Application of a horizontal DS chamber to planar electrochromatography

Dzido, Tadeusz H.; Majewski, Rafał; Polak, Beata; Gołkiewicz, W.; Soczewiński, Edward

doi:10.1556/jpc.16.2003.3.1

Cited by 31 publications

(11 citation statements)

References 8 publications

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“…This effect was reduced to some extent by application of an appropriate buffer concentration in the mobile phase, [11] or a special strip of counter plate fixed to the adsorbent layer between solvent entry position and start line of the sample. [13] However, these attempts were not satisfactory and reproducibility of retention was still worse than in a TLC mode.…”

Section: Introductionmentioning

confidence: 99%

Planar Electrochromatography in a Closed System under Pressure—Pressurized Planar Electrochromatography

Dzido

Płocharz

2007

Journal of Liquid Chromatography & Related Technologies

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Section: Introductionmentioning

confidence: 99%

Planar Electrochromatography in a Closed System under Pressure—Pressurized Planar Electrochromatography

Dzido

Płocharz

2007

Journal of Liquid Chromatography & Related Technologies

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“…Another solution was presented in a paper by our group. We have proposed restricted feeding of the chromatographic plate with the mobile phase solution from the reservoir . The plate number obtained in these systems was several times larger than that in conventional TLC.…”

mentioning

confidence: 99%

Apparatus for Pressurized Planar Electrochromatography in a Completely Closed System

2006

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Pressurized planar electrochromatography (PPEC) is the mode which offers much higher separation efficiency in comparison to conventional planar chromatography, including both higher performance and much higher speed of separation. In this paper, we present a new device for performing PPEC in which the whole area of the chromatographic plate is pressurized. Both electrodes (anode and cathode) are washed with the mobile phase during the experiment, which prevents gas bubbles from collection in the region of the electrodes. This device enables directly controlling the flow rate of the mobile phase during the electrochromatography process. Mobile phase control offers the possibility of researching the influence of various properties of the PPEC system on separation efficiency. One important relationship to investigate is plate height vs mobile phase flow rate. This relationship helps to choose the optimal value of the mobile phase flow rate during the separation process. Considerable difference in shape of this relationship is demonstrated for conventional planar chromatography plates and high performance planar chromatography plates. Examples of the influence of some properties of the separating system on flow rate of the mobile phase are demonstrated, such as the buffer concentration in the mobile phase, the pH value of the buffer solution of the mobile phase, the type of chromatographic plate, and the voltage applied to the electrodes.

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“…Apparatus for ion-exchange planar electrochromatography. (1, 2) platform, (3) eluent dish, (4) ion-exchange plate chamber, (5, 6) electrode, (7) collecting tube, (8) T-tube, (9) power supply, (10) peristaltic pump, (11) UV detector, (12) fraction collector, (13) computer, (14) printer.…”

Section: Reagents and Apparatusmentioning

confidence: 99%

“…[10][11][12][13] Dzido et al applied a commercially available horizontal chamber to the planar electrochromatographic separation of a dye mixture on non-polar stationary phases. [14,15] Polak et al used pressurized planar electrochromatography with chiral plates to separate the enantiomers of tryptophan and valine. [16] Tate and Dorsey built an instrument composed of three sections: PEC base, cover grid, and voltage divider box.…”

Section: Introductionmentioning

confidence: 99%

Preparative Separation of Cephalosporin with Ion-Exchange Planar Electrochromatography

Tao

Zheng

Zhao

2009

Journal of Liquid Chromatography & Related Technologies

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A novel device for planar electrochromatography was developed, in which an electric field was applied across a thin layer of strongly acidic ionexchange resin. Three types of cephalosporin -cefamezin, cefotaxime, and cefradine, at preparative amounts, were separated with different mobile phases and applied voltages. Experimental results showed that planar electrochromatography using 0.05 M alanine-acetate solution containing 7.0 M ethanol and 1% Triton X-100 under 200 or 400 V achieved good separation. Critical micelle concentration of Triton X-100 was measured to identify micelle formation in the buffer.

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Application of a horizontal DS chamber to planar electrochromatography

Cited by 31 publications

References 8 publications

Planar Electrochromatography in a Closed System under Pressure—Pressurized Planar Electrochromatography

Planar Electrochromatography in a Closed System under Pressure—Pressurized Planar Electrochromatography

Apparatus for Pressurized Planar Electrochromatography in a Completely Closed System

Preparative Separation of Cephalosporin with Ion-Exchange Planar Electrochromatography

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