Application of an immuno-enzyme technique to titration of antibodies in leptospirosis: ELISA (Enzyme-Linked Immunosorbent Assay)

Mailloux, M; Mazzonelli, J.; Dufresne, Yoann

doi:10.1016/s0176-6724(84)80073-5

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Serological Diagnosis1

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2001

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“…CF was applied to veterinary diagnosis, but species-specific differences were noted (488). CF tests have generally been replaced by ELISA methods (11,365,440,565,566). IgM antibodies become detectable during the first week of illness (11,112,173,351,617), allowing the diagnosis to be confirmed and treatment initiated while it is likely to be most effective.…”

Section: Serological Diagnosismentioning

confidence: 99%

Leptospirosis

2001

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Leptospirosis is a worldwide zoonotic infection with a much greater incidence in tropical regions and has now been identified as one of the emerging infectious diseases. The epidemiology of leptospirosis has been modified by changes in animal husbandry, climate, and human behavior. Resurgent interest in leptospirosis has resulted from large outbreaks that have received significant publicity. The development of simpler, rapid assays for diagnosis has been based largely on the recognition that early initiation of antibiotic therapy is important in acute disease but also on the need for assays which can be used more widely. In this review, the complex taxonomy of leptospires, previously based on serology and recently modified by a genotypic classification, is discussed, and the clinical and epidemiological value of molecular diagnosis and typing is also evaluated

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Section: Serological Diagnosismentioning

confidence: 99%