Application of anti‐ssDNA monoclonal antibody to study exogenous and apoptosis‐associated DNA damage

Frankfurt, Oskar S.; Krishan, Awtar

doi:10.1002/cyto.a.20629

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…A specific assay for apoptosis, Apostain for ssDNA was carried out to determine apoptosis in cells (Frankfurt & Krishan, 2008). Median fluorescence intensities obtained from flow cytometric analysis were plotted as bar graphs for HT29 ( Fig.…”

Section: Ltu28 and Ltu31 Stimulate Apoptosis In Combination With Radimentioning

confidence: 99%

Radiosensitizing activity of novel small molecule BRCA1 and DNA-PK inhibitors in lung and colon carcinoma

Radhamani

Suraj

Meehan-Andrews

et al. 2017

Journal of Radiation Research and Applied Sciences

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The DNA dependant protein kinase (DNA-PK) enzyme plays a major part in the repair of double stranded breaks induced by radiation and hence in the radio-resistance of tumour cells. Inhibitors of DNA-PK have been tested successfully in the past for their ability to sensitize cancer cells to the effects of radiation. Here we present two novel benzoxazines (LTU28 and LTU31) and analyse their ability to cause sensitization of cancer cells to radiation. There was a significant reduction in survival rate, increase in apoptosis and inhibition in autophosphorylation of DNA-PK and AKT1 after treating them with LTU28 concomitantly with radiation. The mechanism of action by LTU28 appears to be through inhibition of DNA-PK leading to delayed DNA repair and promotion of apoptosis. LTU31 showed an inhibition in the phosphorylation of BRCA1, thereby blocking DNA repair by homologous recombination.

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Section: Ltu28 and Ltu31 Stimulate Apoptosis In Combination With Radimentioning

confidence: 99%

Radiosensitizing activity of novel small molecule BRCA1 and DNA-PK inhibitors in lung and colon carcinoma

Radhamani

Suraj

Meehan-Andrews

et al. 2017

Journal of Radiation Research and Applied Sciences

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High dietary taurine inhibits myocardial apoptosis during an atherogenic diet: association with increased myocardial HSP70 and HSF-1 but not caspase 3

et al. 2013

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Radiosensitizing activity of a novel Benzoxazine through the promotion of apoptosis and inhibition of DNA repair

Radhamani¹,

Bradley²,

Meehan-Andrews³

et al. 2014

Invest New Drugs

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The DNA dependant protein kinase (DNA-PK) enzyme plays a major part in the repair of double stranded breaks induced by radiation and hence in the radio-resistance of tumour cells. Inhibitors of DNA-PK have been tested successfully in the past for their ability to sensitize cancer cells to the effects of radiation. Here we present a novel benzoxazine, 8-methyl-2-(morpholine-4yl)-7-(pyridine-3-methoxy)-4H-1,3-benzoxacine-4-one (LTU27) and analyse its ability to cause sensitization of lung cancer and colon cancer cells to radiation. There was a significant reduction in survival rate, increase in apoptosis and inhibition in autophosphorylation of DNA-PK and AKT1 after treating them concomitantly with both radiation and LTU27. The mechanism of action appears to be through inhibition of DNA-PK leading to delayed DNA repair and promotion of apoptosis.

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Application of anti‐ssDNA monoclonal antibody to study exogenous and apoptosis‐associated DNA damage

Cited by 3 publications

References 13 publications

Radiosensitizing activity of novel small molecule BRCA1 and DNA-PK inhibitors in lung and colon carcinoma

Radiosensitizing activity of novel small molecule BRCA1 and DNA-PK inhibitors in lung and colon carcinoma

High dietary taurine inhibits myocardial apoptosis during an atherogenic diet: association with increased myocardial HSP70 and HSF-1 but not caspase 3

Radiosensitizing activity of a novel Benzoxazine through the promotion of apoptosis and inhibition of DNA repair

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