A new spectral two-step preconditioning of multilevel fast multipole algorithm (MLFMA) is proposed to solve large dense linear systems with multiple right-hand sides arising in monostatic radar cross section (RCS) calculations. The first system is solved with a deflated generalized minimal residual (GMRES) method and the eigenvector information is generated at the same time. Based on this eigenvector information, a spectral preconditioner is defined and combined with a previously constructed sparse approximate inverse (SAI) preconditioner in a two-step manner, resulting in the proposed spectral two-step preconditioner. Restarted GMRES with the newly constructed spectral two-step preconditioner is considered as the iterative method for solving subsequent systems and the MLFMA is used to speed up the matrix-vector product operations. Numerical experiments indicate that the new preconditioner is very effective with the MLFMA and can reduce both the iteration number and the computational time significantly.Index Terms-Electromagnetic scattering, generalized minimal residual (GMRES) method, multilevel fast multipole algorithm (MLFMA), preconditioning techniques.