Application of a Validated Method for the Determination of Provitamin A Carotenoids in Indonesian Foods of Different Maturity and Origin

Hulshof, P.J.M.; Xu, Chunfu; Bovenkamp, P. van de; Muhilal,; West, C.E.

doi:10.1021/jf9603137

Cited by 74 publications

(60 citation statements)

References 19 publications

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“…High levels of β-carotene in Sauropus androgynus leavesellow color of shank in broiler chickens fed diets supplemented with SALE. Hulshof et al (1997) reported that Sauropus androgynus leaves contained as much carotene 12.657 µg per 100 g of fresh leaves (Hulshof et al, 1997). plus 0 g SALE; P1 = 19% protein plus 4.5 g SALE/kg diet; P2 = 17% protein plus 4.5 g SALE /kg; P3 = 15% protein plus 4.5 g SALE/kg; P4 = 19% protein plus 9 g SALE/kg; P5 = 17% protein plus 9 g SALE /kg; P6 = 15% protein plus 9 g SALE/ kg…”

Section: Carcass Qualitymentioning

confidence: 99%

Effect of Sauropus Androgynus Leaves Extract (Sale) on Fat Deposition in Broiler Fed Low Protein Diets

Santoso

Kususiyah

Fenita

2013

J. Indonesian Trop. Anim. Agric.

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ABSTRAKPenelitian ini dirancang untuk mengevaluasi efektivitas ekstrak daun katuk (EDK) untuk menurunkan penimbunan lemak pada broiler yang diberi pakan berprotein rendah. Delapan puluh empat broiler jantan dan betina (1:1) umur 20 hari didistribusikan ke dalam 7 kelompok perlakuan sebagai berikut: 1) Broiler dengan pakan mengandung 19% protein tanpa ekstrak daun katuk (EDK)/kg (P0); 2) Broiler dengan pakan mengandung 19% protein plus 4,5 g EDK/kg (P1); 3) Broiler dengan pakan mengandung 17% protein plus 4,5 g EDK/kg (P2); 4) Broiler dengan pakan mengandung 15% protein plus 4,5 g EDK/kg (P3); 5) Broiler dengan pakan mengandung 19% protein plus 9 g EDK/kg (P4); 6) Broiler dengan pakan mengandung 17% protein plus 9 g EDK/kg (P5); 7) Broiler dengan pakan mengandung 15% protein plus 9 g EDK/kg (P6). Hasil penelitian menunjukkan bahwa suplementasi ekstrak daun katuk dalam pakan berprotein rendah berpengaruh tidak nyata terhadap warna daging, cacat dada, bau karkas, dan fatty liver score (P>0,05) tetapi berpengaruh nyata terhadap cacat paha (P<0,01), warna kaki, rasa dan bau daging (P<0,05). Selain itu, suplementasi ekstrak daun katuk berpengaruh nyata terhadap kadar lemak (P<0,05) dan sangat nyata terhadap kadar kolesterol daging (P<0,01), tetapi berpengaruh tidak nyata (P>0,05) terhadap kadar protein dan komposisi asam lemak daging. Disimpulkan bahwa suplementasi ekstrak daun katuk pada dosis 9 g/kg pakan mampu menormalkan deposisi lemak pada broiler yang diberi pakan berprotein rendah. Ekstrak katuk juga mampu memperbaiki mutu karkas broiler. Kata kunci: esktrak daun katuk, penimbunan lemak, protein rendah, broiler ABSTRACTThe present study was designed to evaluate the effect of Sauropus Androgynus Leaves Extract (SALE) on broiler chickens fed low protein diets. Eighty four mixed broiler chickens (male:female = 1:1) aged 20 days were distributed to seven treatment groups as follows: 1) Broilers fed 19% protein without SALE (P0); 2) Broilers fed 19% protein plus 4.5 g SALE/kg (P1); 3) Broilers fed 17% protein plus 4,5 g SALE/kg (P2); 4) Broilers fed 15% protein plus 4.5 g SALE/kg (P3); 5) Broilers fed 19% protein plus 9 g SALE/kg (P4); 6) Broilers fed 17% protein plus 9 g SALE/kg (P5); 7) Broilers fed 15% protein plus 9 g SALE/kg (P6). Experimental results showed that supplementation of Sauropus androgynus extract to low protein diets had no effect on meat colour, haemorrhages scale in breast meat, and fatty liver score (P>0.05), but it significantly affected haemorrhages scale in thigh meat (P<0,01), shank colour, meat taste and flavour (P<0.05). In addition, supplementation of the extract significantly affected meat fat content (P<0.05) and meat cholesterol content (P<0.01), but it had no effect on meat protein content (P>0.05). It can be concluded that the leaves extract of Sauropus androgynus supplementation at level of 9 g/kg diets normalized fat deposition in broiler chickens fed low protein diets. SALE improved broiler meat qualities.

