Application of a tyrosinase microreactor – detector in a flow injection configuration for the determination of affinity and dynamics of inhibitor binding

Vandeput, Marie; Patris, Stéphanie; Silva, Hugo; Parsajoo, Cobra; Dejaeghere, Bieke; Martinez, Julia Arcos; Kauffmann, Jean‐Michel

doi:10.1016/j.snb.2017.03.156

Cited by 16 publications

(11 citation statements)

References 61 publications

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“…As expected from these observations, the results of the paired t-test (p < 0.01) revealed that the immobilized tyrosinase was more stable during storage than the free tyrosinase solution. In addition, when compared to the stability results of tyrosinase immobilized using a range of methods (see Table 2), these data confirm that the immobilization method used herein is superior to previously reported methods (16)(17)(18)(19)). Regression-plot repeatability was determined using TYR-MNPs from batch 1, the results of which are listed in Table 3.…”

Section: Tyr-mnp Reusability and Stabilitysupporting

confidence: 84%

Green and efficient determination of ʟ-dopa in complex polypill formulations using a magnetic microplate

Tsai

Chen

et al. 2018

Green Chemistry Letters and Reviews

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A high-throughput and selective method for the determination of ʟ-dopa (levodopa) in complex formulations was developed. The method is based on the oxidation of ʟ-dopa to yield dopachrome using tyrosinase-labeled magnetic nanoparticles (TYR-MNPs) as the oxidation catalyst. TYR-MNP activity was retained at 75% after 20 reuse cycles, which is superior to previously reported systems that employ other substrates or cross-linkers for the immobilization of tyrosinase. In addition, the precision (< 3%), accuracy (recovery = 95-102%), and selectivity of the newly developed quantitative-analysis method for ʟ-dopa in complex polypill formulations meets the pharmaceutical industry's quality-control requirements; consequently this method can be applied to the routine analysis of complex formulations. The quality-control assay uses 96well microplates, which reduces the required volume of reagents, and the tyrosinase can easily be recycled and reused using an in-house-prepared magnetic microplate, thereby rendering the proposed method economical and less wasteful than existing methods.

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Section: Tyr-mnp Reusability and Stabilitysupporting

confidence: 84%

Green and efficient determination of ʟ-dopa in complex polypill formulations using a magnetic microplate

Tsai

Chen

et al. 2018

Green Chemistry Letters and Reviews

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“…As an example, a colorimetric assay based on a combination of chitosan-stabilized platinum nanoparticels, catechol, tyrosinase, and 3,3',5,5'-tetramethylbenzidine as the probe being oxidized to a yellow diamine has been proposed [221]. A microreactor-detector based on the use of a gold disk for tyrosinase immobilization and a carbon disk as a working electrode able to amperometrically detect dopaquinone formation starting from l-tyrosine has also been described [222]. Immobilized tyrosinase has been used also coupled to capillary electrophoresis [223,224].…”

Section: New Trends In the Search For Tyrosinase Inhibitorsmentioning

confidence: 99%

Natural and Bioinspired Phenolic Compounds as Tyrosinase Inhibitors for the Treatment of Skin Hyperpigmentation: Recent Advances

2019

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One of the most common approaches for control of skin pigmentation involves the inhibition of tyrosinase, a copper-containing enzyme which catalyzes the key steps of melanogenesis. This review focuses on the tyrosinase inhibition properties of a series of natural and synthetic, bioinspired phenolic compounds that have appeared in the literature in the last five years. Both mushroom and human tyrosinase inhibitors have been considered. Among the first class, flavonoids, in particular chalcones, occupy a prominent role as natural inhibitors, followed by hydroxystilbenes (mainly resveratrol derivatives). A series of more complex phenolic compounds from a variety of sources, first of all belonging to the Moraceae family, have also been described as potent tyrosinase inhibitors. As to the synthetic compounds, hydroxycinnamic acids and chalcones again appear as the most exploited scaffolds. Several inhibition mechanisms have been reported for the described inhibitors, pointing to copper chelating and/or hydrophobic moieties as key structural requirements to achieve good inhibition properties. Emerging trends in the search for novel skin depigmenting agents, including the development of assays that could distinguish between inhibitors and potentially toxic substrates of the enzyme as well as of formulations aimed at improving the bioavailability and hence the effectiveness of well-known inhibitors, have also been addressed.

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“…In other words, sensitive and correct assay methods for screening and development of effective tyrosinase inhibitors are of great importance. For this purpose, several spectrophotometric 6–10 , chromatographic 11–17 , electrophoretic 18–22 , radiometric 23 , 24 and electrochemical 25–27 assays have been applied and developed by researchers so far. Recently, a novel fluorescent biosensor 28 and tyrosinase-based thin-layer chromatography-autography have been suggested for tyrosinase inhibitor screening 29 .…”

Section: Introductionmentioning

confidence: 99%

A comprehensive review on tyrosinase inhibitors

Zolghadri

Bahrami

Khan³

et al. 2019

Journal of Enzyme Inhibition and Medicinal Chemistry

774

471

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Tyrosinase is a multi-copper enzyme which is widely distributed in different organisms and plays an important role in the melanogenesis and enzymatic browning. Therefore, its inhibitors can be attractive in cosmetics and medicinal industries as depigmentation agents and also in food and agriculture industries as antibrowning compounds. For this purpose, many natural, semi-synthetic and synthetic inhibitors have been developed by different screening methods to date. This review has focused on the tyrosinase inhibitors discovered from all sources and biochemically characterised in the last four decades.

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Application of a tyrosinase microreactor – detector in a flow injection configuration for the determination of affinity and dynamics of inhibitor binding

Cited by 16 publications

References 61 publications

Green and efficient determination of ʟ-dopa in complex polypill formulations using a magnetic microplate

Green and efficient determination of ʟ-dopa in complex polypill formulations using a magnetic microplate

Natural and Bioinspired Phenolic Compounds as Tyrosinase Inhibitors for the Treatment of Skin Hyperpigmentation: Recent Advances

A comprehensive review on tyrosinase inhibitors

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