Application of a Surface Plasmon Resonance Sensor to Analyses of Amine Compounds with the Use of a Polymer Film and an Acid-base Reaction

Abstract

Preparation of the PAA filmAn aqueous solution of PAA (0.0025 wt%) was prepared. A surface plasmon resonance (SPR) sensor was applied to analyses of some amine compounds (n-butylamine, isobutylamine, aniline, and N,N-dimethylaniline) by using a polymer film and an acid-base reaction in it. Poly(acrylamide) (PAA) was adopted as the polymer film and was immobilized on an Au film to prepare a sensor chip. Pivalic acid was entered into the PAA film as an acid. The PAA film with a thickness of 50 nm gave the highest sensitivity to the SPR sensor. Although water was better concerning the sensitivity for the SPR sensor as the solvent, ethanol was adopted because it dissolves well all of the amine compounds used. The Au film coated with the PAA film gave higher sensitivity for analyses of n-butylamine and isobutylamine, and lower sensitivity for analyses of aniline and N,N-dimethylaniline than an Au film without the PAA film. The PAA film containing pivalic acid gave 4 -5 orders of magnitude higher sensitivity to the SPR sensor for analyses of all the amine compounds due to the reaction between pivalic acid and these amine compounds.

Sensing Escherichia coli O157:H7 via frequency shift through a self-assembled monolayer based QCM immunosensor

“…PEI and PAA are the materials used for deposition of cationic and anionic layers, which are rich in amido groups and carboxyl groups, respectively. The PEI, as a polycation, was used to form the sensing film of a toxic gas-detecting device (Kikuchi and Shiratori, 2005); the PAA, as a polyanion, was applied to shape a polymer film on the surface of surface plasmon resonance (SPR) to analyze amine compounds (Nishimura et al, 2002). In this report, a polymeric structure based on LBL-SA was formed by PEI + and PAA − for improving the biosensor fabrication.…”

Molecular selectivity development of Teflon® AF1600-coated gold-deposited surface plasmon resonance-based glass rod sensor

“…The resonance angle or the wavelength at which the coupling of the incident light and the surface plasmon waves occurs depends on both the dielectric constant of the deposited metal and the refractive index of the sample. Because the SPR sensor system only responds to the refractive index of the sample under test, many researchers have attempted to add selectivities for specific analytes to these systems [1][2][3][6][7][8][9][10][11][12][13][14][15]. Combination of the SPR sensor with a biochemical reaction, e.g., an antigen-antibody reaction or an enzyme reaction, allows the detection of specific molecules [1][2][3]7,12].…”

“…Combination of the SPR sensor with a biochemical reaction, e.g., an antigen-antibody reaction or an enzyme reaction, allows the detection of specific molecules [1][2][3]7,12]. Selective layers for the SPR sensing elements using an acid-base reaction [10], complex formation between an ionophore and a metal ion [11,13], molecularly imprinted polymers [8,9,15], and diffusion in agar [14] were also investigated. However, these biochemical and chemical selective layers have short lifetimes because they degenerate.…”

Probing of a surface plasma wave by an obliquely incident laser on the metal surface

“…Since the properties of SPW depend strongly on the properties of the interface along which they propagate, they have found various potential applications. They are useful in characterization of metallic surfaces and thin films, 6 biochemical sensing, [7][8][9][10] determination of refractive index of a media. 11 SPW also causes enhancement of many physical phenomenon such as second harmonic generation, 12 Raman scattering, 13,14 electron acceleration, 15,16 material ablation, 17 etc.…”