Application of a Fast Separation Method for Anti-diabetics in Pharmaceuticals Using Monolithic Column: Comparative Study With Silica Based C-18 Particle Packed Column

Hemdan, A.; Abdelaziz, Omar Y.

doi:10.1093/chromsci/bmy009

Cited by 3 publications

(1 citation statement)

References 18 publications

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“…On the other hand, the highly porous silica rods with the revolutionary double pore structure of the monolithic HPLC columns help cut down the run time [5,6]. The two types of voids in the monolithic column are mesopores and macropores.…”

Section: Introductionmentioning

confidence: 99%

Eco-Friendly Separation of Antihyperlipidemic Combination Using UHPLC Particle-Packed and Monolithic Columns by Applying Green Analytical Chemistry Principles

Al‐Tannak

Hemdan

2021

Separations

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Efficient separation of pharmaceuticals and metabolites with the adequate resolution is a key factor in choosing the most suitable chromatographic method. For quality control, the analysis time is a key factor, especially in pharmacokinetic studies. High back pressure is considered as one of the most important factors in chromatography’s flow control, especially in UHPLC. The separation of the anti-hyperlipidemic mixtures was carried out using two columns: a column silica-based particle packed UHPLC and a monolithic column. The systematic suitability of the two columns was compared for the separation of Fenofibrate, its active metabolite, Fenofibric acid and Pravastatin using Atorvastatin as an internal standard. Separation on both columns was obtained using ethanol: buffer potassium dihydrogen orthophosphate pH = 3 (adjusted with orthophosphoric acid) (75:25 v/v) as mobile phase and flow rate 0.8 mL/min. The analytes’ peak detection was achieved by using a PDA detector at 287 nm, 214 nm, 236 nm, and 250 nm for Fenofibrate, Fenofibric acid, Pravastatin, and Atorvastatin, respectively. Reduction of back-pressure was achieved with the monolithic column, where the analytes could be completely separated in less than 1.5 min at a flow rate of 5 mL/min. The principles of Green Analytical Chemistry (GAC) were followed throughout the developed method using environmentally safe solvents.

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Section: Introductionmentioning

confidence: 99%

Eco-Friendly Separation of Antihyperlipidemic Combination Using UHPLC Particle-Packed and Monolithic Columns by Applying Green Analytical Chemistry Principles

Al‐Tannak

Hemdan

2021

Separations

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Development and Validation of High-Performance Liquid Chromatography Method for the Quantification of Remdesivir in Intravenous Dosage Form

Jitta

Salwa

Kumar

et al. 2021

ASSAY and Drug Development Technologies

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Eco-friendly HPLC method by using response surface design to measure a combination of three antidiabetic drugs

Al-Tannak,

Hemdan

2023

Reviews in Analytical Chemistry

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In recent years, there has been a significant increase in the application of green chemistry principles in analytical chemistry. One of the key factors affecting the environmental sustainability of analytical methods is the choice of solvent. In this particular study, a two-factor, three-level response surface design was employed to develop an environmentally friendly chromatographic technique for a mixture of metformin, glimepiride, and pioglitazone. Ethanol, which is known to be harmless to the environment, was chosen as the organic modifier in the mobile phase. The separation of the mixture was achieved using a phosphate buffer solution (pH 3) mixed with ethanol (30:70 v/v). The linearity of the developed method covered a concentration of metformin of 10–120 µg·mL−1 with a correlation coefficient of 0.9998, glimepiride of 0.1–20 µg·mL−1 with a correlation coefficient of 0.9997, and pioglitazone of 1–50 µg·mL−1 with a correlation coefficient of 0.9999. To evaluate the environmental friendliness of the developed method, two assessment tools were employed: The Analytical GREEnness metric and Green Analytical Procedure Index. The results revealed that the developed method performed exceptionally well in terms of its eco-friendliness. Furthermore, the developed method was compared to other reported methods in terms of both accuracy and environmental sustainability. The results demonstrated that the developed method serves as an excellent alternative to well-established techniques for the separation and quantification of the analyzed mixture. Overall, this study highlights the importance of integrating green chemistry principles into analytical chemistry practices.

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Application of a Fast Separation Method for Anti-diabetics in Pharmaceuticals Using Monolithic Column: Comparative Study With Silica Based C-18 Particle Packed Column

Cited by 3 publications

References 18 publications

Eco-Friendly Separation of Antihyperlipidemic Combination Using UHPLC Particle-Packed and Monolithic Columns by Applying Green Analytical Chemistry Principles

Eco-Friendly Separation of Antihyperlipidemic Combination Using UHPLC Particle-Packed and Monolithic Columns by Applying Green Analytical Chemistry Principles

Development and Validation of High-Performance Liquid Chromatography Method for the Quantification of Remdesivir in Intravenous Dosage Form

Eco-friendly HPLC method by using response surface design to measure a combination of three antidiabetic drugs

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