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Section: Carcass Qualitymentioning

confidence: 99%

Effect of Sauropus Androgynus Leaves Extract (Sale) on Fat Deposition in Broiler Fed Low Protein Diets

Santoso

Kususiyah

Fenita

2013

J. Indonesian Trop. Anim. Agric.

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“…On each occasion, portions were collected for analysis over 3 consecutive days, which corresponded to the 3-d menu cycle. Duplicate samples of the meals were pooled per treatment group per 3 consecutive days, frozen at Ҁ20°C, and later shipped (in dry ice) to the Division of Human Nutrition and Epidemiology for analyses of fat, protein, dietary fiber, ash, dry matter, iron, retinol, and carotenoids (20,21). Carbohydrate content was calculated by the difference from the analyzed content of the above substances.…”

Section: Duplicate Food Portionsmentioning

confidence: 99%

The contribution of plant foods to the vitamin A supply of lactating women in Vietnam: a randomized controlled trial

Khan

West

Pee

et al. 2007

The American Journal of Clinical Nutrition

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Background: More information is needed on the efficacy of carotenoids from plant foods in improving vitamin A status. Objective: We aimed to quantify the efficacy of provitamin A-rich vegetables and fruit in improving vitamin A status. Design: Breastfeeding women in 9 rural communes in Vietnam were randomly allocated to 1 of 4 groups: the vegetable group (n ҃ 73), which ingested 5.6 mg ␤-carotene/d from green leafy vegetables; the fruit group (n ҃ 69), which ingested 4.8 mg ␤-carotene/d from orange or yellow fruit; the retinol-rich group (n ҃ 70), which ingested 610 g retinol/d from animal foods and 0.6 mg ␤-carotene/d; and the control group (n ҃ 68), which ingested 0.4 mg ␤-carotene/d. Meals of groups 1, 2, and 4 contained 30 g retinol/d. Lunch and dinner were provided 6 d/wk for 10 wk. Results: Mean (95% CI) changes in serum retinol concentrations of the vegetable, fruit, retinol-rich, and control groups were 0.09 (0.03, 0.16), 0.13 (0.07, 0.19), 0.25 (0.17, 0.33), and 0.00 (Ҁ0.06, 0.06) mol/L, respectively. Mean (95% CI) changes in breast-milk retinol concentrations were 0.15 (0.04, 0.27), 0.15 (0.02, 0.28), 0.48 (0.32, 0.64), and Ҁ0.06 (Ҁ0.21, 0.09) mol/L, respectively. According to these findings, the equivalent of 1 g retinol would be 12 g ␤-carotene (95% CI: 8, 22 g) for fruit and 28 g ␤-carotene (17, 84 g) for green leafy vegetables. Thus, apparent mean vitamin A activity of carotenoids in fruit and in leafy vegetables was 50% (95% CI: 27%, 75%) and 21% (7%, 35%), respectively, of that assumed. Conclusion:The bioavailability of carotenoids from vegetables and fruit is less than previously assumed.Am J Clin Nutr 2007;85: 1112-20.

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“…The limit of detection (LOD), calculated as LOQ divided by 2.5 (Hulshof et al, 1997) was 0.0156 μg/mL. The precision of the instrumental technique (n=10) was evaluated by analyzing standard samples prepared by serial dilution within the working range each day for about two weeks.…”

Section: Validation Of the Established Methods Linearity And Calibratimentioning

confidence: 99%

“…This was performed six times (n=6). The limit of detection (LOD) defined as amount of the carotenoid, resulting in a peak height three times the baseline noise (Hulshof et al 1997) was calculated.…”

Section: Peak Identification and Quantificationmentioning

confidence: 99%

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HPLC Method Optimization and Validation for Determination of Lycopene in Tomato (<i>Lycopersicon esculentum</i>, Mill.) Fruits

Berra¹

2013

Sci. Technol. Arts Res. J.

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Article InformationThe aim of this work was to develop efficient and accurate protocol that can measure lycopene in tomato. A total of ten tomato varieties loose and cluster types were selected for the study. All the tomatoes were harvested at commercial ripening stage and prepared after 3 days of storage at 18°C and relative humidity (RH) of 80%. For analysis purpose each tomato was cut, quartered, chopped and frozen in liquid nitrogen and then subjected to grinding. The finely ground tomato samples were immediately filled into air-tight plastic tube and stored in freezer (-80 °C) for about two weeks. Finally, the tomato samples were prepared in different composition of extraction solvent before subjected to HPLC analysis. Mobile phase composition Acetonitrile/Methanol (50:50, v/v) added with Triethylamine 9 μM; extraction solvents hexane/acetone/ethanol (50:25:25 v/v/v); re-dissolving residue in Tetrahydrofuran, followed Acetonitrile/Methanol (15:30:55, v/v/v); flow rate 0.6 mL/min and λdetection near to 472nm were showed most suitable for lycopene determination in tomato, achieving best characteristic spectral profile with precision (RSD<15), accuracy and recovery (≥81.7%), and sensitive detection limit (0.0156 μg/mL) within separation of ~21 minutes. The result showed that HPLC is most accepted and efficient method for determining lycopene in tomato.

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Application of a Validated Method for the Determination of Provitamin A Carotenoids in Indonesian Foods of Different Maturity and Origin

Cited by 74 publications

References 19 publications

Effect of Sauropus Androgynus Leaves Extract (Sale) on Fat Deposition in Broiler Fed Low Protein Diets

Effect of Sauropus Androgynus Leaves Extract (Sale) on Fat Deposition in Broiler Fed Low Protein Diets

The contribution of plant foods to the vitamin A supply of lactating women in Vietnam: a randomized controlled trial

HPLC Method Optimization and Validation for Determination of Lycopene in Tomato (<i>Lycopersicon esculentum</i>, Mill.) Fruits

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