Application of an untargeted metabolomics approach for the identification of compounds that may be responsible for observed differential effects in chickens fed an organic and a conventional diet

Abstract

boom. Application of untargeted metabolomics approach for identification of compounds that may be responsible for observed differential effects in chickens fed an organic and a conventional diet. Food Additives and Contaminants, 2012, pp.1. <10.1080/19440049.2011 The aim of this study was to apply an untargeted NMR and LC-MS based metabolomics approach to detect potential differences between an organically and a conventionally produced feed, that caused statistically significant differences in growth, in the response to an immunological challenge and in the gene expression profiles in the small intestine of laying hens. A fractionation procedure was set-up to create multiple fractions of the feed, which were subsequently analyzed by NMR and UPLC-TOF/MS operating in positive mode. Comparison of the profiles revealed that the most apparent differences came from the isoflavones in the soy as well as a compound with a molecular mass of 441.202 (M+1)+, which was identified as N,N'-diferuloylputrescine (DFP) and came from the corn.Whether the observed differences in effects are due to the higher levels of isoflavones and DFP is unclear, as is the fact whether the observed differences are typical for organic or conventional produced corn and soy. However, this study shows that this metabolomics approach is suitable for detecting potential differences between products, even in levels of compounds that would have been overlooked with a more targeted AbstractThe aim of this study was to apply an untargeted NMR and LC-MS based metabolomics approach to detect potential differences between an organically and a conventionally produced feed, that caused statistically significant differences in growth, in the response to an immunological challenge and in the gene expression profiles in the small intestine of laying hens. A fractionation procedure was set-up to create multiple fractions of the feed, which were subsequently analyzed by NMR and UPLC-TOF/MS operating in positive mode. Comparison of the profiles revealed that the most apparent differences came from the isoflavones in the soy as well as a compound with a molecular mass of 441.202 (M+1) + , which was identified as N,N'-diferuloylputrescine (DFP) and came from the corn. Whether the observed differences in effects are due to the higher levels of isoflavones and DFP is unclear, as is the fact whether the observed differences are typical for organic or conventional produced corn and soy. However, this study shows that this metabolomics approach is suitable for detecting potential differences between

Health effects of organic farming : Overview literature since 2005

“…Later RIKILT conducted research in which untargeted as well as targeted were measured. In this study, the substance N, N0-diferuloyl putrescine (DFP) was found in corn, which was different in organic and conventional corn (Ruiz-Aracama et al, 2012). Unfortunately not much was known about that substance, but it could be a research topic for follow-up research.…”

Taste compounds, affecting factors, and methods used to evaluate chicken soup: A review

“…NMR, mainly used to analyze polar metabolites, permits the detection of a broad scope of analytes within a single run. However, NMR typically exhibits disadvantages such as lower sensitivity compared to GC‐MS and LC‐MS (Lei et al., 2011 ; Ruiz‐Aracama et al., 2012 ).…”

Gezondheidseffecten biologische landbouw : Overzicht literatuur sinds 2005

“…Later is door RIKILT nog onderzoek gedaan, waarbij naast targeted ook untargeted is gemeten. Hierbij is er in mais de stof N,N0-diferuloylputrescine (DFP) gevonden die verschillend was in biologisch en gangbaar(Ruiz-Aracama et al, 2012). Van die stof was helaas niet zoveel bekend maar het zou een onderzoeksonderwerp kunnen zijn voor vervolgonderzoek.…”