Application of a zona pellucida binding assay (ZBA) in the domestic cat benefits from the use of in vitro matured oocytes

Hermansson, Ulrika; Axnér, Eva; Holst, Bodil Ström

doi:10.1186/1751-0147-49-28

Cited by 8 publications

(7 citation statements)

References 35 publications

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“…Our data confirm previous findings that porcine immature and in vitro-matured oocytes have a more porous net-like structure than the more compact surface of in vivo-matured oocytes [88]. Similarly, some studies showed that oocyte maturation is accompanied by changes in the ZP structure in human [30], mouse [31], and cat [32]. These changes may be relevant in the process of binding and penetration of spermatozoa into the ZP.…”

Section: Discussionsupporting

confidence: 91%

“…Therefore, we compared the composition and structure of the equine and porcine ZP as follows: 1) we identified the glycoproteins that comprise the equine and porcine ZP, 2) we described their localization in the ZP in both species using confocal fluorescence microscopy, and 3) we analyzed the morphological structure of the equine and porcine ZP using transmission and scanning electron microscopy. Moreover, earlier studies [30][31][32] have indicated that the ZP could be modified during maturation. Therefore, we analyzed immature, in vitro-matured, and in vivo-matured oocytes.…”

Section: Introductionmentioning

confidence: 99%

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New Insights into the Mechanisms of Fertilization: Comparison of the Fertilization Steps, Composition, and Structure of the Zona Pellucida Between Horses and Pigs1

Mugnier

Dell’Aquila

Peláez

et al. 2009

Biology of Reproduction

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The mechanism of fertilization remains largely enigmatic in mammals. Most studies exploring the molecular mechanism underlying fertilization have been restricted to a single species, generally the mouse, without a comparative approach. However, the identification of divergences between species could allow us to highlight key components in the mechanism of fertilization. In the pig, in vitro fertilization (IVF) and polyspermy rates are high, and spermatozoa penetrate easily through the zona pellucida (ZP). In contrast, IVF rates are low in the horse, and polyspermy is scarce. Our objective was to develop a comparative strategy between these two divergent models. First, we compared the role of equine and porcine gametes in the following five functions using intraspecific and interspecific IVF: ZP binding, acrosome reaction, penetration through the ZP, gamete fusion, and pronucleus formation. Under in vitro conditions, we showed that the ZP is a determining element in sperm-ZP attachment and penetration, whereas the capacity of the spermatozoa is of less importance. In contrast, the capacity of the spermatozoa is a key component of the acrosome reaction step. Second, we compared the composition and structure of the equine and porcine ZP. We observed differences in the number and localization of the ZP glycoproteins and in the mesh-like structure of the ZP between equine and porcine species. These differences might correlate with the differences in spermatozoal attachment and penetration rates. In conclusion, our comparative approach allows us to identify determining elements in the mechanism of fertilization.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

New Insights into the Mechanisms of Fertilization: Comparison of the Fertilization Steps, Composition, and Structure of the Zona Pellucida Between Horses and Pigs1

Mugnier

Dell’Aquila

Peláez

et al. 2009

Biology of Reproduction

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“…Alternatively, in vitro redefinition of the approach resulted in more conflicting outcomes. In our study the use of fresh in vitro matured oocytes was adopted, as the beneficial use of IVM oocytes for application of ZP-binding assay has been reported in different species (Marco-Jiménez & Vicente 2004;Hermansson et al, 2007) as also supported by the fact that mature and immature oocytes differ in the glycoproteins of the zona pellucida, along with the number of sperm receptors (Lucas et al, 2003). By contrast Braundmeier and colleagues (2002) in vitro competitive zona binding assay could rank the bulls, but did not correlate with the 56day no return rates, zona binding in vivo, or the number of accessory spermatozoa detected on in vivo produced embryos.…”

Section: Discussionmentioning

confidence: 99%

In vitrocompetitive binding index using fluorochrome-labelled spermatozoa for predicting bull fertility

