Application of a monoclonal antibody specific for the ORF92 capsid protein of Cyprinid herpesvirus 2

Shen, Zhaoyuan; Jiang, Yousheng; Lu, Jian-Fei; Sano, Motohiko; Xu, Dan; Lu, Liqun

doi:10.1016/j.jviromet.2018.07.012

Cited by 13 publications

(9 citation statements)

References 18 publications

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“…Monoclonal antibody mAb‐1B7 was produced against pORF92 and used for the detection of CyHV‐2‐infected crucian carp by western blotting and indirect immunofluorescence assays (IFA) methods (Shen et al . 2018).…”

Section: Diagnosis Of Cyhv‐2 Infectionmentioning

confidence: 99%

“…In another study Kong et al (2017) prepared monoclonal antibodies against recombinant ORF72 protein (pORF72) of CyHV-2 and then established an immunohistochemical protocol and a blood smear method to detect CyHV-2 in carps using the same monoclonal antibody. Monoclonal antibody mAb-1B7 was produced against pORF92 and used for the detection of CyHV-2-infected crucian carp by western blotting and indirect immunofluorescence assays (IFA) methods (Shen et al 2018).…”

Section: Immunological Techniquesmentioning

confidence: 99%

See 1 more Smart Citation

Cyprinid herpesvirus‐2 (CyHV‐2): a comprehensive review

Thangaraj

Nithianantham

Dharmaratnam

et al. 2020

Reviews in Aquaculture

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Cyprinid herpesvirus-2 (CyHV-2) is a linear double-stranded DNA virus in the genus Cyprinivirus of family Alloherpesviridae. The virus is known to be highly pathogenic to ornamental goldfish (Carassius auratus), crucian carp (C. carassius) and Gibel carp (C. auratus gibelio), and also to the hybrids of goldfish and other carps. Cyprinid herpesvirus-2, having the smallest genome (290.3 kb) among Cyprinivirus, causes herpesviral hematopoietic necrosis disease (HVHND) that results in huge economic losses in aquaculture industry as the disease can cause high mortality (50-100%) among the affected fish. The disease was initially reported as the cause of epizootics in juvenile goldfish of Japan during 1992 and 1993. To date, this disease has been reported around the world including Europe, North America, Oceania and Asia. Huge economic losses due to the CyHV-2 infection among cultured gibel carp in China, during 2011-2012, mass mortality in crucian carp during 2012 in Italy, 95% mortality in goldfish during 2014 in France, 85% mortality in goldfish during 2016 in Poland had been reported. Strategies for controlling the spread of CyHV-2 are thus urgently required to limit economic damage. Furthermore, the review will shed light on lacunae in current knowledge as well as on the perspectives that merits further investigations on CyHV-2 research. The paper forms the first comprehensive overview of CyHV-2 causing a serious economically significant fish disease and, will be helpful for the researchers to get all related information from a single manuscript.

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Section: Diagnosis Of Cyhv‐2 Infectionmentioning

confidence: 99%

Section: Immunological Techniquesmentioning

confidence: 99%

Cyprinid herpesvirus‐2 (CyHV‐2): a comprehensive review

Thangaraj

Nithianantham

Dharmaratnam

et al. 2020

Reviews in Aquaculture

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“…The present study was focused to develop RPA primers and probes targeting the highly conserved major capsid protein (MCP) gene (ORF 92) of CyHV-2 for rapid diagnosis of HVHND. Major capsid protein is one of the most widely used biomarkers of viral infection in fish and has often been used as a capture antigen in immunological studies and for vaccine development (Shen et al 2018). To date, there are very few studies on the major capsid protein of CyHV-2, and therefore, this study is the first of its kind focusing the development of an easy and reliable RPA-LFD assay prototype for the POC detection of CyHV-2.…”

Section: Introductionmentioning

confidence: 99%

Quick hassle-free detection of cyprinid herpesvirus 2 (CyHV-2) in goldfish using recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) assay

et al. 2022

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Cyprinid herpesvirus 2 (CyHV-2) is the etiological agent of herpesviral hematopoietic necrosis disease (HVHND), which causes severe mortality in ornamental goldfish (Carassius auratus), crucian carp (Carassius auratus), and gibel/prussian carp (Carassius gibelio). Quick and hassle-free point-of-care detection of CyHV-2 is vital for the maintenance of ornamental fish health. In this manuscript, we describe the development of a rapid and sensitive RPA (recombinase polymerase amplification) assay, coupled with lateral flow dipsticks (LFD), that can achieve sensitive diagnosis of CyHV-2 in goldfish within 20 min at 36 °C with the satisfactory detection limit of 10 2 gene copies per reaction. This is the first report wherein major capsid protein (MCP) of CyHV-2 was targeted for RPA-LFD assay development. The assay did not show any cross-reactivity with other viral pathogens like cyprinid herpesvirus 3 (CyHV-3), spring viremia of carp virus (SVCV), infectious spleen and kidney necrosis virus (ISKNV), and viral nervous necrosis virus (VNNV). Furthermore, screening of CyHV-2 infection in CyHV-2-infected goldfish did not yield any false positive/negative results. In short, the RPA-LFD assay developed in this study presents a simple, rapid, and sensitive method for point-of-care diagnosis of CyHV-2, especially under resource-limited conditions.

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“…However, just a few of CyHV‐2 proteins were identified and characterized, with the help of monoclonal antibodies (mAbs) and polyclonal antibodies. These include the capsid protein pORF72 and pORF92 (Kong et al., 2017; Shen et al., 2018), the integral membrane proteins pORF25 (Wu et al., 2020; Zhou et al., 2015) and ORF104 (Du et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

Generation and application of a monoclonal antibody specific for the ORF121 of cyprinid herpesvirus 2

Gao

Zhao

Zheng

et al. 2021

Journal of Fish Diseases

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Cyprinid herpesvirus 2 (CyHV‐2) is a viral pathogen worldwide and causing high mortality on goldfish and silver crucian carp (Carassius auratus gibelio). In order to establish a stable and sensitive immunological diagnostic approach, the recombinant ORF121 protein encoded by the CyHV‐2 ORF121 gene, was selected as a capture antigen to identify cells and tissues infected with CyHV‐2 by immunological methods in this study. Firstly, the open reading frame of CyHV‐2 ORF121 was cloned into the PGEX‐4T‐3 vector and expressed in Escherichia coli. Purified recombinant ORF121 protein was then used as an antigen to prepare monoclonal antibodies, and an efficient hybridoma cell line was selected by dot‐blot assay. The resulting mAb‐3D9 was applied to detect CyHV‐2 in infected caudal fin of Carassius auratus gibelio (GiCF) cells and fish tissues by western blotting, immunofluorescence assays and immunohistological asays. The monoclonal antibody could specifically identify CyHV‐2 in infected GiCF cells and the gills, the kidney and the spleen tissues, and it could attenuate CPE by CyHV‐2 in vitro, suggesting it can be applied for CyHV‐2 detection in the crucian carp and ORF121 may be a candidate vaccine against CyHV‐2.

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Application of a monoclonal antibody specific for the ORF92 capsid protein of Cyprinid herpesvirus 2

Cited by 13 publications

References 18 publications

Cyprinid herpesvirus‐2 (CyHV‐2): a comprehensive review

Cyprinid herpesvirus‐2 (CyHV‐2): a comprehensive review

Quick hassle-free detection of cyprinid herpesvirus 2 (CyHV-2) in goldfish using recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) assay

Generation and application of a monoclonal antibody specific for the ORF121 of cyprinid herpesvirus 2

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