Application and bioactive properties of <i><scp>CaTI</scp></i>, a trypsin inhibitor from <i>Capsicum annuum</i> seeds: membrane permeabilization, oxidative stress and intracellular target in phytopathogenic fungi cells

Silva, Marciele S.; Ribeiro, Suzanna F. F.; Taveira, Gabriel Bonan; Rodrigues, Rosana; Fernandes, Kátia Valevski Sales; Carvalho, André O.; Vasconcelos, Ilka M.; Mello, Érica O.; Gomes, Valdirene Moreira

doi:10.1002/jsfa.8243

Cited by 26 publications

(19 citation statements)

References 54 publications

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“…Santos et al [13] isolated the Fa5 fraction of C. annuum fruits, which showed high antimicrobial activity only against species of the genus Fusarium. In contrast, Silva et al [28] showed that CaTI, a proteinase inhibitor isolated from C. annuum seeds, was able to inhibit only the growth of fungi of the genus Colletotrichum. Slazak et al [32] isolated several cytotoxins from Viola odorata, namely, cyO2, cyO3, cyO13 and cyO19, and showed that all these showed inhibitory activity against all fungi tested (F. oxysporum, F. graminearum, F. culmorum, Mycosphaerella fragariae, and Botrytis cinerea).…”

Section: Discussionmentioning

confidence: 92%

“…This fraction was separated by C18 reversed-phase column ( Figure 1B) and the ten fractions obtained (Figure 2), presented antifungal activity against four different species of phytopathogenic fungi (Figure 3). In recent years, our group has reported several plant AMPs as potent growth inhibitors of microorganisms [27][28][29]. Games et al [27] purified a defensin from Phaseolus vulgaris seeds, called PvD1, which showed inhibitory activity against yeasts and phytopathogenic fungi at concentration of 100 μg.ml −1 .…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi

et al. 2020

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In recent years, the antimicrobial activity of peptides isolated from a wide variety of organs from plant species has been reported. However, few studies have investigated the potential of antimicrobial peptides (AMPs) found in fruits, especially Capsicum chinense (pepper). This study aimed to purify and characterize peptides from Capsicum chinense fruits and evaluate their inhibitory activities against different phytopathogenic fungi and also analyze the possible mechanisms of action involved in microbial inhibition. After fruit protein extraction and high-performance liquid chromatography (HPLC), different fractions were obtained, named F1 to F10. Peptides in the F4 and F5 fractions were sequenced and revealed similarity with the plant antimicrobial peptides like non-specific lipid transfer proteins and defensin-like peptide. The F4 and F5 fractions presented strong antimicrobial activity against the fungus Fusarium solani and F. oxysporum, causing toxic effects on these fungi, leading to membrane permeabilization, endogenous reactive oxygen species increase, activation of metacaspase and loss of mitochondrial function.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi

et al. 2020

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“…This protein had the ability to permeabilize the plasma membrane of Saccharomyces cerevisia e and Candida albicans cells after 24 h of growth. Silva et al [7] reported growth inhibition of the fungi C. gloeosporioides and C. lindemuthianum by the CaTI inhibitor, in addition to showing the ability of the protein to permeabilize the membrane of all the tested fungi, what indicates the importance of protease inhibitors in the defense of plants against several pathogens. The protease inhibitors are constitutively present in all plant kingdoms, being mainly located in the reserve tissues, in addition to being produced in response to injuries caused by insects or even by fungi and bacteria [41–43].…”

Section: Resultsmentioning

confidence: 99%

“…These AMPs can be expressed in high concentrations upon the aggression of some pathogen or upon exposure to biotic or abiotic stresses. Effectively, antimicrobial peptides tend to act directly in the region of injury against the aggressor and can be found in several plant organs, such as seeds, reproductive organs, tubers, fruits and flowers, and in almost all plant species [7].…”

