Application of a membrane recycle bioreactor for continuous ethanol production

Melzoch, Karel; Rychtera, M.; Markvichov, Nikolay S.; Pospíchalová, Věra; Basařová, G.; Manakov, M. N.

doi:10.1007/bf00180572

Cited by 29 publications

(10 citation statements)

References 14 publications

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“…These com-pounds can be eciently produced using oxygenases that incorporate molecular oxygen directly into the aromatic ring with high stereo-and regio-speci®city; a reaction for which there is no traditional chemistry equivalent. Furthermore, signi®cant advances in the optimization of high cell density fermentations (Choi et al, 1997;Lee, 1996;Makrides, 1996;Ramirez and Bentley, 1995;Yee and Blanch, 1992), the growing number of tools for high level gene expression (Blatny et al, 1997), and in situ product removal schemes (Chang et al, 1992;Melzoch et al, 1991;Molinari et al, 1997;Vick Roy et al, 1982) have removed many of the limitations to the application of biosynthetic routes in the production of value-added chemicals.…”

Section: Introductionmentioning

confidence: 99%

Toluene bioconversion to p‐hydroxybenzoate by fed‐batch cultures of recombinant Pseudomonas putida

Miller

Peretti

2001

Biotech & Bioengineering

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A microbial oxidation process for the production of p-hydroxybenzoate (HBA) from toluene is reported. The oxidation reaction was studied in fed-batch fermentations using a recombinant Pseudomonas putida grown on glutamate as the sole carbon and energy source with salicylate and IPTG induction of tmoABCDE, and pchCF and phbz pathway genes, respectively. An average volumetric HBA productivity of 13.4 mg HBA x L(-1) x h(-1) was obtained under rapid growth conditions (glutamate excess), giving an HBA titer of 132 mg x L(-1) after 9.8 h of fermentation. This corresponded to an average specific HBA productivity of 7.2 microg HBA (mg total protein)(-1) x h(-1). In contrast, maximum HBA titers of 35 mg HBA x L(-1) were achieved in 27 h in comparative studies employing glutumate limited fed-batch cultures. A specific productivity of 4.1 microg HBA (mg total protein)(-1) x h(-1) and volumetric productivity of 1.3 mg HBA x L(-1) x h(-1) were calculated for the growth-rate restricted cultures. The differences in HBA production between the two cultures could be correlated to the levels of specific toluene-4-monooxygenase (T4MO) polypeptides. T4MO catalyzes the rate-limiting step in the pathway. Using experimental data, the half-life value of TmoA was calculated to be approximately 28 h. Assuming linear, monomolecular decay of TmoA, a specific degradation constant of 0.025 x h(-1) was calculated, which placed the stability of recombinant TmoA in the range of relatively stable proteins, even in the absence of co-expression of tmoF, the terminal oxidoreductase subunit of T4MO.

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Section: Introductionmentioning

confidence: 99%

Toluene bioconversion to p‐hydroxybenzoate by fed‐batch cultures of recombinant Pseudomonas putida

Miller

Peretti

2001

Biotech & Bioengineering

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“…Another beneficial effect is that high cell concentrations tend to down-regulate yeast cell growth, resulting in reduced carbon drain for growth. As a consequence, more carbon can be used for ethanol production (Melzoch et al 1991, Palmqvist et al 1998) while a higher cell density, due to the increased number of “working cells”, enables higher volumetric ethanol productivity (Borzani et al, 1993, Navaro 1994, Palmqvist et al 1998, Cardona and Sanchez 2008, Walker 2011). In related work, continuous fermentation using spruce enzymatic hydrolysate showed 4.6 times higher productivity values under high cell density conditions (Palmqvist et al 1998).…”

Section: Discussionmentioning

confidence: 99%

High gravity and high cell density mitigate some of the fermentation inhibitory effects of softwood hydrolysates

2013

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After steam pretreatment of lignocellulosic substrates the fermentation of the biomass derived sugars to ethanol is typically problematic because of both the generally low sugar concentrations that can be supplied and the presence of naturally occurring and process derived inhibitors. As the majority of the inhibitory materials are usually associated with the hemicellulose rich, water soluble component, this fraction was supplemented with glucose to simulate high solids, un-detoxified substrate to see if a high gravity/high cell consistency approach might better cope with inhibition. Several yeast strains were assessed, with the Tembec T1, T2 and Lallemand LYCC 6469 strains showing the greatest ethanol productivity and yield. The addition of supplemental glucose enabled the faster and quantitatively higher removal of hydroxymethylfurfural (HMF). High cell density could provide effective fermentation at high sugar concentrations while enhancing inhibitor reduction. A 77% ethanol yield could be achieved using strain LYCC 6469 after 48 h at high cell density. It was apparent that a high cell density approach improved ethanol production by all of the evaluated yeast strains.

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“…Therefore, we used DSY as a low-cost nitrogen supplement. Phunjumpa et al (2006) also reported that the yeast cell concentration increased 2.5 times of the control medium (without supplementation) when they were cultured in Melzoch media (Melzoch et al, 1991) and tamarind juice supplemented with 1 g l -1 of DSY. However, in our experiment, under the same initial sugar concentration, the maximum viable cell concentrations of SSJ 2 and 5 (with DSY supplementation) were not different from those of SSJ 1 and 4 (without DSY supplementation) (Table 3).…”

Section: Growth Of S Cerevisiae Np 01 In Inoculum Preparation Mediamentioning

confidence: 98%

Repeated-batch ethanol fermentation from sweet sorghum juice by free cells of Saccharomyces cerevisiae NP 01

Ariyajarearnwong¹,

Laopaiboon²,

Jaisil³

et al. 2011

Afr. J. Biotechnol.

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Low-cost inoculum preparation (IP) media for ethanol production were developed. It was found that sweet sorghum juice (SSJ) containing 100 g l -1 of total sugar without nutrient supplement could be used as the low-cost IP medium instead of the typical IP medium or yeast extract malt extract (YM) medium. Ethanol production from the SSJ (total soluble solids of 24 °Bx) by the inoculum of Saccharomyces cerevisiae NP 01 in batch and repeated-batch fermentations was then investigated. The fermentations were carried out under static condition in 500 ml air-locked Erlenmeyer flasks at 30°C and the initial yeast cell concentration was 1×10 8 cells ml , 2.29 ± 0.01 g l -1 h -1 and 0.51 ± 0.02, respectively. In the repeated-batch fermentation, the yeasts could be used at least eight successive batches without a marked decrease in ethanol production. The repeated-batch fermentation with fill and drain volume at 75% of the working volume gave higher ethanol production efficiencies than those at 50% of the working volume in terms of total ethanol production rate (g h -1). The average P, Q p and Y p/s of the eight successive batches at 75% fill and drain volume in a 2 L bioreactor at the agitation rate of 100 rev min -1 were 93.30 ± 9.44 g l -1 , 1.21 ± 0.43 g l -1 h -1 and 0.48 ± 0.03, respectively.

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Application of a membrane recycle bioreactor for continuous ethanol production

Cited by 29 publications

References 14 publications

Toluene bioconversion to p‐hydroxybenzoate by fed‐batch cultures of recombinant Pseudomonas putida

Toluene bioconversion to p‐hydroxybenzoate by fed‐batch cultures of recombinant Pseudomonas putida

High gravity and high cell density mitigate some of the fermentation inhibitory effects of softwood hydrolysates

Repeated-batch ethanol fermentation from sweet sorghum juice by free cells of Saccharomyces cerevisiae NP 01

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