Application of 2H stable isotope labelling methodology and ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the metabolite identification of dehydroandrographolide in rats

Bai, Pengpeng; Niu, Kaixia; Huo, Zhipeng; Feng, Xinchi; Qiu, Feng

doi:10.1007/s44211-022-00129-z

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…Hanh et al used nuclear magnetic resonance, MS, and other spectral methods to conduct a chemical analysis of A. paniculata , and isolated and identified nine compounds; of these dehydroandrographolide (DA) was considered as one of the important diterpenoids (Hanh et al, 2020). Therefore, the metabolic process of DA was studied using UHPLC‐Q‐TOF‐MS/MS, and 35 metabolites were identified in rat urine, bile, plasma, and feces samples (Bai et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

A comprehensive study of Chuanwang Xiaoyan capsule based on characterization of chemical constituents in vitro and in vivo using ultra‐high‐performance liquid chromatography‐quadrupole‐Exactive Orbitrap mass spectrometry and pharmacokinetic study of multiple components in rats using ultra‐high‐performance liquid chromatography‐triple quadrupole‐tandem mass spectrometry

Wang,

Gao

et al. 2024

Biomedical Chromatography

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Chuanwang Xiaoyan (CWXY) capsule is primarily used to treat a variety of acute and chronic inflammations, including acute and chronic pharyngitis and tonsillitis. However, a systematic study of its chemical constituents is still not available. This study evaluated the chemical constituents in vitro and metabolite profiles in vivo of CWXY using ultra‐high‐performance liquid chromatography (UHPLC) coupled with Q‐Exactive Orbitrap mass spectrometry, and the pharmacokinetic behaviors of the nine main components in rats were detected using ultra‐high‐performance liquid chromatography‐triple quadrupole‐mass spectrometry (UPLC‐QQQ‐MS/MS). A total of 92 chemical constituents in CWXY were preliminarily identified in vitro. After oral administration to rats, 56 prototype components and 128 metabolites of CWXY were detected in the biological samples of rat plasma, urine, bile, and feces. Of these prototype components and metabolites, seven new compounds, namely M15, M16, M25, M30, M31, M71, and M128, were detected for the first time. The quantitation method of nine components in rat plasma was developed using a pharmacokinetic study. To the best of our knowledge, this study was the first to investigate the pharmacokinetic behavior of triumbelletin.

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Section: Introductionmentioning

confidence: 99%

A comprehensive study of Chuanwang Xiaoyan capsule based on characterization of chemical constituents in vitro and in vivo using ultra‐high‐performance liquid chromatography‐quadrupole‐Exactive Orbitrap mass spectrometry and pharmacokinetic study of multiple components in rats using ultra‐high‐performance liquid chromatography‐triple quadrupole‐tandem mass spectrometry

Wang,

Gao

et al. 2024

Biomedical Chromatography

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Small molecule metabolites: discovery of biomarkers and therapeutic targets

Qiu

Cai

et al. 2023

Sig Transduct Target Ther

234

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Metabolic abnormalities lead to the dysfunction of metabolic pathways and metabolite accumulation or deficiency which is well-recognized hallmarks of diseases. Metabolite signatures that have close proximity to subject’s phenotypic informative dimension, are useful for predicting diagnosis and prognosis of diseases as well as monitoring treatments. The lack of early biomarkers could lead to poor diagnosis and serious outcomes. Therefore, noninvasive diagnosis and monitoring methods with high specificity and selectivity are desperately needed. Small molecule metabolites-based metabolomics has become a specialized tool for metabolic biomarker and pathway analysis, for revealing possible mechanisms of human various diseases and deciphering therapeutic potentials. It could help identify functional biomarkers related to phenotypic variation and delineate biochemical pathways changes as early indicators of pathological dysfunction and damage prior to disease development. Recently, scientists have established a large number of metabolic profiles to reveal the underlying mechanisms and metabolic networks for therapeutic target exploration in biomedicine. This review summarized the metabolic analysis on the potential value of small-molecule candidate metabolites as biomarkers with clinical events, which may lead to better diagnosis, prognosis, drug screening and treatment. We also discuss challenges that need to be addressed to fuel the next wave of breakthroughs.

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Development of Cryogenic HPLC for Wide Range Low Temperatures Using Liquefied Propane as Mobile Phase and Its Application to Hydrogen/Deuterium Compounds Separation

HATTORI,

IWASE,

KITAGAWA

et al. 2024

Chromatography

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Application of 2H stable isotope labelling methodology and ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the metabolite identification of dehydroandrographolide in rats

Cited by 3 publications

References 22 publications

Small molecule metabolites: discovery of biomarkers and therapeutic targets

Development of Cryogenic HPLC for Wide Range Low Temperatures Using Liquefied Propane as Mobile Phase and Its Application to Hydrogen/Deuterium Compounds Separation

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Application of 2H stable isotope labelling methodology and ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the metabolite identification of dehydroandrographolide in rats

Cited by 3 publications

References 22 publications

Small molecule metabolites: discovery of biomarkers and therapeutic targets

Development of Cryogenic HPLC for Wide Range Low Temperatures Using Liquefied Propane as Mobile Phase and Its Application to Hydrogen/Deuterium Compounds Separation

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Product

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Application of 2H stable isotope labelling methodology and ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the metabolite identification of dehydroandrographolide in rats