Application of an automated immunomagnetic separation-enzyme immunoassay for the detection of Salmonella spp. during an outbreak associated with a retail premises

Duncanson, P.; Wareing, D. R. A.; Jones, Owen R.

doi:10.1046/j.1472-765x.2003.01365.x

Cited by 11 publications

(6 citation statements)

References 12 publications

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“…Many papers have been published concerning unsatisfactory hygienic practices of food-handlers (Haupt, Köfer, & Fuchs, 1999;Henroid & Sneed, 2004;Uppman & Reuter, 1998) and outbreaks caused by them (Duncanson, Wareing, & Jones, 2003;Kishimoto et al, 2004;LaPorte et al, 2003). According to our knowledge, this is the Wrst reported study dealing with hygienic working practices of maintenance personnel in the food industry.…”

Section: Discussionmentioning

confidence: 97%

The hygienic working practices of maintenance personnel and equipment hygiene in the Finnish food industry

Aarnisalo

Tallavaara²,

Wirtanen

et al. 2006

Food Control

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Section: Discussionmentioning

confidence: 97%

The hygienic working practices of maintenance personnel and equipment hygiene in the Finnish food industry

Aarnisalo

Tallavaara²,

Wirtanen

et al. 2006

Food Control

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“…Immunomagnetic separation (IMS) has been shown to be a sensitive method for the detection of Salmonella in food samples (Cudjoe et al 1994(Cudjoe et al , 1995Cudjoe and Krona 1997) and automation of IMS (AIMS) has been applied for investigation into a food-borne disease outbreak of Salmonella (Duncanson et al 2003). The Most Probable Number (MPN) procedure has been used for estimating the numbers of Salmonella on poultry carcases and rinses (Jetton et al 1992;Whittemore 1993;Humbert et al 1997) and a combination of MPN and IMS has been used for the enumeration of E. coli O157 in meats (Chapman et al 2001) and in cattle faeces (Fegan et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Quantification and prevalence of Salmonella in beef cattle presenting at slaughter

et al. 2004

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Methods and Results: Automated immunomagnetic separation (AIMS) was used for detection and isolation of Salmonella enriched from cattle faeces. Salmonella were enumerated from positive samples using a combination of the Most Probable Number (MPN) technique and AIMS. A total of 310 faecal samples were tested, 155 were from lot-fed cattle and 155 from grass-fed cattle. Salmonella spp. were isolated from 21 (6AE8%) of the cattle and the prevalence amongst grass-fed cattle (4AE5%) was not significantly different to that found in lot-fed cattle (9%). Counts of Salmonella in positive faeces varied from <3 MPN g)1 of faeces to 2AE8 · 10 3 MPN g )1 and 71% of positive samples had counts <10 MPN g )1 faeces. There was no significant difference in the mean log 10 number of Salmonella in faeces of cattle from each production system. Conclusion: Low numbers of beef cattle were found to shed Salmonella at the time of slaughter and the prevalence and the associated faecal concentrations did not vary significantly with the pre-slaughter production system (grass or lot feeding). The faecal concentration of Salmonella in the majority of faeces was low (<10 MPN g )1 ) with few high concentrations up to 3 · 10 3 MPN g )1 , suggesting there may be a low risk of carcase contamination.Significance and Impact of the Study: Beef cattle do not appear to be a major source of entry of Salmonella into the human food chain and the quantitative information contained in this study can be used in quantitative assessments of the associated risk of human salmonellosis.

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“…Initially, 66 outbreaks were considered eligible for inclusion . Upon closer scrutiny, one outbreak occurred outside England and Wales [64], in five outbreaks initial foodborne transmission was followed by person-to-person transmission [17,38,52,57,67] three described the outbreak in insufficient detail for analysis [9,69,70], one was an outbreak review [28] and one was a news report [55]. These outbreaks were excluded from further analysis, leaving 55 foodborne general outbreaks identified in the literature (' literature outbreaks ').…”

Section: Resultsmentioning

confidence: 99%

Publication bias in foodborne outbreaks of infectious intestinal disease and its implications for evidence-based food policy. England and Wales 1992–2003

O’Brien

Gillespie²,

Sivanesan³

et al. 2006

Epidemiol. Infect.

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Systematic national surveillance of outbreaks of infectious intestinal disease (IID) was introduced in England and Wales in 1992 to provide comprehensive information on causative organisms, sources or vehicles of infection and modes of transmission. We compared information from this system with that published in the peer-reviewed literature between 1 January 1992 and 31 January 2003 to assess the potential effect of publication bias on food-safety policy. During the study period 1763 foodborne outbreaks of IID were reported to national surveillance. Fifty-five were published in the peer-reviewed literature. The peer-reviewed literature overestimated the impacts of milk/milk products, miscellaneous foods (e.g. sandwiches) and desserts and underestimated those of poultry, fish and shellfish, red meat/meat products and eggs/egg products. Without systematic surveillance, knowledge of causative organisms, sources or vehicles of infection and modes of transmission, as gleaned from the peer-reviewed literature, would potentially distort food-safety policy.

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Application of an automated immunomagnetic separation-enzyme immunoassay for the detection of Salmonella spp. during an outbreak associated with a retail premises

Cited by 11 publications

References 12 publications

The hygienic working practices of maintenance personnel and equipment hygiene in the Finnish food industry

The hygienic working practices of maintenance personnel and equipment hygiene in the Finnish food industry

Quantification and prevalence of Salmonella in beef cattle presenting at slaughter

Publication bias in foodborne outbreaks of infectious intestinal disease and its implications for evidence-based food policy. England and Wales 1992–2003

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