Application of an Automated Hydrophobic Grid Membrane Filter Interpreter System at a Poultry Abattoir

McNab, W. Bruce; Forsberg, Christine M.; Clarke, Robert C.

doi:10.4315/0362-028x-54.8.619

Cited by 13 publications

(4 citation statements)

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“…The correlation coefficient ( r = 0.83) was similar to that obtained with the ATP bioluminescence assay. McNab and coworkers, in a series of papers (McNab et al 1991 crl. 1993) described the use of the HGMF system for determining bacterial load at poultry abattoirs.…”

Section: Comparison Of Hgmf With Plate Countmentioning

confidence: 99%

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Comparison of Microbiological Methods for Monitoring Chicken Carcass Quality

Bautista

Clarke²,

Griffiths

1994

Rapid Methods Automation Mic

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Many methods have been developed to determine microbial levels in food products and these include ATP bioluminescence, hydrophobic grid membrane filtration (HGMF), impediometry and turbidimetry. A comparison of these techniques for detecting microbial levels in chicken carcass rinses was conducted. Only the ATP bioluminescence assay and the HGMF system showed a good correlation with plate counts (r = 0.82 and r = 0.83, respectively). The repeatability of these methods was acceptable. There was also a significant correlation between results obtained with two turbidimetric methods and HGMF as well as between HGMF and ATP bioluminescence data. However, only the ATP bioluminescence assay was able to provide results of microbial levels on a realtime basis (within 10 min). This would be beneficial for HACCP (Hazard Analysis of Critical Control Points) based programs.

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Section: Comparison Of Hgmf With Plate Countmentioning

confidence: 99%

“…Previous work has shown that ATP bioluminescence (Bautista er al. 1994) and hydrophobic grid membrane filtration (HGMF) (McNab et al 1991) can be used to monitor the microbial load on chicken carcasses.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Microbiological Methods for Monitoring Chicken Carcass Quality

Bautista

Clarke²,

Griffiths

1994

Rapid Methods Automation Mic

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“…Poultry carcasses are sampled for microbiological analysis in a number of ways, including tissue collection, carcass skin swab, direct agar contact and whole carcass rinse methods (D’Aoust et al. 1982; McNab et al. 1991; Simmons et al.…”

Section: Introductionmentioning

confidence: 99%

Enumeration of Salmonella from poultry carcass rinses via direct plating methods*

Brichta-Harhay

Arthur

Koohmaraie

2007

Letters in Applied Microbiology

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Aim: To evaluate direct plating methods for the estimation of Salmonella load in poultry carcass rinses. Methods and Results: Two direct plating tools, the spiral plate count method (SPCM) and the hydrophobic grid membrane filtration (HGMF) method, were adapted to support quantification of Salmonella during poultry processing. Test samples consisted of 180 broiler carcasses from a commercial abattoir, 60 from each of three points in the processing line [pre‐inside–outside bird wash (pre‐IOBW), prechill and postchill]. The SPCM was used to estimate Salmonella load in pre‐IOBW rinses, while HGMF was used to estimate Salmonella levels in prechill and postchill rinses. Carcass rinses were also evaluated for Salmonella prevalence by enrichment methods. Mean prevalences of Salmonella were 95%, 100% and 41·7%, and the geometric mean loads were 3·7 × 101, 5·6 × 100 and 5·0 × 10−2 CFU ml−1 for pre‐IOBW, prechill and postchill rinses, respectively. Conclusions: The methods described are useful for estimating the concentration of viable and typical Salmonella in poultry carcass rinses. Significance and Impact of the Study: Direct plating enumeration methods can facilitate the monitoring of Salmonella load on poultry carcasses throughout the production process, and the evaluation of new processing intervention strategies.

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“…After 15 min on the bench the HGMF can be counted. In some situations incubation on a TTC-containing agar is satisfactory (McNab et al 1991).…”

Section: Some Established Hgmf Techniquesmentioning

confidence: 99%

Microbiological Analysis of Foods Using Methods Based on Membrane Filtration

Sharpe

1995

Rapid Methods Automation Mic

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SCOPEThis review covers the use of membrane filters (MFs) as filters but not their widespread use as blotting bases for DNA and immunological separations. More detailed reviews of the applications of MFs will be found in Sharpe andPeterkin (1988), andin Sharpe (1994). BRIEF HISTORY OF MEMBRANE FILTERSMembrane filters (nitrocellulose) were used in dialysis experiments as early as 1855 (Fick), before agar was adopted by microbiologists. The first conirner-cia1 production was in Germany. Before World War I1 MFs were used primarily for sterilization, but as laboratories were devastated and water supplies contaminated by bombing raids, they were applied for determining water safety by German hygiene institutes. After the war, the first MF methods for culturing organisms were published (Muller 1947a,b). Until 1963, MFs were made from nitrocellulose or cellulose esters. Today they are available in nylon, polyvinyl chloride, polysulfone, polycarbonate, polyester, and even metals and their oxides. MFs prepared by deposition from solvent have tortuous structures, with pore lengthlwidth ratios of 750 or more (Presswood 1981). Newer polycarbonate and polyester MFs (in which straight cylindrical pores are produced by etching the tracks of nuclear particles) are extremely thin (10 pm), are rendered invisible by the proper mountant, and have permitted development of sensitive techniques based on direct microscopic observation of microorganisms.

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Application of an Automated Hydrophobic Grid Membrane Filter Interpreter System at a Poultry Abattoir

Cited by 13 publications

References 0 publications

Comparison of Microbiological Methods for Monitoring Chicken Carcass Quality

Comparison of Microbiological Methods for Monitoring Chicken Carcass Quality

Enumeration of Salmonella from poultry carcass rinses via direct plating methods*

Microbiological Analysis of Foods Using Methods Based on Membrane Filtration

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