Application of a new oxidation-reduction potential assessment method in strenuous exercise-induced oxidative stress

Stagos, Dimitrios; Goutzourelas, Nikolaos; Bar‐Or, David; Ntontou, Amalia-Maria; Bella, Evangelia; Becker, Aphrodite Tousia; Statiri, A; Kafantaris, Ioannis; Kouretas, Demetrios

doi:10.1179/1351000214y.0000000118

Cited by 43 publications

(47 citation statements)

References 45 publications

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“…Recently, a novel technology based on a galvanostatic measure of electrons has been developed that easily and readily measures ORP -the MiOXSYS System (Figure 2). This system has been used to assess changes in oxidative stress in trauma patients and as a function of extreme exercise [Rael et al 2007[Rael et al , 2009Stagos et al 2015aStagos et al , 2015b. Data from the MiOXSYS System are presented in static ORP measures (sORP), with static referring to the passive or current state of activity between oxidants and antioxidants.…”

Section: Oxidation-reduction Potential: Measurement and Clinical Utilitymentioning

confidence: 99%

Oxidation-reduction potential of semen: what is its role in the treatment of male infertility?

Agarwal

Roychoudhury

Bjugstad³

et al. 2016

Therapeutic Advances in Urology

140

118

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The diagnosis of male infertility relies largely on conventional semen analysis, and its interpretation has a profound influence on subsequent management of patients. Despite poor correlation between conventional semen parameters and male fertility potential, inclusion of advanced semen quality tests to routine male infertility workup algorithms has not been widely accepted. Oxidative stress is one of the major mediators in various etiologies of male infertility; it has deleterious effects on spermatozoa, including DNA damage. Alleviation of oxidative stress constitutes a potential treatment strategy for male infertility. Measurement of seminal oxidative stress is of crucial role in the identification and monitoring of patients who may benefit from treatments. Various tests including reactive oxygen species (ROS) assay, total antioxidant capacity (TAC) assay or malondialdehyde (MDA) assay used by different laboratories have their own drawbacks. Oxidation-reduction potential (ORP) is a measure of overall balance between oxidants and antioxidants, providing a comprehensive measure of oxidative stress. The MiOXSYS™ System is a novel technology based on a galvanostatic measure of electrons; it presents static ORP (sORP) measures with static referring to the passive or current state of activity between oxidants and antioxidants. Preliminary studies have correlated sORP to poor semen qualities. It is potentially useful in prognostication of assisted reproductive techniques outcomes, screening of antioxidants either in vivo or during IVF cycles, identification of infertile men who may benefit from treatment of oxidative stress, and monitoring of treatment success. The simplified laboratory test requiring a small amount of semen would facilitate clinical application and research in the field. In this paper, we discuss the measurement of ORP by the MiOXSYS System as a real-time assessment of seminal oxidative stress, and argue that it is a potential valuable clinical test that should be incorporated into the male infertility workup and become an important guide to the treatment of oxidative stress-induced male infertility.

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Section: Oxidation-reduction Potential: Measurement and Clinical Utilitymentioning

confidence: 99%

Oxidation-reduction potential of semen: what is its role in the treatment of male infertility?

Agarwal

Roychoudhury

Bjugstad³

et al. 2016

Therapeutic Advances in Urology

140

118

View full text Add to dashboard Cite

show abstract

“…These two novel markers have been used previously in several of our studies for assessing exercise-induced oxidative stress [7][8][9]45]. The above correlations were meaningful, since sORP increased values correspond to higher oxidative stress levels, as it represents the integrated balance of oxidants and reductants, while cORP is a measure of antioxidant reserve available in the body's system.…”

Section: Discussionmentioning

confidence: 80%

“…For the determination of oxidative stress levels after exercise, a number of oxidative stress markers are assessed such as the levels and activity of antioxidant enzymes and molecules, oxidative DNA damage, lipid peroxidation and protein oxidation [7][8][9] Regarding protein oxidation, it is usually assessed by measuring protein carbonyl levels (PC) in plasma. The most abundant protein in plasma (about 50% of total protein) is human serum albumin (HSA) [10].…”

Section: Introductionmentioning

confidence: 99%

Oxidation of Human Serum Albumin Exhibits Inter-Individual Variability after an Ultra-Marathon Mountain Race

Spanidis

Priftis

Stagos

et al. 2017

Preprint

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Abstract:The aim of this study was to examine the oxidation of human serum albumin (HSA) caused by oxidative stress after an exhaustive exercise such as ultramarathon race. Thus, blood samples from 12 adult runners who underwent a 103 km mountain ultra-marathon race were collected pre-and 24, 48 and 72 h post race. HSA was partially purified using affinity chromatography and then was subjected to Western blot analysis for disulfide dimers determination, indicating oxidation. The results were correlated with those from a previous study in which the same samples were analyzed using different oxidative stress markers and a good correlation with protein carbonyls (PC) at all time points was observed. Moreover there was a significant correlation with static oxidation-reduction potential (sORP) at 24 h, and a negative correlation with capacity oxidation-reduction potential (cORP) at 24 and 48 h. In addition, an individual analysis of albumin dimers exhibited great interindividual differences. This inter-individual variability in the oxidation of HSA may suggest different interventions (e.g. through diet) in order to confront the effects on athletes' organism after a strenuous exercise. In conclusion, this study supported the importance of the assessment of albumin dimers as a predictive marker for exerciseinduced oxidative stress.

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“…Oxidation‐Reduction Potential (ORP) was measured with 30 μL serum using the Redox SYS Analyzer (Bio Research Center, Nagoya, Japan). The degree of oxidation (sORP [mV]) was defined as the increase in ORP, and the degree of antioxidation was defined as the decrease in cORP (μC) …”

Section: Oxidative Stress Measurementmentioning

confidence: 99%

“…The degree of oxidation (sORP [mV]) was defined as the increase in ORP, and the degree of antioxidation was defined as the decrease in cORP (lC). 7…”

Section: Oxidative Stress Measurementmentioning

confidence: 99%

The effect of small dose of topiroxostat on serum uric acid in patients receiving hemodialysis

Nagaoka

Tanaka

Yoshimoto

et al. 2017

Hemodialysis International

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Application of a new oxidation-reduction potential assessment method in strenuous exercise-induced oxidative stress

Abstract: The present study demonstrated for the first time that the RedoxSYS diagnostic system can be used for evaluating the exercise-induced oxidative stress in athletes.

Cited by 43 publications

References 45 publications

Oxidation-reduction potential of semen: what is its role in the treatment of male infertility?

Oxidation-reduction potential of semen: what is its role in the treatment of male infertility?

Oxidation of Human Serum Albumin Exhibits Inter-Individual Variability after an Ultra-Marathon Mountain Race

The effect of small dose of topiroxostat on serum uric acid in patients receiving hemodialysis

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