Application of a cation-exchange reaction of CuS nanoparticles and fluorescent copper nanoparticles in a DNA biosensor

Abstract

A novel detection method based on cation-exchange of CuS nanoparticles combined with poly T-templated fluorescent Cu nanoparticles was developed and applied to the exonuclease III catalyst DNA biosensor.

Tuning DNA–nanoparticle conjugate properties allows modulation of nuclease activity

“…Exonuclease III (ExoIII) was chosen due to its widespread use in biological sensing applications, and in particular, systems co-employing nanomaterials and enzymes. [32][33][34][35] ExoIII is a nonprocessive enzyme that acts on double-stranded DNA (dsDNA), where it cleaves mononucleotides from the 3′-hydroxyl terminus of one strand, leaving the other strand intact. 36,37 The preferred substrates are blunt or recessed 3′-termini of dsDNA, but the enzyme can also act at nicks or abasic sites.…”

Interplay between Chemical Transformations and Atomic Structure in Nanocrystals and Nanoclusters

“…Chemical transformation of nanocrystals and nanoclusters (CTNs) are the reactions that are performed on the first-generation (as-synthesized) nanocolloids that effectively modify their atomic structure, chemical composition, particle size, and morphology. , The motivations behind CTN research are to understand how and if the mechanisms for these reactions differ at the nanoscale compared to bulk, to expand the scope of existing functional materials, and to explore new metastable phases that are inaccessible from a conventional synthetic approach . CTNs can produce new nanomaterial forms with novel properties that are useful in applications, such as catalysis, − biosensing, , optical devices, solar cells, and transistors, , significantly fostering the development of the materials engineering for device integration . These examples clearly show the power of chemical transformation approach to finely tune the properties of nanomaterials to make them suitable for specific application.…”

The effects of thioacetamide/copper molar ratio and reaction time on the phase evolution, morphology, optical, and photocatalytic properties of the nanosheets‐based flower‐like copper sulfide

“…Self‐organization combines the nanostructures into new 3D nanostructured materials . The chalcogenide semiconductors have extensive applications in various fields such as cancer therapy, solar cell, DNA biosensor, Photo‐acoustic agent, antibacterial, optical and photocatalytic, optoelectronics, magnesium secondary batteries, and artificial photosynthesis . The band gap of the semiconductor, which plays a significant role in the photocatalytic properties of copper sulfide nanoparticles, can be controlled according to the stoichiometry of Cu:S and the type of synthesis method.…”