Application of an antimicrobial packaging material from chicken bone gelatine and cinnamon bark oil to mozzarella cheese

Abstract

Summary Chicken bones are mostly discarded even though they contain a considerable amount of proteins, which would allow them to be applicable as a protein film source. In this study, chicken bone gelatine (CBG) films were prepared and characterised after the addition of cinnamon bark oil (CBO) to confer antimicrobial activity. The incorporation of 1% CBO increased the tensile strength of the CBG film from 18.2 to 23.9 MPa, whereas elongation at break was decreased from 15.6% to 5.91% and water solubility from 88.9% to 62.2%. Antimicrobial activity of the CBG films against foodborne pathogens as well as their antioxidant activity increased with the increase in CBO concentration. When CBG film containing 1% CBO was used for wrapping mozzarella cheese inoculated with Listeria monocytogenes, the population of the bacterium decreased after 20 days in storage, indicating that CBG films can be applied for improving the microbiological safety of mozzarella cheese.

Development of Burdock Root Inulin/Chitosan Blend Films Containing Oregano and Thyme Essential Oils

“…The antimicrobial activities of the films containing OT with different concentrations against the four common pathogens were determined using the disc diffusion assay described by Kim et al [ 45 ]. L. monocytogenes (KCTC 13064, ATCC 15313), S. aureus (KCTC 1621, ATCC 10537), E. coli O157:H7 (ATCC 43889, NCTC 12079), and S. typhimurium (ATCC 14028, KCTC 2421) inocula at concentrations of 6–7 log CFU/mL were spread onto the surface of Mueller Hinton Agar media.…”

Sea Squirt Shell Protein and Polylactic Acid Laminated Films Containing Cinnamon Bark Essential Oil

“…Kim et al. () reported that mozzarella cheese wrapped with a chicken bone gelatin film containing 1% cinnamon bark essential oil demonstrated antimicrobial activity against Listeria monocytogenes for 20 days. Our results suggest that the SSP/PLA‐laminated films containing CBO can be applied under real food storage conditions.…”

“…The absorbance was measured at 734 nm. FRAP was evaluated (Kim et al., ). The film solution (200 μL) and FRAP solution (3800 μL) were mixed and reacted.…”

“…Although the model system is different from real food storage conditions, these results suggest that CBO could be released from the SSP/PLAlaminated films, and the released amount increased proportionally to the CBO concentration and could be maintained for 1 week. Kim et al (2018) reported that mozzarella cheese wrapped with a chicken bone gelatin film containing 1% cinnamon bark essential oil demonstrated antimicrobial activity against Listeria monocytogenes for 20 days. Our results suggest that the SSP/PLA-laminated films containing CBO can be applied under real food storage conditions.…”

“…In particular, essential oils have desirable effects, even at low concentrations, and can be used in foods (Atarés & Chiralt, 2016). Cinnamon bark essential oil (CBO), which is extracted from the bark of Cinnamon (Cinnamomum verum), contains trans-cinnamaldehyde, cinnamyl acetate, benzaldehyde, limonene, and eugenol (Kim, Beak, Yang, & Song, 2018). Trans-cinnamaldehyde is a key component of CBO and exhibits strong antimicrobial activity against many types of bacteria (Zhang, Ma, Critzer, Davidson, & Zhong, 2015).…”