Application of a microcomputer-controlled film balance system to collection and analysis of data from mixed monolayers

Brockman, Howard L.; Jones, C.M.; Schwebke, Curt J.; Smaby, Janice M.; Jarvis, Dale E.

doi:10.1016/0021-9797(80)90588-3

Cited by 58 publications

(51 citation statements)

References 8 publications

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“…When second compression of the film was studied, the lipid monolayer was compressed up to 35 mN/m, relaxed by decompression to 0.7 mN/m (in about 5 min), allowed to stand at that pressure for 5 min and subsequently compressed to collapse. The collapse and other phase transition points were estimated by the third derivate method (Brockman et al, 1980). Compressibility modulus (C s −1 ) was calculated from the isotherm data as: C s −1 = −A(d /dA) T (Ali et al, 1991;Mohwald, 1995).…”

Section: Monolayer Isotherms and Isobarsmentioning

confidence: 99%

Phase state and surface topography of palmitoyl-ceramide monolayers

Fanani¹,

Maggio²

2010

Chemistry and Physics of Lipids

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Section: Monolayer Isotherms and Isobarsmentioning

confidence: 99%

Phase state and surface topography of palmitoyl-ceramide monolayers

Fanani¹,

Maggio²

2010

Chemistry and Physics of Lipids

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“…The trough was separately enclosed under humidified argon, cleaned with a seven-stage series filtration set-up consisting of an Alltech activated charcoal gas purifier, a LabClean filter, and a series of Balston disposable filters consisting of two adsorption (carbon) and three filter units (93% and 99.99% efficiency at 0.1 m). Other technical features that contribute to isotherm reproducibility include automated lipid spreading via a modified HPLC autoinjector, automated surface cleaning by multiple barrier sweeps between runs, and highly accurate and reproducible setting of the subphase level by an automated aspirator (Brockman et al, 1980(Brockman et al, , 1984.…”

Section: Monolayer Conditionsmentioning

confidence: 99%

Sphingomyelin Interfacial Behavior: The Impact of Changing Acyl Chain Composition

Smaby

Momsen

et al. 2000

Biophysical Journal

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115

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Sphingomyelins (SMs) containing homogeneous acyl chains with 12, 14, 16, 18, 24, or 26 carbons were synthesized and characterized using an automated Langmuir-type film balance. Surface pressure was monitored as a function of lipid molecular area at constant temperatures between 10 degrees C and 30 degrees C. SM containing lauroyl (12:0) acyl chains displayed only liquid-expanded behavior. Increasing the length of the saturated acyl chain (e.g., 14:0, 16:0, or 18:0) resulted in liquid-expanded to condensed two-dimensional phase transitions at many temperatures in the 10-30 degrees C range. Similar behavior was observed for SMs with lignoceroyl (24:0) or (cerotoyl) 26:0 acyl chains, but isotherms showed only condensed behavior at 10 and 15 degrees C. Insights into the physico-mechanical in-plane interactions occurring within the different SM phases and accompanying changes in SM phase state were provided by analyzing the interfacial area compressibility moduli. At similar surface pressures, SM fluid phases were less compressible than those of phosphatidylcholines with similar chain structures. The area per molecule and compressibility of SM condensed phases depended upon the length of the saturated acyl chain and upon spreading temperature. Spreading of SMs with very long saturated acyl chains at temperatures 30-35 degrees below T(m) resulted in condensed films with lower in-plane compressibilities, but consistently larger cross-sectional molecular areas than the condensed phases achieved by spreading at temperatures only 10-20 degrees below T(m). This behavior is discussed in terms of the enhancement of SM lateral aggregation by temperature reduction, a common approach used during domain isolation from biomembranes.

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“…The temperature was maintained at 24 ± 0.5°C with an external circulating water bath (Haake F3C). The collapse pressure, surface pressure point for molecular reorganization and limiting mean molecular area of the Insulin films were determined from the third derivative of the compression isotherms [8], after being reproduced in at least three independent experiments.…”

Section: Compression Isothermsmentioning

confidence: 99%