Application and characterization of magnetic chitosan microspheres for enhanced immobilization of cellulase

Miao, Xixi; Pi, Leilei; Fang, Lei; Wu, Ruizhi; Xiong, Chunhua

doi:10.1080/10242422.2016.1247830

Cited by 21 publications

(7 citation statements)

References 31 publications

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“…According to the authors, both supports (microcomposites and porous gel beads) led to similar glucose production yields (using CMC as substrate) and kept up to 69.9% of the original production yield after reusing the immobilized cellulases for 10 cycles. Chitosan can also be used to modify magnetic nanoparticles, such as Fe 3 O 4 , thus gaining their magnetic properties leading to an easy handling of the enzyme [71,75,76].…”

Section: Chitosanmentioning

confidence: 99%

Strategies towards Reduction of Cellulases Consumption: Debottlenecking the Economics of Lignocellulosics Valorization Processes

et al. 2021

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Lignocellulosic residues have been receiving growing interest as a promising source of polysaccharides, which can be converted into a variety of compounds, ranging from biofuels to bioplastics. Most of these can replace equivalent products traditionally originated from petroleum, hence representing an important environmental advantage. Lignocellulosic materials are theoretically unlimited, cheaper and may not compete with food crops. However, the conversion of these materials to simpler sugars usually requires cellulolytic enzymes. Being still associated with a high cost of production, cellulases are commonly considered as one of the main obstacles in the economic valorization of lignocellulosics. This work provides a brief overview of some of the most studied strategies that can allow an important reduction of cellulases consumption, hence improving the economy of lignocellulosics conversion. Cellulases recycling is initially discussed regarding the main processes to recover active enzymes and the most important factors that may affect enzyme recyclability. Similarly, the potential of enzyme immobilization is analyzed with a special focus on the contributions that some elements of the process can offer for prolonged times of operation and improved enzyme stability and robustness. Finally, the emergent concept of consolidated bioprocessing (CBP) is also described in the particular context of a potential reduction of cellulases consumption.

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Section: Chitosanmentioning

confidence: 99%

Strategies towards Reduction of Cellulases Consumption: Debottlenecking the Economics of Lignocellulosics Valorization Processes

et al. 2021

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“…In recent decades, enzymatic technology has been widely used in textile processing. 1,2 The enzymatic process has the merits of substrate specificity, low energy consumption, eco-friendliness, and mild operating conditions. 3,4 Notwithstanding all these advantages, enzymes often lack operational and storage stability that have limited their widespread application.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Cross-Linked Cellulase Aggregate and Application to Cool Finishing of Cotton Fabric

Bai¹,

Feng²,

Yan-chun³

et al. 2021

AATCC Journal of Research

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Cellulase was immobilized by cross-linked enzyme aggregation to improve the stability of cellulase. The prepared cross-linked cellulase aggregates (CLCAs) and ice silicone oil were used for the cool finishing of cotton fabric. The results showed that the CLCAs extended the cellulase stability compared to free cellulase. The surface softness, smoothness, moisture permeability, and air permeability of the cotton fabric increased after CLCAs and ice silicone oil treatment. Shearing rigidity of the treated sample was 0.44 cN/(cm·deg), bending rigidity was 0.0069 cN cm, and the drape coefficient was 29.3%. Coefficient of kinetic friction of the treated sample was 0.186. The capillary effect of the treated fabric was 12.1 cm/(30 min). Air permeability was 354.3 L/(m2·s). Moisture penetrability was 3.912 g/(m2·d). The thermal and water-vapor resistance were 0.0194 m2·°C/W and 4.691 Pa·m2/W, respectively.

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“…A convenient method for overcoming these issues is immobilization on adequate solid supports, providing increased operational and storage stability and reusability (Vaz et al, 2016). During the last decades, various microbial cellulases were immobilized onto different nanoparticles (Xu et al, 2011;Gokhale et al, 2013;Lima et al, 2017;Han et al, 2018;Simon et al, 2018), magnetic chitosan microspheres (Miao et al, 2016), kaolin (Lima et al, 2019), etc. However, most of these immobilized preparations are not applicable for the saccharification of natural lignocellulosic substrates due to their high prices.…”

Section: Introductionmentioning

confidence: 99%

Hydrolysis of sunflower seed meal lignocellulosic fraction by free and immobilized cellulases

Ćorović

2020

GHTERS

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Lignocellulosic biomass is widely abundant in nature and recognized aspotential renewable energy source. Its efficient transformation into bio-basedfuels is enabled only after adequate pretreatment, followed by enzymaticsacharification and microbial fermentation. Hereby we present application of twocellulase preparations – from Aspergillus niger and Trichoderma reesei (Celluclast®)in treating sunflower seed meal lignocellulosic fraction (SSMLF). Temperature andpH optimums of two enzymes were determined – 52 °C and pH4.8 for A. nigercellulase and 55 °C and pH4.5 for Celluclast®. At optimized conditions, milledSSMLF was hydrolyzed by both biocatalysts. With A. niger cellulase higher initialreaction rates were accomplished and yield of 70 mM glucose equivalent wasobtained with 6 % (w/v) of enzyme after 6 hours. On the other hand, applicationof Celluclast® led to lower initial reaction rates and yielded 25 mM of glucoseequivalent with 10 % (v/v) of enzyme. To ensure cost-effective application ofA. niger cellulase, the possibility of its immobilization on different supports wasinvestigated. By using porous methacrylate-based carrier with C6 spacer arm andprimary amino groups – LifetechTM ECR8409, preparation with highest activity wasproduced. This preparation was successfully applied in saccharification of SSMLFand showed unchanged catalytic efficiency comparing to free enzyme.

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Application and characterization of magnetic chitosan microspheres for enhanced immobilization of cellulase

Cited by 21 publications

References 31 publications

Strategies towards Reduction of Cellulases Consumption: Debottlenecking the Economics of Lignocellulosics Valorization Processes

Strategies towards Reduction of Cellulases Consumption: Debottlenecking the Economics of Lignocellulosics Valorization Processes

Preparation of Cross-Linked Cellulase Aggregate and Application to Cool Finishing of Cotton Fabric

Hydrolysis of sunflower seed meal lignocellulosic fraction by free and immobilized cellulases

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