrupting effects of phenols

Wang

2010

Toxicology in Vitro

116

View full text Add to dashboard Cite

a b s t r a c tSome phenols have been suspected to modulate the endocrine systems of wildlife and humans, but less is known about their interactions with different types of nuclear receptors. In this study, the ability of 2-tert-butylphenol, 2-isopropylphenol, 4-tert-octylphenol (4-t-OP), 2,4-dichlorophenol (2,4-DCP), 3,4-dichlorophenol (3,4-DCP), pentachlorophenol (PCP), bisphenols A (BPA), tetrabromobisphenol A (TBBPA), tetrachlorobisphenol A (TCBPA) and 4-phenylphenol to activate estrogen receptor (ER), androgen receptor (AR), progesterone receptor (PR) and estrogen-related receptor (ERR) were determined using a set of recombined yeast strains. It was found that 4-t-OP, 3,4-DCP, PCP, BPA, TBBPA, TCBPA and 4-phenylphenol were ERa agonists, while 4-t-OP, PCP and 4-phenylphenol showed ERa antagonistic activities. 2-tertButylphenol, 4-t-OP, 2-isopropylphenol, 2,4-DCP, 3,4-DCP, BPA, TCBPA and 4-phenylphenol were antagonists for AR, whereas none of the compounds studied were found to be an AR agonist. TCBPA, TBBPA and PCP were PR antagonists, and 2-tert-butylphenol, 3,4-DCP, 4-t-OP, 4-phenylphenol and 2-isopropylphenol were weak inhibitors on expression under control of the PR. None of the phenols were PR agonists. 2-tert-Butylphenol, 4-t-OP and PCP were ERRc inverse agonists, while 2,4-DCP, 3,4-DCP, PCP, BPA, TBBPA and TCBPA exhibited the ability to reverse the ERR inhibition induced by 4-hydroxytamoxifen. Based on the functional agonistic or antagonistic receptor-mediated effects, we further discussed the possible action mechanisms of these phenols as endocrine disrupting chemicals.

show abstract

“…The dose-response curve was generated using a four-parameter logistic model based on the Marquardt-Levenberg algorithm (Sigmaplot 4.0®, SPSS, Chicago, IL, USA) (Rehmann et al 1999).…”

Section: Discussionmentioning

confidence: 99%

T-screen and yeast assay for the detection of the thyroid-disrupting activities of cadmium, mercury, and zinc

Liu

Ren

et al. 2016

Environ Sci Pollut Res

View full text Add to dashboard Cite

In the present study, a two-hybrid yeast bioassay and a T-screen were used to screen for the thyroid receptor (TR)-disrupting activity of select metallic compounds (CdCl 2 , ZnCl 2 , HgCl 2 , CuSO 4 , MnSO 4 , and MgSO 4 ). The results reveal that none of the tested metallic compounds showed TRagonistic activity, whereas ZnCl 2 , HgCl 2 , and CdCl 2 demonstrated TR antagonism. For the yeast assay, the dose-response relationship of these metallic compounds was established, and the concentrations producing 20 % of the maximum effect of ZnCl 2 , HgCl 2 , and CdCl 2 were 9.1 × 10 −5 , 3.2 × 10 −6 , and 1.2 × 10 −6 mol/L, respectively. The T-screen also supported the finding that ZnCl 2 , HgCl 2 , and CdCl 2 decreased the cell proliferation at concentrations ranging from 10 −6 to 10 −4 mol/ L. Furthermore, the thyroid-disrupting activity of metallic compounds in environmental water samples collected from the Guanting Reservoir, Beijing, China was evaluated. Solid-phase extraction was used to separate the organic extracts, and a modified two-hybrid yeast bioassay revealed that the metallic compounds in the water samples could affect thyroid hormone-induced signaling by decreasing the binding of the thyroid hormone. The addition of ethylenediaminetetraacetic acid (30 mg/L) could eliminate the effects. Thus, the cause(s) of the thyroid toxicity in the water samples appeared to be partly related to the metallic compounds.

show abstract

Applicability of a yeast oestrogen screen for the detection of oestrogen-like activities in environmental samples

Cited by 90 publications

References 18 publications

Differing estrogen activities in the organic phase of air particulate matter collected during sunny and foggy weather in a Chinese city detected by a recombinant yeast bioassay

Differing estrogen activities in the organic phase of air particulate matter collected during sunny and foggy weather in a Chinese city detected by a recombinant yeast bioassay

In vitro profiling of endocrine disrupting effects of phenols

T-screen and yeast assay for the detection of the thyroid-disrupting activities of cadmium, mercury, and zinc

Contact Info

Product

Resources

About