Application of a rapid glucose-6-phosphate isomerase assay in white cabbage breeding

Abstract

In the studied conditions of cellulose acetate electrophoresis only glucose-6-phosphate isomerase (PGI, EC 5.3.1.9) appeared to be a polymorphic isozyme. The PGI analysis of 23 cabbage (Brassica oleracea var. capitata) inbred lines, carried out in two years, allowed detecting off-type individuals in four lines. Seed contamination caused by sib-pollination was detected in 10 out of 19 Ft hybrids. In five of them the contamination did not exceed 5 %, and in the remaining five, ranged from 27-52 %. Also homozygosity of 67 plants obtained through anther culture in vitro was confirmed. The results indicate that PGI electrophoresis in cellulose acetate matrix is very fast and can be useful in the assessment of genetic purity in cabbage breeding materials.

In vitro culture of unfertilized ovules in carrot (Daucus carota L.)

“…After 3 months, the roots were planted in pots containing a mixture of peat moss substrate, high peat moss, and coarse sand (2:1:1 v/v), and grown in the greenhouse. At the phase of 4-6 well-developed leaves, carrot plants were selected on the basis of their phosphoglucoisomerase (PGI) isozyme profile (Barański 2000). Individuals heterozygous for the Pgi-2 locus were used as ovule donors.…”

“…Electrophoresis was performed at room temperature using 0.025 M Tris-glycine pH 8.0 buffer. Staining was done as described by Barański (2000).…”

Induction conditions for somatic and microspore-derived structures and detection of haploid status by isozyme analysis in anther culture of caraway (Carum carvi L.)

“…In caraway, both forms of EST, monomer and dimer, or their combination were present. A heterozygous profile of donor plants is a prerequisite for comparing donor plants and their antherderived generations (Baranski 2000). If the donor plants were identified as heterozygous at any EST locus, the true DH plants were determined.…”

“…The second possibility is having a method which uses molecular markers for identification of the origin of the embryos (Schmidt et al 1997;Rodriques et al 2005). However, for practical reasons, it is best to have a quick and cost-effective method, such as isozyme analysis (Baranski 2000;Bartošová et al 2005), which can characterize androgenetic doubled haploids. For efficient identification using these methods, such as a good marker system, it is necessary to have a heterozygous profile in the donor/parental plants (control).…”

“…For efficient identification using these methods, such as a good marker system, it is necessary to have a heterozygous profile in the donor/parental plants (control). Adamus and Michalik (2003) utilized the PGI isozyme system (Baranski 2000) to choose their donor plant material and later to distinguish the origin of the DH-regenerated plants. This biochemical technique detected genetic variation in caraway, in which a variety is created as a heterogamous population.…”