Application and Theory of Unidimensional Multiple Chromatography.

Abstract

lowed by decreases with preboiling time. Hence, furfural appeared to react through an intermediate, possibly a ring opening. Such an open-ring structure was suggested by Wolfrom, Schuetz, and Cavalieri (9). Teunissen (8) investigated the scission of the furan ring under the influence of acidic reagents.He suggested the intramolecular migration of certain atoms prior to a breakdown into levulinic and formic acids.One important qualitative feature of the reactions with OPD was that the fluorescent solutions obtained from numerous sugars possessed approximately the same excitation and fluorescence wavelength maxima (360 and 460 mg). This fact suggests the possi-

Intracellular Distribution and Characterization of the Lipids of Streptococcus faecalis (ATCC 9790)*

“…Paper chromatography of the sugars was carried out by the ascending technique using butanol-pyridinewater (45:25:40, v/v/v) and ethyl acetate-pyridinewater (30:21:9, v/v/v). Multiple development (Thoma, 1963) was used to obtain complete resolution of glucose and galactose. The periodate-Schiff procedure of Buchanan et al (1950) was used for detection of vicinal hydroxyls, and the aniline-hydrogen phthalate reagent (Partridge, 1949) was used for detection of reducing sugars.…”

Identification of biotransformation products from 2,4-dinitrotoluene

“…To resolve these compounds, the TLC plate was developed, removed from the solvent, dried, and developed again in the same solvent system. This process of multiple development (12) was repeated as many times as necessary to achieve separation between areas of almost equal Rf values. Gas chromatography/mass spectrometry (GC/MS).…”

Selection of a Chromatographic Solvent.

“…An example of a plot used for evaluating the chromatographic parameters for a series of structurally related compounds is illustrated in Figure 5. Although the data in Figure 5 were collected for unionized carbohydrate molecules, the data of Burma and Banerjee (1) for amino acids in the water-propanol system also lend to linear lines intersecting at a common point (38). The only exception (38) occurred when methionine was used as one of a pair of amino acids.…”