Application of a stability-indicating HPTLC method for quantitative analysis of sertraline hydrochloride in pharmaceutical dosage forms

New TLC Method Combined with Densitometry for Determination of Sertraline and Fluoxetine in Pharmaceutical Preparations

“…The obtained results can be compared to the HPTLC stability studies of sertraline carried out in previous years. Namely, Rao J. et al [15] used HPTLC plates precoated with silica gel 60F 254 as the stationary phase, while the mobile phase consisted of toluene, ethyl acetate, ethanol, and ammonia in a volume ratio of 8: 2: 0.5: 0.1. In this study, to test the chemical stability of sertraline, TLC plates precoated with silica gel 60F 254 were also used, and the mobile phase used in the study by Rao J. et al [15], which was marked with the letter I; however, this did not produce favorable results, because there was no separation of degradation products from sertraline (degradation substances remained immediately above the starting point).…”

“…Namely, Rao J. et al [15] used HPTLC plates precoated with silica gel 60F 254 as the stationary phase, while the mobile phase consisted of toluene, ethyl acetate, ethanol, and ammonia in a volume ratio of 8: 2: 0.5: 0.1. In this study, to test the chemical stability of sertraline, TLC plates precoated with silica gel 60F 254 were also used, and the mobile phase used in the study by Rao J. et al [15], which was marked with the letter I; however, this did not produce favorable results, because there was no separation of degradation products from sertraline (degradation substances remained immediately above the starting point). Rao J. et al [15] showed that sertraline was degraded in an acidic environment (one degradation product was formed), in an alkaline environment (two degradation products were formed), after oxidation (two degradation products were formed), and after water hydrolysis (one degradation product was formed).…”

“…In this study, to test the chemical stability of sertraline, TLC plates precoated with silica gel 60F 254 were also used, and the mobile phase used in the study by Rao J. et al [15], which was marked with the letter I; however, this did not produce favorable results, because there was no separation of degradation products from sertraline (degradation substances remained immediately above the starting point). Rao J. et al [15] showed that sertraline was degraded in an acidic environment (one degradation product was formed), in an alkaline environment (two degradation products were formed), after oxidation (two degradation products were formed), and after water hydrolysis (one degradation product was formed). The separation of sertraline from its degradation products using the M mobile phase in the presented work took place under similar stress conditions.…”

“…There are reports in scientific journals about the use of various analytical methods for the determination of antidepressants, including fluoxetine and sertraline. The tests were carried out on various types of samples, such as pharmaceutical preparations, whole blood, plasma, and others, using, among others, thin layer chromatography (TLC) or high performance thin layer chromatography (HPTLC) [12][13][14][15][16][17][18][19][20][21][22], high performance liquid chromatography (HPLC) [16,17,[23][24][25][26][27][28][29][30][31][32][33], ultra-performance liquid chromatography (UPLC) [34][35][36][37][38][39][40], and gas chromatography-mass spectrometry (GC/MS) [41][42][43][44][45][46][47], in addition to spectrophotometry [48][49][50], spectrofluorimetry [51], capillary electrophoresis [52,53], differential impulse voltammetry…”

Determination of some psychotropic drugs in human plasma by HPTLC

“…Thin layer chromatography is one of the most important methods in the field of pharmaceutical analysis [1][2][3]. TLC is used from the first test in the synthetic research laboratory, to follow the synthesis of a new entity, to the quality control of the finished products.…”