Puglisi

Krvavac

Bonacina

et al. 2010

Zygote

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This work evaluated if an in vitro test, with the combined power of the statistical evaluation of spermatozoa and zona pellucida (ZP) competitive binding ability and a rapid method for accessory sperm counts, could predict the bull fertility. Ten Holstein Friesian bulls of known field fertility (five of high and five of low fertility) were selected. An in vitro heterospermic insemination approach, based on differential staining, was tested on 45 possible pairs of bulls (two batches per bull). Motility and quality (abnormalities and membrane status) seminal characteristics and estimated relative conception rates (ERCR) highlighted only one association between membrane integrity and ERCR (p = 0.007). Differences in ZP binding allowed us to rank bulls into two categories based on low and high binding ability. For eight bulls, this classification reflected the ERCR. Differences between batches were reported for two bulls, in which the effect of heterospermic insemination (the number of sperm binding to ZP from different bulls not in a 1:1 ratio) showed a significant bull-related effect (p < 0.001) in the first batch and no effect (p > 0.05) in the second batch for both bulls. Reduction of the number of oocytes per assay from 25 to 5 had no effect (p > 0.5) on the bulls' ranking. Our results suggest that in vitro competitive binding is a promising approach for estimating bull fertility and support concepts for further implementation, e.g. drastic reduction of oocyte number in a single pair assay and larger scale testing for batches.

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“…ZBA has been studied in dogs (Hermansson, Ponglowhapan, Forsberg, & Holst, ) as well as in cats (Hermansson, Axnér, & Holst, ). The interaction between spermatozoa and the ZP is a critical event resulting in fertilization and reflects multiple sperm functions (i.e.…”

Section: Review Of Sperm Functional Testsmentioning

confidence: 99%

“…ZBA has been studied in dogs (Hermansson, Ponglowhapan, Forsberg, & Holst, 2006) as well as in cats (Hermansson, Axnér, & Holst, 2007).…”

Section: Zona Pellucida Binding Assay (Zba)mentioning

confidence: 99%

Evaluation of spermatozoal function—useful tools or just science

Niżański

Partyka

Prochowska

2016

Reprod Domestic Animals

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Contents Conventional microscopic semen analysis does not provide precise information on the fertilizing potential of a male. The traditional basis for semen evaluation is that male fertility is dependent on production of a “proper” concentration/number of motile, morphologically normal spermatozoa in excess to achieve conception. Many independent studies, especially in human medicine, have demonstrated that the absolute number of spermatozoa does not accurately determine fertility, but their functional competence. Many functional tests of spermatozoa are developed over the last decades. Computer‐assisted sperm analysis (CASA) and flow cytometry have become the gold standard for semen assessment in specialized andrology laboratories. Other functional assays, such as gamete interaction tests, provide additional information regarding the real fertilizing potential of sperm cells. From this point of view, such tests are valuable diagnostic tools in fertility disorders and may be helpful to make a decision which method of treatment to use: pharmacological therapy, intrauterine insemination, introduction of classic IVF, ICSI or exclusion from a breeding programme. The most useful gamete interaction tests include induced acrosome reaction, zona pellucida binding assay, oocyte penetration assay and hyaluronan binding assay. In recent years, andrology has entered into a new era of sophisticated OMICS methods. Genomics, epigenomics, transcriptomics and proteomics brought high hopes for rapid progress in clinical diagnostics.

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Application of a zona pellucida binding assay (ZBA) in the domestic cat benefits from the use of in vitro matured oocytes

Cited by 8 publications

References 35 publications

New Insights into the Mechanisms of Fertilization: Comparison of the Fertilization Steps, Composition, and Structure of the Zona Pellucida Between Horses and Pigs1

New Insights into the Mechanisms of Fertilization: Comparison of the Fertilization Steps, Composition, and Structure of the Zona Pellucida Between Horses and Pigs1

In vitrocompetitive binding index using fluorochrome-labelled spermatozoa for predicting bull fertility

Evaluation of spermatozoal function—useful tools or just science

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