Application of a quantitative 1H-NMR method for the determination of paeonol in Moutan cortex, Hachimijiogan and Keishibukuryogan

Abstract

Quantitative (1)H-NMR ((1)H-qNMR) was applied to the determination of paeonol concentration in Moutan cortex, Hachimijiogan, and Keishibukuryogan. Paeonol is a major component of Moutan cortex, and its purity was calculated from the ratio of the intensity of the paeonol H-3' signal at δ 6.41 ppm in methanol-d 4 or 6.40 ppm in methanol-d 4 + TFA-d to that of a hexamethyldisilane (HMD) signal at 0 ppm. The concentration of HMD was corrected with SI traceability by using potassium hydrogen phthalate of certified reference material grade. As a result, the paeonol content in two lots of Moutan cortex as determined by (1)H-qNMR was found to be 1.59 % and 1.62 %, respectively, while the paeonol content in Hachimijiogan and Keishibukuryogan was 0.15 % and 0.22 %, respectively. The present study demonstrated that the (1)H-NMR method is useful for the quantitative analysis of crude drugs and Kampo formulas.

Chemical Fingerprinting and Quantitative Analysis of Cortex Moutan From Different Tree Peony Cultivars Using HPLC-ESI/MS

“…8,10 Pharmacological evidence suggests that paeonol has antibacterial, antiaggregation, antianxiety, and antiinflammation effects. 8,[10][11][12][13][14] In fact, unlike single chemical entities aimed at a specific single target, traditional Chinese medicines are complex mixtures containing hundreds of diverse chemical constituents. 15 Therefore, traditional Chinese medicines are too complicated to investigate comprehensively their quality consistency effectively with a single detection technique.…”

Determination of PAHs in Solution with a Single Reference Standard by a Combination of 1H Quantitative NMR Spectroscopy and Chromatography

“…Recently 1 H NMR has been dramatically improved as a quantitative technique, leading to its widespread use in a technique called 1 H quantitative NMR spectroscopy ( 1 H-qNMR). − One of the most important benefits of 1 H-qNMR is nonspecificity. Various organic compounds can be accurately determined by 1 H-qNMR based on the number of protons in molecules using only one reference standard.…”

Origins, Phytochemistry, Pharmacology, Analytical Methods and Safety of Cortex Moutan (Paeonia suffruticosa Andrew): A Systematic Review

“…Recently, quantitative 1 H-NMR ( 1 H-qNMR) was applied to the determination of paeonol ( 83 ) concentration in CM, Hachimijiogan, and Keishibukuryogan [ 149 ]. The 1 H-qNMR method has many advantages, it requires neither reference compounds for establishing calibration curves nor sample pre-purification, but it is limited by its inherent low sensitivity.…”