Application and evaluation of a new cold-stable kinetic Jaffé reagent to the Hitachi 747 for the determination of serum creatinine

Artiss, Joseph D.; Karcher, Raymond E.; Collins, Sandra L.; Zak, Bennie

doi:10.1016/s0026-265x(00)00125-9

Cited by 7 publications

(3 citation statements)

References 11 publications

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“…15 The traditional assay for creatinine, known as the Jaffé reaction, 16 is based on the reaction with picric acid under alkaline conditions to form a highly colored product (Janovsky complex). Enzymatic reactions 17,18 or a modified version of the Jaffé method 19,20 can be used to more accurately measure creatinine in biological fluids when noncreatinine chromogens are present. Uric acid is the major metabolic product of guanine and adenine and serves as a marker for tubular readsorption.…”

Section: Introductionmentioning

confidence: 99%

“…First, all are subject to interferences from commonly occurring biological compounds such as acetoacetate, 27 carbohydrates, 19 or proteins. 20,28 Second, a different assay must be used for each analyte, increasing the time and cost of analysis. In order to overcome these drawbacks, separation methods can be used to analyze multiple renal markers simultaneously.…”

Section: Introductionmentioning

confidence: 99%

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Direct detection of renal function markers using microchip CE with pulsed electrochemical detection

Garcı́a

Henry

2004

Analyst

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Creatinine, creatine, and uric acid are three important compounds that are measured in a variety of clinical assays, most notably for renal function. Traditional clinical assays for these compounds have focused on the use of enzymes or chemical reactions. Electrophoretic microchips have the potential to integrate separation power of capillary electrophoresis with devices that are small, portable, and have the speed of conventional sensors. The development of a microchip CE system for the direct detection of creatinine, creatine, and uric acid is presented. The device uses pulsed amperometric detection (PAD) to detect the nitrogen-containing compounds as well as the easily oxidizable uric acid. Baseline separation of creatinine, creatine and uric acid was achieved using 30 mM borate buffer (pH = 9.4) in less than 200 s. Linear calibration curves were obtained with limits of detection of 80 microM, 250 microM and 270 microM for creatinine, creatine and uric acid respectively. An optimization of the separation conditions and a comparison of PAD with other amperometric detection modes is also shown. Finally, analysis of a real urine sample is presented with validation of creatinine concentrations using a clinical assay kit based on the Jaffé reaction.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Direct detection of renal function markers using microchip CE with pulsed electrochemical detection

Garcı́a

Henry

2004

Analyst

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“…One of the widely accepted ways to determine creatinine is the Jaffe method, which utilizes picric acid for the Jaffe Reaction to test for creatinine [ 9 ]. It forms a coloured bright orange-red complex that can be measured using spectroscopy.…”

Section: Introductonmentioning

confidence: 99%

Development of a method for optical monitoring of creatinine in the spent dialysate

Tomson

Uhlin²,

Holmar³

et al. 2011

Estonian J. Eng.

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The aim of the study was to develop a method suitable for the estimation of the amount of creatinine, removed during dialysis through UV-absorbance. Sixteen uremic patients, seven females and nine males, on chronic thrice-weekly hemodialysis were included in the study. Doublebeam spectrophotometer was used for the determination of UV-absorbance in the collected spent dialysate samples. Due to differences in independent variables, two multi-wavelength models (m1 and m2) were developed using stepwise regression, utilizing creatinine values from the laboratory as the dependent parameter. The coefficient of determination, R 2 was 0.8690 for the first and 0.9103 for the second model. The systematic error, estimated as BIAS, was zero for both models compared to the creatinine values from the laboratory. The standard errors were 10.06 µmol/l and 15.24 µmol/l for m1 and m2, respectively. The average reduction ratio (RR) from creatinine blood values was 59.8 ± 5.4% (N = 50), average RR from m1 was 63.7 ± 7.3% (N = 50) and average RR from m2 was 64.8 ± 6.4% (N = 48). In summary, the amount of creatinine removed as well as the reduction ratio of creatinine during dialysis can be estimated with UV-absorbance technique.

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Janovsky Reaction

2010

Comprehensive Organic Name Reactions and Reagents

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The reaction between aromatics containing multinitro groups and aldehydes or ketones with an activated α‐proton in the presence of a strong base to form coloring molecules, for detection of both carbonyl compounds and nitroaromatics is generally referred to as the Janovsky reaction and the product formed is known as Janovsky adduct. It has been reported that substituents other than the nitro groups on the aromatic rings may influence the reactivity of substrates or alter the resonance of the nitro groups in the quinoid ion. On the other hand, the relative location of the nitro groups also affect the outcome of this reaction. The addition of enolate to the nitroaromatics at low temperature is primarily through an O ‐attack and at high temperature the O ‐attack intermediate gradually changes to the anion originated from C ‐attack. This reaction has been further modified to measure 3,5‐dinitrobenzamide using ammonium hydroxide as base. This reaction has wide applications in forensic toxicology, agriculture ,and pharmaceutical testing.

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Application and evaluation of a new cold-stable kinetic Jaffé reagent to the Hitachi 747 for the determination of serum creatinine

Cited by 7 publications

References 11 publications

Direct detection of renal function markers using microchip CE with pulsed electrochemical detection

Direct detection of renal function markers using microchip CE with pulsed electrochemical detection

Development of a method for optical monitoring of creatinine in the spent dialysate

Janovsky Reaction

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