Applicability of ISAP, ISSR and SSR markers in tomato breeding programs

Abstract

Domesticated crops are characterized by narrow genetic base reflecting one or more bottlenecks during millennia-long selection. As a result, current breeding programs are limited in available germplasm and are forced to deal with incremental improvements of yield, resistance, nutritional value, etc. Since the establishment of modern genetics and biotechnology, several new approaches have emerged to extend the genetic base and germplasm improvement. Among these methods, induced mutagenesis appeared as most useful conventional breeding tool. Although, its successful application currently requires good knowledge of modern molecular tools. In this paper we will make an attempt to overview SSR, ISSR and ISAP techniques as well as to offer examples of their application in tomato breeding programs.

Application of inter-SINE amplified polymorphism (ISAP) markers for genotyping of Cucumis melo accessions and its transferability in Coleus spp

“…Wenke et al (2015) successfully developed inter-SINE amplified polymorphism (ISAP) marker for plant genotyping. The ISAP markers developed by various groups produced genotype-specific fingerprints at high resolutions, thus allowing cost-efficient, robust, and rapid plant genotyping (Seibt et al 2012;Wenke et al 2015;Diekmann et al 2017;Pantchev et al 2019).…”