Application of Agrobacterium Rol Genes in Plant Biotechnology: A Natural Phenomenon of Secondary Metabolism Regulation

Bulgakov, Victor P.; Shkryl, Yuri N.; Veremeichik, Galina N.; Gorpenchenko, Tatiana Y.; Inyushkina, Yuliya V.

doi:10.5772/24200

Cited by 5 publications

(5 citation statements)

References 28 publications

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“…Alternatively, their leaves have 2 times more 3 carotenoids per mass index and to 10% less flavonoids than the leaves of non-transformed D.purpurea plants. However, at the end of cultivation, the differences between all three types of metabolites "change their sign" that is explained with the expression of rol-genes in the cells of the transformed regenerants [2]. Figure 2 indicates that the radiostimulation of flavonoid synthesis exists only under radiation exposure of Ri-transformed regenerants of D. purpurea with stress-causative doses 2 and 5 Gy (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies showed that the plants obtained through regeneration from hairy roots, which had previously been transformed with a virulent strain of Agrobacterium rhizogenes, had the set of necessary properties [1; 2]. Such Ri-transformed regenerated plants were characterized with the increasing synthesis of the secondary metabolites due to the integration of Ri-plasmid T-DNA rol-genes of Agrobacterium into the plant genome with their next activation and gene expression [2]. However, in the case of D. purpurea, the synthesis of particular metabolites, cardioglycosides, has to be stimulated.…”

Section: Introductionmentioning

confidence: 99%

“…The latter depends on the photosystems' state and the ratio of the total chlorophyll a and b content. At the same time, it is known that the expression of the rolC gene in the transformed plant cells reduces the concentration of chlorophyll primarily due to form a, which is necessary for efficient photosynthesis [2]. We have proposed to solve this problem by using the low-ionizing radiation exposure of Digitalis regenerants that would increase the chlorophyll content in the stimulation dose-range [4; 5].…”

Section: Introductionmentioning

confidence: 99%

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X-Ray Effects on Stress Response of the Ri-Transformants in Vitro Digitalis Purpurea L

Lioshina¹,

Bulko²,

Litvinov³

et al. 2017

RadJ

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Abstract. Digitalis purpurea L. is the source of a number of biologically active substances used for medical purposes. Agrobacterium rhizogenes-transformed plants show changes in the secondary metabolism. The influence of acute exposure to ionizing radiation on the modification of the pigment system and accumulation of flavonoids in Ritransformed regenerated plants in vitro was studied. A week after the exposure to the stressor, changes in the number of chlorophylls, carotenoids and flavonoids were observed. A month later, the amount of carotenoids and the ratio of chlorophyll a/b decreased, which indicated a decrease in the photosynthetic capacity of the irradiated plants. The synthesis of flavonoids was reduced as well. But in the second passage, we saw an increase in all the parameters studied in relation to the non-irradiated samples. It showed the development of a long-acting adaptive response of plants. A nonlinear dose dependence of acute X-ray irradiation influence on in vitro D. purpurea was shown. The transformed plants were found to be more resistant to the damaging effects of radiation. We reasoned that the exposure to ionizing radiation in a certain dose interval could be used to stimulate the accumulation of biologically active substances of in vitro D. purpurea.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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X-Ray Effects on Stress Response of the Ri-Transformants in Vitro Digitalis Purpurea L

Lioshina¹,

Bulko²,

Litvinov³

et al. 2017

RadJ

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“…Агробактеріальна трансформація наперстянки пурпурової, імовірно, модифікує регуляцію біосинтезу флавоноїдів і трансформанти по-іншому реагують на окислювальний стрес [15], індукований радіацією. Біохімічна адаптація у відповідь на дію іонізуючого опромінення у таких рослинах не виражена.…”

Section: результати та обговоренняunclassified

Changes in the content of carotenoids and flavonoids in medicinal raw material of Digitalis purpurea, Polemonium caeruleum, and Aerva lanata, cultivated in vitro under the chronic action of ionizing radiation

Litvinov¹,

Lioshyna²,

Bulko³

et al. 2021

Nucl. Phys. At. Energy

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Chronic low-dose γ-irradiation of Digitalis purpurea and Aerva lanata plants in vitro have led to an increase in the content of carotenoids and flavonoids in the leaves at the beginning of the post-radiation period. In the following, the content of pigments and flavonoids decreased in comparison to the control samples. For Polemonium caeruleum such changes had been not observed. The qualitative composition of the secondary metabolites has not been changed, but the ratio of the individual components of the extract varied within a small range. Ri-transformants of Digitalis purpurea have been shown smaller changes in response to irradiation. We assume that the observed effects are the manifestation of the adaptive response of plants to oxidative stress caused by chronic low-dose irradiation.

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“…Antibacterial is a substance that can inhibit the growth and kill pathogenic bacteria (Paju et al, 2013). Testing was carried out to determine the minimum inhibitory concentration value to increase the effectiveness of the antibacterial compound as well as prevent the emergence of bacterial resistance problems because the administration of antibacterials in excessive amounts and continuously will cause bacterial cells to become resistant (Kuspradini et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Effectiveness Test of Ethanol Extract of Gadung Mango Leaves Against Staphylococcus aureus and Escherichia coli Bacteria

Indira Pipit Miranti,

Vivin Andriani

2024

Quagga

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Infection is a disease that often occurs in Indonesia. This disease is caused by pathogenic microbes, one of which is bacteria. Staphylococcus aureus and Escherichia coli are bacteria that cause infection. Pharmacological therapy for bacterial infections used to use antibiotics, currently tends to use drugs that come from nature. One of the plants that has the potential as an antibacterial is gadung mango leaves. This study aims to determine the effectiveness of mango leaf ethanol extract against S.aureus and E.coli bacteria. The method used was well diffusion with concentrations of ethanol extract of mango leaves 0.5% (S1), 0.75% (S2), 1% (S3), streptomycin (K+), and ethanol (K-), respectively treatment was repeated 5 times. Data that can be analyzed using One Way ANOVA with a significant level of 0.05. Based on the results of the antibacterial extract of ethanol extract of mango gadung leaves, it has the potential to be better on E. coli bacteria, namely at a concentration of 1% (S3) of 16.05 mm, whereas on S.aureus K+ bacteria (Streptomycin) it is better on control + of 15.9 mm.

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Application of Agrobacterium Rol Genes in Plant Biotechnology: A Natural Phenomenon of Secondary Metabolism Regulation

Cited by 5 publications

References 28 publications

X-Ray Effects on Stress Response of the Ri-Transformants in Vitro Digitalis Purpurea L

X-Ray Effects on Stress Response of the Ri-Transformants in Vitro Digitalis Purpurea L

Changes in the content of carotenoids and flavonoids in medicinal raw material of Digitalis purpurea, Polemonium caeruleum, and Aerva lanata, cultivated in vitro under the chronic action of ionizing radiation

Antibacterial Effectiveness Test of Ethanol Extract of Gadung Mango Leaves Against Staphylococcus aureus and Escherichia coli Bacteria

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