Application of a micro-Brillouin scattering technique to characterize bone in the GHz range

Matsukawa, Mami; Ryo, Tsubota; Kawabe, Masahiko; Fukui, Kenji

doi:10.1016/j.ultras.2013.09.016

Cited by 18 publications

(15 citation statements)

References 29 publications

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“…17,[28][29][30][31] There are number of works reporting the biomechanics of the hard tissues. [32][33][34][35][36][37] Bone healing at the implant site was investigated using micro-BLS technique and measured ultrasonic velocities in the newly formed bones and mature bones were in a good agreement with the histological analysis. 32 The higher sound velocities, corresponding to the higher elastic modulus were observed for the mature bones having higher mineral content.…”

Section: Introductionmentioning

confidence: 64%

“…Obtained spectra from the bone samples are in a good agreement with those reported in the literature. [35][36][37]40 Figure 4(a) shows examples of Brillouin spectra of the ovine's bone acquired at compressive loads up to 100 kg. Each spectrum corresponds to a specific compression load.…”

Section: Resultsmentioning

confidence: 99%

“…Traditional Brillouin spectroscopy setups take point measurements and long-time measurements of a weak Brillouin scattering light are required (acquisition time about 100 ms). 35,65 To overcome this problem and perform two-dimensional BLS measurements, several configurations are proposed such as introduction of a coherent phonon technique, virtually imaged phased array based either on stimulated or spontaneous Brillouin scattering. 22,65,66 The recent Brillouin microscopy setup designed by Zhang et al 65 is based on spontaneous Brillouin scattering and enables simultaneous measurement of the hundreds of points in a sample with an acquisition time of <1 ms per point and spatial resolution 3.29-μm per pixel close to diffraction limit.…”

Section: Resultsmentioning

confidence: 99%

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Brillouin spectroscopy and radiography for assessment of viscoelastic and regenerative properties of mammalian bones

et al. 2018

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Biomechanical properties of mammalian bones, such as strength, toughness, and plasticity, are essential for understanding how microscopic-scale mechanical features can link to macroscale bones' strength and fracture resistance. We employ Brillouin light scattering (BLS) microspectroscopy for local assessment of elastic properties of bones under compression and the efficacy of the tissue engineering approach based on heparin-conjugated fibrin (HCF) hydrogels, bone morphogenic proteins, and osteogenic stem cells in the regeneration of the bone tissues. BLS is noninvasive and label-free modality for probing viscoelastic properties of tissues that can give information on structure-function properties of normal and pathological tissues. Results showed that MCS and BPMs are critically important for regeneration of elastic and viscous properties, respectively, HCF gels containing combination of all factors had the best effect with complete defect regeneration at week nine after the implantation of bone grafts and that the bones with fully consolidated fractures have higher values of elastic moduli compared with defective bones.

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Section: Introductionmentioning

confidence: 64%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Brillouin spectroscopy and radiography for assessment of viscoelastic and regenerative properties of mammalian bones

et al. 2018

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“…The same scattering geometry used for the BLS characterization of collagene (thin flat sample over a reflective substrate) was also used for the determination of the mechanical properties of bone matrix, and compared with results obtained by nanoindentation and scanning acoustic microscopy [43]. It was shown that, though BLS requires longer acquisition times with respect to the other techniques, it is unique in allowing an easy measure of the in-plane anisotropy of wave velocities for both logitudinal and transverse phonons.…”

Section: Brillouin Spectroscopy From Biomaterialsmentioning

confidence: 99%

Brillouin scattering of phonons in complex materials

Bottani

Fioretto

2018

Advances in Physics: X

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Initially, the theory of propagation of long-wavelength acoustic phonons and Brillouin scattering of laser light in condensed matter is concisely summarized. Then, the case of two relevant classes of complex materials in which Brillouin scattering can be measured is reviewed. First, in lowdensity, low-dimensional, disordered materials, the crossover between confinement and propagation is discussed on the basis of experimental findings. Moreover, the possibility of measuring the local mechanical properties of these materials at the mesoscale by Brillouin scattering is critically discussed. Second the application of Brillouin scattering to biological materials, a rather hot topic, is presented. The acoustic waves and their phonons Though the traditional ways to measure the mechanical properties of materials and, in particular, the elastic constants, are based on quasi-static deformation processes (e.g. the tensile test to measure the Young modulus), many important methods are acoustic in nature or make use of acoustic phenomena (ultrasound

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“…Micro-Brillouin scattering is another technique that can be used to investigate the elastic properties of materials, based on photoelastic effects. Micro-Brillouin scattering allows the assessment of the ultrasonic wave velocity of the tested material in the GHz range, without contact and nondestructively [38]. Micro-Brillouin scattering has been used to investigate bone wave velocity, in particular within the femoral head [39] or in a bone defect [40], as well as to assess bone anisotropic properties [41], bone structure and alignment [42], the effect of decalcification [43] and the effects of glycation [44].…”

Section: Introductionmentioning

confidence: 99%

Multimodal Evaluation of the Spatiotemporal Variations of Periprosthetic Bone Properties

Fraulob

Cann

Voumard

et al. 2020

Journal of Biomechanical Engineering

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Titanium implants are widely used clinically. However, surgical failures still occur because of a lack of implant stability. Periprosthetic biomechanical bone properties need to be characterized to better understand osseointegration phenomena and anticipate implant failures. The aim of this study was toinvestigate the spatio-temporal variations of i) the indentation modulus E* and ii) the wave velocity v of newly formed bone tissue. Eight coin-shaped Ti6Al4V implants were inserted into rabbit tibiae for 7 and 13 weeks. This in vivo model allowed to distinguish mature and newly formed bone and to work in a standardized configuration. Nanoindentation, histological analysis and micro-Brillouin scattering measurements were carried out to assess the indentation modulus E*, the BA/TA (bone area/total area) and the wave velocity v, respectively, at different locations relatively to the implant surface. This approach allowed to determine the relative variation of bone mass density at the microscopic scale. All measured parameters (E*, v, ? and BA/TA) are found to be higher in the vicinity of the implant and for higher healing duration, suggesting increased mineralization. The indentation modulus is 12.3 % (respectively 3.2 %) higher in the close than in the far groups and 14.4 % (respectively 2.8 %) higher in the lateral than in the central groups after 7 weeks (respectively 13 weeks) of healing, leading to similar evolution of the bone mass density. These results lead to a bone spreading pathway confirmed by histology consistent with contact osteogenesis phenomena.

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Application of a micro-Brillouin scattering technique to characterize bone in the GHz range

Cited by 18 publications

References 29 publications

Brillouin spectroscopy and radiography for assessment of viscoelastic and regenerative properties of mammalian bones

Brillouin spectroscopy and radiography for assessment of viscoelastic and regenerative properties of mammalian bones

Brillouin scattering of phonons in complex materials

Multimodal Evaluation of the Spatiotemporal Variations of Periprosthetic Bone Properties

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