Application of a strategy based on metabolomics guided promoting blood circulation bioactivity compounds screening of vinegar

Ning, Zhangchi; Liu, Zhenli; Song, Zhilong; Chun, Wu; Liu, Yuanyan; Jiahe, Gan; Ma, Xu; Lü, Aiping

doi:10.1186/s13065-017-0265-5

Cited by 5 publications

(3 citation statements)

References 28 publications

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“…Among the four in vitro coagulation assays of prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT) and (fibrinogen) FIB [ 30 ] are the most commonly used screening tests to reflect the exogenous and endogenous coagulation pathways, respectively, while TT is used to detect the contribution of prothrombin to thrombin production and FIB is used as a fibrinogen assay.…”

Section: Resultsmentioning

confidence: 99%

Optimization Preparation and Evaluation of Chitosan Grafted Norfloxacin as a Hemostatic Sponge

et al. 2023

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Considering the great harm to the human body caused by severe and massive bleeding, in this study, chitosan-grafted norfloxacin (CTS-NF) composites were prepared with chitosan (CTS) and norfloxacin (NF) as raw materials by a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide-mediated coupling method to solve the limitations of slow hemostatic and poor anti-infective effects of current dressings on the market. The effects of the mass ratio of CTS to NF (MCTS/MNF), reaction temperature T and reaction time t on the grafting rate (η%) of the products were investigated through single factor tests. The preparation process was optimized with the η% as an evaluation index by means of the Box–Behnken test design and response surface analysis. The antimicrobial activity was evaluated by inhibition zone assay, and the hemostatic activity of the prepared composites was evaluated in vitro and in vivo. The results suggested that the optimum preparation conditions were the mass ratio of CTS to NF( MCTS/MNF) 5:3, reaction temperature 65 °C, and reaction time 4 h. Under this condition, the η% of CTS-NF was 45.5%. The CTS-NF composites displayed significant antimicrobial activities. Moreover, in vitro hemostasis results revealed that the CTS-NF composite had a lower blood clotting index and absorbed red blood cells to promote aggregation. In vivo ear and live hemostasis, the CTS-NF groups showed short hemostatic time (49.75 ± 3.32 s and 50.00 ± 7.21 s) and more blood loss(0.07 ± 0.010 g and 0.075 ± 0.013 g). The results showed that CTS-NF reduced the bleeding time and volume, exhibiting a significant coagulation effect. Therefore, the CTS-NF sponge is expected to be a new, effective hemostatic and antibacterial material in the future.

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Section: Resultsmentioning

confidence: 99%

Optimization Preparation and Evaluation of Chitosan Grafted Norfloxacin as a Hemostatic Sponge

et al. 2023

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“…The processed frankincense was prepared by stir-frying with rice vinegar according to the procedure recorded in the Chinese Pharmacopoeia (2015 edition). Briefly, 200 g frankincense was put into a heated pot at the temperature 240°C, stir-fried quickly for 9 min and sprayed with 20 mL rice vinegar [this component has been investigated (Ning et al, 2017a)] during the next 2 min until the surface became glossy (Ning et al, 2017b). All the raw and processed samples were vacuum-dried and pulverized into a fine powder using an 80-mesh sieve.…”

Section: Methodsmentioning

confidence: 99%

Integrating Strategies of Herbal Metabolomics, Network Pharmacology, and Experiment Validation to Investigate Frankincense Processing Effects

et al. 2018

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In-depth research on processing can promote the globalization of processed herbs. The purpose of this study is to propose an improved strategy for processing effect investigation. Frankincense and processed frankincense were used as research subjects. First, high-speed countercurrent chromatography (HSCCC) and preparation high-performance liquid chromatography (PHPLC) techniques were used for major compounds isolation and minor compounds concentration. Processed frankincense was subjected to two stepwise solvent systems, namely, n-hexane:ethanol:water (6:5:1) and n-hexane:methyl-acetate:acetonitrile:water (4:4:3:4), to yield 12 fractions, and 18 compounds were further separated. Second, a comprehensive metabolomic analysis conducted by ultrahigh-performance liquid-chromatography/electrospray-ionization mass spectrometry (UHPLC-Qtof-MS) coupled with multivariate statistics was performed to fully characterize the chemical components and discover the potential biomarkers between frankincense and processed frankincense. In total, 81 metabolites, including the 18 separated compounds, were selected as potential biomarkers between frankincense and processed frankincense among 153 detected compounds for their VIP values of greater than one. The tirucallane-type compounds and components with 9,11-dehydro structures clearly occurred at high levels in the processed frankincense, while lupine-type compounds and those with 11-keto structures were significantly higher in frankincense. Then, a network pharmacology model was constructed to decipher the potential mechanisms of processing. Intestinal absorption properties prediction indicated the possibility of processing-related absorption enhancement. A systematic analysis of the constructed networks showed that the C-T network was constructed with 18 potential biomarkers and 69 targets. TNF and IL-1β were among the top-ranked and were linked by 8 and 7 pathways, which were mainly involved in inflammation. The arachidonic acid metabolism pathway exhibited the highest number of target connections. Finally, the prediction was validated experimentally by an intestinal permeability and efficacy assay. The experiments provided convincing evidence that processed frankincense harbored stronger inhibition effects toward TNF-α-, IL-1β- and arachidonic acid-induced platelet aggregation. The processing procedure leads to changes of the chemical metabolites, which triggers the enhancement of absorption and cure efficiency. The global change of the metabolites, absorption and pharmacological effects of processing were depicted in a systematic manner.

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“…Vinegar is composed of various organic acids, amino acids, and volatile compounds that originate from raw materials and are produced by microbial fermentation. Different metabolite profiles of vinegar contribute to different flavors and functional properties [16,17]. Previously, non-volatile metabolites of Korean traditional vinegars were compared to those of commercial vinegars, and the presence and levels of some sugars and acids were identified as being significantly different between the two groups [18].…”

Section: Introductionmentioning

confidence: 99%

Comparative Metabolite Profiling of Traditional and Commercial Vinegars in Korea

Shin

Kim

et al. 2021

Metabolites

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Vinegar, composed of various organic acids, amino acids, and volatile compounds, has been newly recognized as a functional food with health benefits. Vinegar is produced through alcoholic fermentation of various raw materials followed by acetic acid fermentation, and detailed processes greatly vary between different vinegar products. This study performed metabolite profiling of various vinegar products using gas chromatography–mass spectrometry to identify metabolites that are specific to vinegar production processes. In particular, seven traditional vinegars that underwent spontaneous and slow alcoholic and acetic acid fermentations were compared to four commercial vinegars that were produced through fast acetic acid fermentation using distilled ethanol. A total of 102 volatile and 78 nonvolatile compounds were detected, and the principal component analysis of metabolites clearly distinguished between the traditional and commercial vinegars. Ten metabolites were identified as specific or significantly different compounds depending on vinegar production processes, most of which had originated from complex microbial metabolism during traditional vinegar fermentation. These process-specific compounds of vinegars may serve as potential biomarkers for fermentation process controls as well as authenticity and quality evaluation.

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Application of a strategy based on metabolomics guided promoting blood circulation bioactivity compounds screening of vinegar

Cited by 5 publications

References 28 publications

Optimization Preparation and Evaluation of Chitosan Grafted Norfloxacin as a Hemostatic Sponge

Optimization Preparation and Evaluation of Chitosan Grafted Norfloxacin as a Hemostatic Sponge

Integrating Strategies of Herbal Metabolomics, Network Pharmacology, and Experiment Validation to Investigate Frankincense Processing Effects

Comparative Metabolite Profiling of Traditional and Commercial Vinegars in Korea

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