Application Note: μPolar - An Interactive 2D Visualization Tool for Time-Series Microscopic Images

DetecDiv, a generalist deep-learning platform for automated cell division tracking and survival analysis

“…The initial development of microfluidic systems for RLS assays has partially alleviated this problem by allowing continuous observation of individual cell divisions and relevant fluorescent cellular markers under the microscope from birth to death (Lee et al 2012;Xie et al 2012;Fehrmann et al 2013). Recent efforts further increased data acquisition throughput (Jo et al 2015;Liu, Young, and Acar 2015) and attempted to automate data analysis Ghafari, Mailman, and Qin 2021). Yet, retrieving individual cellular lifespans from large sets of image sequences so far remained an insurmountable bottleneck to characterize senescence entry quantitatively or to screen large numbers of mutants and environmental conditions.…”