Application and prospects of high-throughput screening for <i>in vitro </i>neurogenesis

Zhang, Shuyuan; Zhao, Juan; Ni, Junjun; Li, Hui; Quan, Zhenzhen; Qing, Hong

doi:10.4252/wjsc.v14.i6.393

Cited by 2 publications

(2 citation statements)

References 276 publications

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“…Moreover, when combined with artificial intelligence, automated organoid culture processes have the capacity to revolutionize disease modeling and significantly streamline drug discovery for clinical trials. This integration promises to yield valuable insights and accelerate progress in the quest for more effective therapies [108][109][110][111]. This integration promises to yield valuable insights and accelerate progress in the quest for more effective therapies [109][110][111].…”

Section: Major Limitations Of Current Human Brain Mps Models For Biom...mentioning

confidence: 98%

Basic models to advanced systems: harnessing the power of organoids-based microphysiological models of the human brain

Boylin,

Aquino,

Purdon

et al. 2024

Biofabrication

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Understanding the complexities of the human brain's function in health and disease is a formidable challenge in neuroscience. While traditional models like animals offer valuable insights, they often fall short in accurately mirroring human biology and drug responses. Moreover, recent legislation has underscored the need for more predictive models that more accurately represent human physiology. To address this requirement, human-derived cell cultures have emerged as a crucial alternative for biomedical research. However, traditional static cell culture models lack the dynamic tissue microenvironment that governs human tissue function. Advanced in vitro systems, such as organoids and Microphysiological systems (MPS), bridge this gap by offering more accurate representations of human biology. Organoids, which are three-dimensional miniaturized organ-like structures derived from stem cells, exhibit physiological responses akin to native tissues, but lack essential tissue-specific components such as functional vascular structures and immune cells. Recent endeavors have focused on incorporating endothelial cells and immune cells into organoids to enhance vascularization, maturation, and disease modeling. MPS, including organ-on-chip technologies, integrate diverse cell types and vascularization under dynamic culture conditions, revolutionizing brain research by bridging the gap between in vitro and in vivo models. In this review, we delve into the evolution of MPS, with a particular focus on highlighting the significance of vascularization in enhancing the viability, functionality, and disease modeling potential of organoids. By examining the interplay of vasculature and neuronal cells within organoids, we can uncover novel therapeutic targets and gain valuable insights into disease mechanisms, offering the promise of significant advancements in neuroscience and improved patient outcomes.

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Section: Major Limitations Of Current Human Brain Mps Models For Biom...mentioning

confidence: 98%

Basic models to advanced systems: harnessing the power of organoids-based microphysiological models of the human brain

Boylin,

Aquino,

Purdon

et al. 2024

Biofabrication

View full text Add to dashboard Cite

show abstract

“…neurotransmitters, growth factors, metabolites) needed for the regulation of cells fate at different stages of their development; (iii) establishment of concentration gradients of molecules in transwell systems or within the microfluidic platforms for the controlled proliferation, differentiation and migration of newly-born cells; (iv) maintaining the long-lived multicellular ensembles recapitulating the whole cycle of neurogenesis in vitro with the permanent monitoring of cells expression pattern, their proliferation and differentiation capacity. 239 – 243 …”

Section: Development Of a Changeable Brain: 4d Neurogenic Niche In Vi...mentioning

confidence: 99%

Current progress and challenges in the development of brain tissue models: How to grow up the changeable brain in vitro?

Salmina,

Alexandrova,

Averchuk

et al. 2024

J Tissue Eng

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In vitro modeling of brain tissue is a promising but not yet resolved problem in modern neurobiology and neuropharmacology. Complexity of the brain structure and diversity of cell-to-cell communication in (patho)physiological conditions make this task almost unachievable. However, establishment of novel in vitro brain models would ultimately lead to better understanding of development-associated or experience-driven brain plasticity, designing efficient approaches to restore aberrant brain functioning. The main goal of this review is to summarize the available data on methodological approaches that are currently in use, and to identify the most prospective trends in development of neurovascular unit, blood-brain barrier, blood-cerebrospinal fluid barrier, and neurogenic niche in vitro models. The manuscript focuses on the regulation of adult neurogenesis, cerebral microcirculation and fluids dynamics that should be reproduced in the in vitro 4D models to mimic brain development and its alterations in brain pathology. We discuss approaches that are critical for studying brain plasticity, deciphering the individual person-specific trajectory of brain development and aging, and testing new drug candidates in the in vitro models.

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Application and prospects of high-throughput screening for in vitro neurogenesis

Cited by 2 publications

References 276 publications

Basic models to advanced systems: harnessing the power of organoids-based microphysiological models of the human brain

Basic models to advanced systems: harnessing the power of organoids-based microphysiological models of the human brain

Current progress and challenges in the development of brain tissue models: How to grow up the changeable brain in vitro?

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