Application of a multiphase microreactor chemostat for the determination of reaction kinetics of Staphylococcus carnosus

Abstract

Bioreactors at the microliter-scale offer a promising approach to accelerate bioprocess development. Advantages of such microbioreactors include a reduction in the use of expensive reagents. In this study, a chemostat operation mode of a cuvette-based microbubble column-bioreactor made of polystyrene (working volume of 550 μL) was demonstrated. Aeration occurs through a nozzle (Ø ≤ 100 μm) and supports submerged whole-cell cultivation of Staphylococcus carnosus. Stationary concentrations of biomass and glucose were determined in the dilution rate regime ranging from 0.12 to 0.80 1/h with a glucose feed concentration of 1 g/L. For the first time reaction kinetics of S. carnosus were estimated from data obtained from continuous cultivation. The maximal specific growth rate (µ max = 0.824 1/h), Monod constant (K S = 34•10 3 g S /L), substrate-related biomass yield coefficient (Y X/S = 0.315 g CDW /g S), and maintenance coefficient (m S = 0.0035 g S /(g CDW •h)) were determined. These parameters are now available for further studies in the field of synthetic biology.

Fatty Acid Methyl Ester Synthesis through Transesterification of Palm Oil with Methanol in Microchannels: Flow Pattern and Reaction Kinetics

“…Reaction kinetics was always measured in a batch reactor equipped with a stirrer. With the rapid development of microchemical technology, microreactors have shown the potential to replace the traditional batch reactors to become a novel measuring tool for reaction kinetics. − In comparison with batch reactors, microreactors have the advantages of reducing mass-transfer resistance to acquire intrinsic kinetics and of collecting kinetics data at the first seconds of the reaction. A few research studies tested their performance in determining the reaction kinetics of FAME synthesis. − Santacesaria et al , investigated the reaction kinetics for ethanolysis of soybean oil in a packing bed microreactor with a hydrodynamic diameter in the range of 300–1000 μm, finding that the reaction would not be limited by mass transfer.…”

Reaction kinetics of anodic biofilms under changing substrate concentrations: Uncovering shifts in Nernst‐Monod curves via substrate pulses

“…Lee et al worked at -0.4 V versus Ag/AgCl whereas Zarabadi et al did not specify the applied anode potential during biofilm growth [27,28]. In literature, Monod constant values of non-electroactive planktonically cultivated Escherichia coli and Saccharomyces cerevisiae vary over more than 2 and 3 orders of magnitude, and it should be stressed that the case of E. coli and S. cerevisiae does not stand alone [29]. K S depends on the respective substrate, the concentration range and the chemostat conditions.…”

3D-printed micro bubble column reactor with integrated microsensors for biotechnological applications: From design to evaluation

“…A cuvette MBR with online sensor technology for pH, OD, DOT, and glucose measurements was also recently developed 11 . The functionality and the high potential of the cuvette MBR has been demonstrated for cultivation in batch mode with S. cerevisiae 11 and chemostat mode with Staphyllococcus carnosus 12 . Importantly, however, all of these systems are prototypes developed specifically for use in their respective applications.…”