Applicability of solid-phase microextraction followed by on-fiber silylation for the determination of estrogens in water samples by gas chromatography–tandem mass spectrometry

Carpinteiro, J.; Quintana, José Benito; Rodrı́guez, I.; Carro, A.M.; Lorenzo, R.A.; Cela, Rafael

doi:10.1016/s0021-9673(04)01082-9

Cited by 75 publications

(8 citation statements)

References 19 publications

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“…Estrogens are typically isolated from liquid samples by offline SPE in combination (online) with a selected identification technique . Liquid–liquid extraction , solid‐phase microextraction , and stir bar sorptive extraction are less popular methods of steroid isolation. Chromatographic methods are most frequently used to separate and identify steroid hormones in liquid samples .…”

Section: Introductionmentioning

confidence: 99%

Isolation and determination of estrogens in water samples by solid-phase extraction using molecularly imprinted polymers and HPLC

Sadowski

Gadzała‐Kopciuch

2013

J. Sep. Science

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Advanced SPE with molecularly imprinted polymers (MIP) was used in this study. A noncovalent imprinting approach was applied to separate 17β-estradiol, estriol, and estrone from water samples. Polymer material was prepared by bulk polymerization with methacrylic acid as a functional monomer, divinylbenzene and ethyleneglycol dimethacrylate as crosslinkers, and acetonitrile, acetonitrile/toluene (3:1, v/v) or isooctane/toluene (1:99, v/v) as a porogen. We also prepared an MIP film on a silica gel surface with methacrylic acid and ethyleneglycol dimethacrylate as monomers and acetonitrile as a solvent. Qualitative and quantitative hormone analyses were carried out by HPLC with various detection techniques, including UV/visible spectroscopic detection (diode array detection) and electrochemical detection (CoulArray). The results of the study indicate that MIP technology is an excellent method for the quality control of estrogens in environmental analyses with a low quantification limit for 17β-estradiol of around 26 (diode array detection) and 0.25 ng/mL (electrochemical detection). The proposed method was found to be suitable for routine determinations of the analyzed compound in environmental laboratories.

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Section: Introductionmentioning

confidence: 99%

Isolation and determination of estrogens in water samples by solid-phase extraction using molecularly imprinted polymers and HPLC

Sadowski

Gadzała‐Kopciuch

2013

J. Sep. Science

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“…For isolation of E2 and purification of milk samples, several preparation procedures have been developed mainly including solid-phase extraction (SPE) [9], solid phase microextraction (SPME) [10], liquid-liquid extraction (LLE) [11], and matrix solid-phase dispersion (MSPD) [12]. Each of these methods has its advantages, but some drawbacks exist in these strategies.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of mimic molecularly imprinted ordered mesoporous silica adsorbent by thermally reversible semicovalent approach for pipette-tip solid-phase extraction-liquid chromatography fluorescence determination of estradiol in milk

Wang

Yan

Yang

et al. 2016

Journal of Chromatography A

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“…The use of harmful compounds and large amount of solvents can be additional environment pollutants. As a means of overcoming these problems, miniaturised extraction techniques have been developed such as solid-phase micro-extraction (SPME) [1,2] and liquid phase micro-extraction (single-drop LPME) [3]. In case of LPME technologies, a number of subcategorised techniques such as single drop micro-extraction (SDME), continuous flow micro-extraction (CFME), directly suspended droplet micro-extraction (DSDME), and hollow fibre-liquid phase micro-extraction (HF-LPME) have been developed for better methodological validation [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

The application of hollow fibre-liquid phase micro-extraction on the bioassay experiment of oestrogen chemicals

Kim

Lee

et al. 2012

International Journal of Environmental Analytical Chemistry

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The object of this study was to improve pre-concentration techniques using hollow fibre protected-liquid phase micro-extraction (LPME) for its application on biotic samples and limited environmental samples provided in small volumes. Five parameters, including extraction time, type of solvent, extents of agitation, fibre length and salt content of HF-LPME were optimised for the yeast twohybrid assay and gas chromatography-mass spectrometer (GC/MS) chemical analysis. The results of chemical analysis were compared to the results of a bioassay using the yeast two-hybrid system to analyse endocrine-disrupting chemicals (EDCs). For the yeast two-hybrid assay, the optimised parameters comprised of an extraction time of 40 min, toluene as the extraction solvent, a stirring rate of 500 rpm, a fibre length of 2.0 cm and a salt concentration of 20% (w/v). For GC/MS analysis, HF-LPME conditions were optimised with an extraction time of 40 min, toluene: dichloromethane at a ratio of 1 : 1 (v : v) as the extraction solvent, a stirring rate of 700 rpm, a fibre length of 2.0 cm and a salt concentration of 30% (w/v). The results indicated that fibre length, extraction time, and degree of agitation were major contributing parameters for HF-LPME in EDCs analysis. Consequently, bioassays such as yeast two-hybrid assay could be easily, rapidly, and inexpensively conducted with small volume of sample using HF-LPME for the measurement of oestrogenic activity.

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Applicability of solid-phase microextraction followed by on-fiber silylation for the determination of estrogens in water samples by gas chromatography–tandem mass spectrometry

Cited by 75 publications

References 19 publications

Isolation and determination of estrogens in water samples by solid-phase extraction using molecularly imprinted polymers and HPLC

Isolation and determination of estrogens in water samples by solid-phase extraction using molecularly imprinted polymers and HPLC

Synthesis of mimic molecularly imprinted ordered mesoporous silica adsorbent by thermally reversible semicovalent approach for pipette-tip solid-phase extraction-liquid chromatography fluorescence determination of estradiol in milk

The application of hollow fibre-liquid phase micro-extraction on the bioassay experiment of oestrogen chemicals

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