Application of a New Iron Reagent, 3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine, to Spectrophotometric Determination of Tocopherols

Kanno, Choemon; Yamauchi, Kunio

doi:10.1080/00021369.1977.10862541

Cited by 2 publications

(2 citation statements)

References 2 publications

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“…After the centrifugation, the top layer was removed, and the hexane extraction was repeated twice. The assay mixture was prepared as per Kanno and Yamauchi's (1977) method. A total of 0.5 mL of the hexane-extract was added into 0.4 mL 0.1% (w/v) PDT (3-(2-pyridyl)-5,6-diphenyl-1,2,4 triazine, prepared in ethanol) and 0.4 mL 0.1% (w/v) ferric chloride (prepared in ethanol).…”

Section: Non-enzymatic Antioxidant Assaysmentioning

confidence: 99%

Antioxidative responses of Melon Manis Terengganu (MMT) against Fusarium sp.

Baharudin, N. M.,

Rani, N. F. W. A.,

Ibrahim, N. F.

et al. 2024

MJM

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Aims: Melon Manis Terengganu, MMT is one of the economically important fruits in Terengganu, which contains numerous nutritional values and bioactive compounds that benefit human health. The major problem is MMT has been affected by Fusarium sp., which is the common fungus in the Cucurbitaceae family resulting in Fusarium wilt disease and lowering melon production. It may also affect the antioxidant value of MMT; however, limited study has been conducted on this issue. Hence, the objective of this study was to determine the non-enzymatic as well as enzymatic activities in response to Fusarium sp. (S2 and S4) infection. Methodology and results: In this study, MMT leaves were incubated in culture filtrate (CF) obtained from Murashige and Skoog (MS) liquid medium. The antioxidative responses were assayed at 0, 1, 3, 5, 7 and 9 days of treatment. In response to Fusarium infection, the ascorbic acid, α-tocopherol and carotenoid content were significantly stimulated at the early stages of the experiment and slowly reduced afterward. This current study also demonstrated that the CATspecific activities were initially induced in S2 CF-treated leaves. Similar APX and gPOD specific activity patterns were observed in both S2 and S4 CFs treatments. The APX and gPOD-specific activities were induced at the later stages of infection in S4 CF-treated leaves. Conclusion, significance and impact of study:The results revealed that enzymatic and non-enzymatic antioxidants worked together to fight against stress caused by the fungal infection, with the activation of the plant defense system.

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Section: Non-enzymatic Antioxidant Assaysmentioning

confidence: 99%

Antioxidative responses of Melon Manis Terengganu (MMT) against Fusarium sp.

Baharudin, N. M.,

Rani, N. F. W. A.,

Ibrahim, N. F.

et al. 2024

MJM

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show abstract

“…After the centrifugation, the top layer was removed and the hexane extraction was repeated twice. The assay mixture was prepared as described by Kanno and Yamauchi (1977). A total of 0.5 mL of the hexane-extract was added into 0.4 mL 0.1% (w/v) PDT (3-(2-pyridyl)-5,6-diphenyl-1,2,4 triazine) and 0.4 mL 0.1% (w/v) ferric chloride.…”

Section: Non-enzymatic Antioxidant Assaysmentioning

confidence: 99%

Effects of Colletotrichum capsici infection on the growth and antioxidative response on defense mechanisms of Capsicum annuum

Yusuf, N.,

Mohd Fazi, S.,

Ali, N. A.

et al. 2016

MJM

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Aims: Capsicum annuum (pepper) is one of the most important crops in the world. It contains an impressive list of phytonutrients that were found to have disease-preventing and health-promoting properties. Today, they are grown widely in many parts of the world as an important commercial crop. However, fungal infection is the main problem that leads to the common pepper disruptive disease, known as the Anthracnose, which lowers the yields of this plant. Therefore, this study was conducted to determine the growth, enzymatic antioxidant specific activities of catalase (CAT), ascorbate peroxidase (APX), and guaiacol peroxidase (g-POD) as well as non-enzymatic antioxidants including ascorbic acid, α-tocopherol and carotenoids content in C. annuum as a response to the fungal infections by Colletotrichum capsici. Methodology and results:The assays were conducted at 0, 1, 2, 3, 4 and 5 weeks of fungal treatment periods. The results showed that plant growth (plant height, leaves number, length and width, chlorophyll content) was significantly lower in treated plants compared to controls. The CAT specific activity increased drastically at the early stages of the experiment and decreased thereafter. In contrast, the APX and g-POD specific activity were initially lower, but increased significantly at the later stages of the experiment. For the non-enzymatic antioxidants, the amount of ascorbic acid, αtocopherol and carotenoids content were significantly higher at the first week of treatment and slowly reduced afterwards. Conclusion, significance and impact of study:This study shows that fungal infection inhibited the growth of pepper plants and the enzymatic and non-enzymatic antioxidants worked in concert to fight against the stress caused by the fungal infection, with their different specific roles in removing and reducing the reactive oxygen species in stress condition.

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Application of a New Iron Reagent, 3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine, to Spectrophotometric Determination of Tocopherols

Cited by 2 publications

References 2 publications

Antioxidative responses of Melon Manis Terengganu (MMT) against Fusarium sp.

Antioxidative responses of Melon Manis Terengganu (MMT) against Fusarium sp.

Effects of Colletotrichum capsici infection on the growth and antioxidative response on defense mechanisms of Capsicum annuum

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