Application of 1H-NMR combined with qRT-PCR technology in the exploration of rosmarinic acid biosynthesis in hair roots of Salvia miltiorrhiza Bunge and Salvia castanea f. tomentosa Stib

Hou, Zhuoni; Li, Yuanyuan; Feng, Shengyu; Chen, Jipeng; Zhang, Xiaodan; Xu, Ling; Yang, Dongfeng; Liang, Zongsuo

doi:10.1007/s00425-020-03506-y

Cited by 11 publications

(5 citation statements)

References 46 publications

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“…Salvia miltiorrhiza Bunge is a popular Chinese medicinal herb. Methyl jasmonate (MeJA) enhanced the synthesis of RA in S. miltiorrhiza through regulation of the transcription factor gene SmMYB2 and secondary metabolism-related genes [tyrosine aminotransferase ( TAT ) and HPPR ] [ 38 , 39 ]. Equal amounts of cellulase A and protamex mixture exhibited maximum effectiveness in extracting RA at 28.23 mg/g [ 40 ].…”

Section: Culture Techniques Extraction Processes and Anti-tumor Appli...mentioning

confidence: 99%

Rosmarinic Acid and Related Dietary Supplements: Potential Applications in the Prevention and Treatment of Cancer

Zhao

Jin

et al. 2022

Biomolecules

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Cancer constitutes a severe threat to human health and quality of life and is one of the most significant causes of morbidity and mortality worldwide. Natural dietary products have drawn substantial attention in cancer treatment and prevention due to their availability and absence of toxicity. Rosmarinic acid (RA) is known for its excellent antioxidant properties and is safe and effective in preventing and inhibiting tumors. This review summarizes recent publications on culture techniques, extraction processes, and anti-tumor applications of RA-enriched dietary supplements. We discuss techniques to improve RA bioavailability and provide a mechanistic discussion of RA regarding tumor prevention, treatment, and adjuvant therapy. RA exhibits anticancer activity by regulating oxidative stress, chronic inflammation, cell cycle, apoptosis, and metastasis. These data suggest that daily use of RA-enriched dietary supplements can contribute to tumor prevention and treatment. RA has the potential for application in anti-tumor drug development.

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Section: Culture Techniques Extraction Processes and Anti-tumor Appli...mentioning

confidence: 99%

Rosmarinic Acid and Related Dietary Supplements: Potential Applications in the Prevention and Treatment of Cancer

Zhao

Jin

et al. 2022

Biomolecules

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“…Quantitative Real-Time PCR. The qRT-PCR was performed using a ChamQ Green I SYBR qPCR Master Mix (Q711) qPCR Kit (Vazyme, Nanjing, China) with the Quant-Studio™ 6 Flex Real-Time PCR System (Applied Biosystems, Thermo, Singapore) under the following conditions [15]: 95 °C for 30s, 40 cycles of 95 °C for 5s, and 55 °C for 30 s, followed by 95 °C for 15 s, 60 °C for 1 min, and 95 °C for 15 s to obtain melt…”

Section: 24mentioning

confidence: 99%

Cloning and Characterization of the Gene Encoding HMGS Synthase in Polygonatum sibiricum

Jiang

Wang

et al. 2022

BioMed Research International

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The saponins of Polygonatum sibiricum had many pharmacological activities such as antitumor, antioxidation, and blood sugar lowering, which were synthesized by two pathways: mevalonate (MVA) and methylerythritol phosphate (MEP). 3-Hydroxy-3-methylglutaryl coenzyme A synthase (HMGS) was the key enzyme in the MVA synthesis pathway, and its expression level may affect the accumulation of saponins which were the main active ingredients of P. sibiricum. In this study, we successfully cloned HMGS1 and HMGS2 from P. sibiricum and their sequence similarity was 93.71% with 89 different sites. The multiple sequence alignment results indicated that the N-terminal sequences of HMGS were conserved. Phylogenetic analysis showed that P. sibiricum, A. officinalis, N. tazetta, D. nobile, and other relatives had a common evolutionary ancestor. The expression levels of both HMGSs and the total saponin content in different tissues revealed that HMGS expression in rhizomes was positively correlated with total saponin content. Further study of the abiotic stress effect of Methyl Jasmonate (MeJA) demonstrated that the expression of HMGS1 and HMGS2 genes was induced by MeJA, peaked at 24 h, and fell by 48 h. Our present findings would provide a blueprint for future studies of HMGS and its role in triterpenoid biosynthesis in P. sibiricum.

