Application of 3-(2-furoyl)quinoline-2-carbaldehyde as a fluorogenic reagent for the analysis of primary amines by liquid chromatography with laser-induced fluorescence detection

Beale, Stephen C.; Hsieh, You‐Zung; Wiesler, Donald; Novotný, Miloš V.

doi:10.1016/s0021-9673(00)97002-x

Cited by 56 publications

(24 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This compound generates stable fluorescent isoindol derivatives when it reacts with primary amines in the presence of nucleophilic agents, such as KCN [18]. The derivatization reaction was performed inside the capillary.…”

Section: Ce Proceduresmentioning

confidence: 99%

Development of a method for quantitative analysis of the major whey proteins by capillary electrophoresis with on‐capillary derivatization and laser‐induced fluorescence detection

Veledo¹,

Frutos²,

Diez-Masa³

2005

J of Separation Science

View full text Add to dashboard Cite

The main whey proteins have been derivatized on-capillary with 3-(2-furoyl)quinoline-2-carboxaldehyde (FQ) and analyzed using a laboratory-made capillary electrophoresis apparatus provided with a laser-induced fluorescence detector. Several parameters controlling on-capillary derivatization of proteins, including pH, mixing time, reaction time, concentration of the reagents (potassium cyanide and FQ), and reaction temperature, were optimized. Coefficient variations were lower than 1% for migration time and 7% for peak height. Assay detection limits for the different proteins were in the range 5 nM to 10 nM. The method developed was applied to the separation of the major whey proteins in a laboratory-made cheese whey and in an infant food formulated with milk. In addition, the beta-LG content of these samples was quantitated. The results showed good agreement with those given by an RP-HPLC method and with those reported in the literature.

show abstract

Section: Ce Proceduresmentioning

confidence: 99%

Development of a method for quantitative analysis of the major whey proteins by capillary electrophoresis with on‐capillary derivatization and laser‐induced fluorescence detection

Veledo¹,

Frutos²,

Diez-Masa³

2005

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…FQ has been used for amino acid analysis in HPLC since the 1990s [29]. Initially it was used to detect picomole amounts of insulin in very dilute solutions using CE-LIF [30].…”

Section: Chemical Reaction Studiesmentioning

confidence: 99%

Laser‐induced fluorescence detection schemes for the analysis of proteins and peptides using capillary electrophoresis

et al. 2005

View full text Add to dashboard Cite

Over the past few years, a large number of studies have been prepared that describe the analysis of peptides and proteins using capillary electrophoresis (CE) and laser-induced fluorescence (LIF). These studies have focused on two general goals: (i) development of automatic, selective and quick separation and detection of mixtures of peptides or proteins; (ii) generation of new methods of quantitation for very low concentrations (nm and subnanomolar) of peptides. These two goals are attained with the use of covalent labelling reactions using a variety of dyes that can be readily excited by the radiation from a commonly available laser or via the use of noncovalent labelling (immunoassay using a labelled antibody or antigen or noncovalent dye interactions). In this review article, we summarize the works which were performed for protein and peptide analysis via CE-LIF.

show abstract

“…OPA, NDA, CNBQCA and BQCA products are detected with He-Cd laser. The agent 3-(2-furoyl)-quinoline-2-carbaldehyde (FQCA) produces fluorescent isoindoles with an excitation maximum near the 488-nm induced by an Ar-ion laser [38]. Fluorescein isothiocyanate (FITC) is the derivatizing agent with higher quantum efficiency compared with the previously mentioned agents.…”

Section: Detection Techniques Coupled To Cementioning

confidence: 99%

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Páez

Hernández

2001

Biopharm & Drug Disp

View full text Add to dashboard Cite

Capillary electrophoresis (CE) is a high-efficiency analytical technique that has had a great impact as a tool in biomedical research, clinical and forensic practice in the last ten years. Only in one of the applications, the DNA analysis, it has had an explosive exponential growth in the last few years. This impact is expressed in an enormous amount of CE articles and many reviews. The CE advantages with respect to other analytical techniques: the required very small sample volume, rapid analysis, great resolution power and low costs, have made this technique ideal for the analysis of a numerous endogenous and exogenous substances present in biological fluids. The different modes of CE have been coupled to different detection techniques such as UV-absorbance, electrochemical, mass spectrometry and laser-induced fluorescence detection (LIFD) to detect different nature and molecular size separated analytes. This review focuses mostly on the applications of CE-LIFD, to measure drugs and endogenous neuroactive substances such as amino acids and monoamines, especially in microdialysis samples from experimental animals and humans. CE-LIFD trends are discussed: automated faster analysis with capillary array systems, resolution power improvement, higher detection sensitivity, and CE systems miniaturization for extremely small sample volume, in order to make CE easier and affordable to the lab bench or the clinical bed.

show abstract

Application of 3-(2-furoyl)quinoline-2-carbaldehyde as a fluorogenic reagent for the analysis of primary amines by liquid chromatography with laser-induced fluorescence detection

Cited by 56 publications

References 23 publications

Development of a method for quantitative analysis of the major whey proteins by capillary electrophoresis with on‐capillary derivatization and laser‐induced fluorescence detection

Development of a method for quantitative analysis of the major whey proteins by capillary electrophoresis with on‐capillary derivatization and laser‐induced fluorescence detection

Laser‐induced fluorescence detection schemes for the analysis of proteins and peptides using capillary electrophoresis

Biomedical applications of capillary electrophoresis with laser‐induced fluorescence detection

Contact Info

Product

Resources

About