Application of adipose tissue-derived stem cells in a rat rotator cuff repair model

Mora, Maria Valencia; Antuña, Samuel; Arranz, Mariano García; Carrascal, M. T.; Barco, Raúl

doi:10.1016/s0020-1383(14)70006-3

Cited by 74 publications

(53 citation statements)

References 46 publications

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“…BMSCs are known for their high differentiation potential and low morbidity during harvesting. In 2001, ASCs were first identified as MSCs from adipose tissue [29] and since then increasing studies indicated that ASCs might be suitable as seeding cells for tissue engineering and regenerative medicine [30]. ASCs and BMSCs share common characteristics, including growth and morphology [31], displaying fibroblastic features, and a similar transcriptional and cell surface marker expression profile [32].…”

Section: Discussionmentioning

confidence: 99%

Estrogen Receptor α and β in Mouse: Adipose-Derived Stem Cell Proliferation, Migration, and Brown Adipogenesis In Vitro

Zhang

Schmull

et al. 2016

Cell Physiol Biochem

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Background/Aims: Adipose-derived stem cells (ASCs) belong to mesenchymal stem cells and may play a potential role as seeding cells in stem cell transplantation. To be able to exploit stem cells as therapeutic tool, their defects in some important cellular functions, such as low survival rate and cellular activity, should be considered. This is especially the case for stem cells that are intended for transplantation. Of note, stem cell responses to hormones should be considered since estrogen is known to play a critical role in stem cell behavior. However, different impacts of the estrogen receptor (ER) types α and β have not been fully determined in ASC function. In this study, we investigated effects of ERα and ERβ on ASC proliferation, migration, as well as in adipogenesis. Methods: ASCs obtained from mice were cultured with 100nM ERα or ERβ agonist PPT and DPN, respectively. The ERα and ERβ antagonist ICI 182,780 (100nM) was used as control. Results: Compared to ERβ, ERα appears more potent in improving ASC proliferation and migration. Investigation of adipogenesis revealed that ERβ played a significant role in suppressing ASC-mediated brown tissue adipogenesis which is in contrast to ERα. These results correlated with reduced mRNA expression of UCP-1, PGC-1α and PPAR-γ. Conclusions: ERα plays a more critical role in promoting ASC proliferation and migration while ERβ is more potent in suppressing ASC brown adipose tissue differentiation mediated by decreased UCP-1, PGC-1α and PPAR-γ expression.

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Section: Discussionmentioning

confidence: 99%

Estrogen Receptor α and β in Mouse: Adipose-Derived Stem Cell Proliferation, Migration, and Brown Adipogenesis In Vitro

Zhang

Schmull

et al. 2016

Cell Physiol Biochem

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“…On one hand, the increased multipotency of EM ASCs and their unique effects on myogenic cells in culture suggest that they might be a more promising stem cell source than the previously evaluated s.c. ASCs for improving cuff healing [20][21][22]. On the other hand, the beige characteristics of EM fat suggest that they could be "browned," which is associated with the secretion of various growth factors upstream of the muscle repair and hypertrophy pathways [19,46,47].…”

Section: Therapeutic Implicationsmentioning

confidence: 99%

“…Recent research has focused on anatomically distant white fat depots as a potential source of ASCs [20][21][22] for promoting rotator cuff healing. We hypothesized that epimuscular fat might represent a unique beige depot with therapeutic promise either as an autologous source of ASCs or as a modular source of paracrine myogenic signaling.…”

Section: Introductionmentioning

confidence: 99%

Epimuscular Fat in the Human Rotator Cuff Is a Novel Beige Depot

Meyer

Gibbons

Sato

et al. 2015

Stem Cells Translational Medicine

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Chronic rotator cuff (RC) tears are a common and debilitating injury, characterized by dramatic expansion of adipose tissue, muscle atrophy, and limited functional recovery. The role of adipose expansion in RC pathology is unknown; however, given the identified paracrine/endocrine regulation by other adipose depots, it likely affects tissue function outside its boundaries. Therefore, we characterized the epimuscular (EM) fat depot of the human rotator cuff, defined its response to RC tears, and evaluated its influence on myogenesis in vitro. EM fat biopsies exhibited morphological and functional features of human beige fat compared with patient-matched s.c. biopsies, which appeared whiter. The transcriptional profile of EM fat and isolated EM adipose-derived stem cells (ASCs) shifted as a function of the tear state; EM fat from intact cuffs had significantly elevated expression of the genes associated with uncoupled respiration, and the EM fat from torn cuffs had increased expression of beige-selective genes. EM ASC cocultures with human-and mouse-derived myogenic cells exhibited increased levels of myogenesis compared with s.c. cultures. Increased fusion and decreased proliferation of myogenic cells, rather than changes to the ASCs, were found to underlie this effect. Taken together, these data suggest that EM fat in the human rotator cuff is a novel beige adipose depot influenced by cuff state with therapeutic potential for promoting myogenesis in neighboring musculature. STEM CELLS TRANSLATIONAL MEDICINE 2015;4:764-774 SIGNIFICANCERotator cuff tears affect millions of people in the U.S.; however, current interventions are hindered by persistent muscle degeneration. This study identifies the therapeutic potential for muscle recovery in the epimuscular fat in the rotator cuff, previously considered a negative feature of the pathology, and finds that this fat is beige, rather than white. This is important for two reasons. First, the stem cells that were isolated from this beige fat are more myogenic than those from white fat, which have been the focus of stem cell-based therapies to date, suggesting epimuscular fat could be a better stem cell source to augment rotator cuff repair. Second, these beige stem cells promote myogenesis in neighboring cells in culture, suggesting the potential for this fat to be manipulated therapeutically to promote muscle recovery through secreted signals.

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“…Electrochemically aligned collagen fiber and skin substrate have been coupled with stem cells for both tendon [26] and skin regeneration [27], respectively. Collagen carrier/gel [28] or collagen combined with stem cell accelerated the wound healing in healing-impaired db/db mice [29].…”

Section: Collagenmentioning

confidence: 99%

Biomaterials for Tendon/Ligament and Skin Regeneration

Cheng¹

2018

Biomaterials in Regenerative Medicine

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Tendon/ligament injury or skin injuries due to diseases, trauma, and surgery are common. Timely functional repair and tissue regeneration is a key to improve the quality of life of the patient while reducing health care cost. Tendon/ligament/skin is also enriched in a common extracellular matrix (ECM), collagen I, III, and elastin. Tissue engineering and regenerative medicine, the combination of (stem) cells, growth factors, and biomaterial scaffolds, is an emergent field, which has attracted substantial attention over the years. Biomaterials are considered the foundation of regenerative medicine. A key to find a new solution to tendon/ligament/skin healing is to synthesize new functional biomaterials, which have better biomechanical properties, biodegradability, and cell supporting properties. This chapter will review existing FDA-approved biomaterial-based therapy, as well as those in development.

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Application of adipose tissue-derived stem cells in a rat rotator cuff repair model

Cited by 74 publications

References 46 publications

Estrogen Receptor α and β in Mouse: Adipose-Derived Stem Cell Proliferation, Migration, and Brown Adipogenesis In Vitro

Estrogen Receptor α and β in Mouse: Adipose-Derived Stem Cell Proliferation, Migration, and Brown Adipogenesis In Vitro

Epimuscular Fat in the Human Rotator Cuff Is a Novel Beige Depot

Biomaterials for Tendon/Ligament and Skin Regeneration

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