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“…Compound 10 is a phenolic acid; Jindra et al [28] had reported the presence of compound 10 in Papavar somniferum plants. Furthermore, Hou et al [29] had identified this compound as a metabolite in the metabolic pathways of Salvia miltiorrhiza Bunge (Chinese medicinal plant) and Salvia castanea f. tomentosa Stib, where they used methyl jasmoate (phytohormone) as an inducer to synthesize phenolic acids in the hairy roots of S. miltiorrhiza Bange and S. castanea f. tomentosa Stib and quantified compound 10 using 1 H-NMR. They found the chemical shifts of compound 10 around δ 6.00, δ 7.00, and δ 4.00, which are in line with the present study.…”

Section: Putative Compounds Identified By Nmrmentioning

confidence: 99%

“…S. castanea f. tomentosa Stib possess similar pharmacological activities to S. miltiorrhiza Bange. Though no anti-diabetic activity has been reported for the compound 10, a significant quantities of this compound was reported in methyl jasmoate treated hairy roots of S. miltiorrhiza Bange and S. castanea f. tomentosa Stib [29,56]. On the other hand, phenolic acids are highly potential for various biological activities, including antioxidant and antimicrobial activities [57].…”

Section: Putative Compounds Identified By Nmrmentioning

confidence: 99%

GC-MS- and NMR-Based Metabolomics and Molecular Docking Reveal the Potential Alpha-Glucosidase Inhibitors from Psychotria malayana Jack Leaves

et al. 2021

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Psychotria malayana Jack leaf, known in Indonesia as “daun salung”, is traditionally used for the treatment of diabetes and other diseases. Despite its potential, the phytochemical study related to its anti-diabetic activity is still lacking. Thus, this study aimed to identify putative inhibitors of α-glucosidase, a prominent enzyme contributing to diabetes type 2 in P. malayana leaf extract using gas chromatography-mass spectrometry (GC-MS)- and nuclear magnetic resonance (NMR)-based metabolomics, and to investigate the molecular interaction between those inhibitors and the enzyme through in silico approach. Twenty samples were extracted with different solvent ratios of methanol–water (0, 25, 50, 75, and 100% v/v). All extracts were tested on the alpha-glucosidase inhibition (AGI) assay and analyzed using GC-MS and NMR. Multivariate data analysis through a partial least square (PLS) and orthogonal partial square (OPLS) models were developed in order to correlate the metabolite profile and the bioactivity leading to the annotation of the putative bioactive compounds in the plant extracts. A total of ten putative bioactive compounds were identified and some of them reported in this plant for the first time, namely 1,3,5-benzenetriol (1); palmitic acid (2); cholesta-7,9(11)-diene-3-ol (3); 1-monopalmitin (4); β-tocopherol (5); α-tocopherol (6); 24-epicampesterol (7); stigmast-5-ene (8); 4-hydroxyphenylpyruvic acid (10); and glutamine (11). For the evaluation of the potential binding modes between the inhibitors and protein, the in silico study via molecular docking was performed where the crystal structure of Saccharomyces cerevisiae isomaltase (PDB code: 3A4A) was used. Ten amino acid residues, namely ASP352, HIE351, GLN182, ARG442, ASH215, SER311, ARG213, GLH277, GLN279, and PRO312 established hydrogen bond in the docked complex, as well as hydrophobic interaction of other amino acid residues with the putative compounds. The α-glucosidase inhibitors showed moderate to high binding affinities (−5.5 to −9.4 kcal/mol) towards the active site of the enzymatic protein, where compounds 3, 5, and 8 showed higher binding affinity compared to both quercetin and control ligand.

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Application of 1H-NMR combined with qRT-PCR technology in the exploration of rosmarinic acid biosynthesis in hair roots of Salvia miltiorrhiza Bunge and Salvia castanea f. tomentosa Stib

Cited by 11 publications

References 46 publications

Rosmarinic Acid and Related Dietary Supplements: Potential Applications in the Prevention and Treatment of Cancer

Rosmarinic Acid and Related Dietary Supplements: Potential Applications in the Prevention and Treatment of Cancer

Cloning and Characterization of the Gene Encoding HMGS Synthase in Polygonatum sibiricum

GC-MS- and NMR-Based Metabolomics and Molecular Docking Reveal the Potential Alpha-Glucosidase Inhibitors from Psychotria malayana Jack Leaves

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