Owing to the increasing applications and popularity of grouting reinforcement technology for water control in mine floors, its control effect is becoming increasingly concerning. However, the lack of an effective evaluation method for the mine floor composite limestone aquifers under different treatment modes can lead to blindness and randomness in grouting engineering. Thus, based on engineering practices, we analyzed the prevention and control effect of water inrush from the working face floor under different grouting reinforcement and transformation modes. Moreover, we established a sub-model of “pipe pile” that was reinforced by cross-layer drilling grouting and a sub-model of “water-resistant key stratum” that was reinforced by regional treatment grouting, while we also simulated and analyzed the concrete function of the structural models formed by the two grouting modes. Different microseismic monitoring arrays were used to monitor and evaluate the grouting reinforcement effect of the working face floor. The monitoring results revealed noticeable differences in the microseismic characteristics of the floor limestone during the mining process under different treatment modes. This provides a basis for evaluating the grouting reinforcement effect.