Section: Introductionmentioning

confidence: 99%

Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides

et al. 2019

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There are several phytosanitary problems that have been causing serious damage to the Capsicum crops, including anthracnose. Upon attack by certain pathogens, various protein molecules are produced, which are known as proteins related to pathogenesis (PR proteins), including antimicrobial peptides such as protease inhibitors, defensins and lipid transfer proteins (LTPs). The objective of this work is to identify antimicrobial proteins and/or peptides of two genotypes from Capsicum annuum fruits infected with Colletotrichum gloeosporioides. The fungus was inoculated into Capsicum fruits by the deposition of a spore suspension (106 conidia ml−1), and after 24 and 48 h intervals, the fruits were removed from the humid chamber and subjected to a protein extraction process. Protein analysis of the extracts was performed by tricine gel electrophoresis and Western blotting. The distinctive bands between genotypes in the electrophoresis profiles were subjected to mass spectrometry sequencing. Trypsin inhibition assays, reverse zymographic detection of protease inhibition and β-1,3-glucanase activity assays were also performed and extracts were also tested for their ability to inhibit the growth of C. gloeosporioides fungi ‘in vitro’. There were several low molecular weight proteins in all treated samples, and some treatments in which antimicrobial peptides such as defensin, lipid transfer protein (LTP) and protease inhibitor have been identified. It was shown that the green fruits are more responsive to infection, showing the production of antimicrobial peptides in response to injury and inoculation of the fungus, what did not occur in ripe fruits under any treatment.

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“…against various filamentous fungi and found that all eight fractions were able to inhibit the growth of Fusarium lateritium, Fusarium solani and Fusarium oxysporum but were not able to inhibit the growth of C. gloeosporioides . Silva et al [21] tested CaTI , a protease inhibitor obtained from C. annuum seeds, on the growth of fungi Colletotrichum lindemuthianum, C. gloeosporioides, F. solani and F. oxysporum and found inhibition against fungi of the genus Colletotrichum , but not for those of the genus Fusarium . In microscopic analysis, it was concluded that CaTI reduced the growth of the mycelium of C. gloeosporioides .…”

Section: Discussionmentioning

confidence: 99%

Characterization and antifungal activity of a plant peptide expressed in the interaction between Capsicum annuum fruits and the anthracnose fungus

et al. 2019

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Plant defensins are low molecular weight basic peptides ranging from 5 to 7 kDa, with capacity of inhibiting various pathogens, including fungi. They are present in different tissues of plants, including floral parts and fruits of Capsicum sp. The IIF48 extract, present in immature fruits of Capsicum annuum inoculated with C. gloeosporioides, was able to inhibit up to 100% growth ‘in vitro’ of the fungus Colletotrichum gloeosporioides. The main objective of this work was the purification and antifungal activity characterization of a defense-related plant defensin-like isolated of the IIF48 immature fruits extract. The IIF48 extract was subjected to HPLC purification and 13 fractions were obtained, followed by a tricine gel electrophoresis to obtain the protein profile. The different fractions were submitted to a growth inhibition assay against C. gloeosporioides fungus. Fraction 7 (F7) was the most active causing 73% inhibition. Because of the higher F7 activity and the presence of only a peptide of approximately 5 kDa this fraction was subjected to N-terminal sequencing. F7 fraction was carried out plasma membrane permeabilization assays, induction of intracellular ROS production analysis and investigated mitochondrial membrane potential. The F7 fraction showed significant inhibitory activity on the tested fungus, besides promoting membrane permeabilization, induction of endogenous ROS production in Colletotrichum cells and impairing mitochondrial functionality. The first 18 amino acid sequence of the F7 fraction peptide suggests homology to plant-like defensin and was named IIFF7Ca. We also concluded that IIFF7Ca peptide has an effective antimicrobial action against the fungus C. gloeosporioides.

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Application and bioactive properties of CaTI, a trypsin inhibitor from Capsicum annuum seeds: membrane permeabilization, oxidative stress and intracellular target in phytopathogenic fungi cells

Cited by 26 publications

References 54 publications

Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi

Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi

Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides

Characterization and antifungal activity of a plant peptide expressed in the interaction between Capsicum annuum fruits and the anthracnose fungus

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