Application of Antibody and Immunoassay for Food Safety

Lei, Hongtao; Wang, Zhanhui; Eremin, Sergei A.; Liu, Zhiwei

doi:10.3390/foods11060826

Cited by 8 publications

(1 citation statement)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, a number of scientists have proposed that scFv can replace traditional animal-derived antibodies; however, others believe that the preparation and application technology of scFv is not mature enough, F I G U R E 1 Basic structure of typical immunoglobulin G (IgG) antibody and scFv antibody and scFv should be used as a supplementary immune reagent, and not as a substitute (Peltomaa et al, 2022). Currently, scFvs are widely used in clinical treatment; however, their application in the field of food safety must be strengthened (Lei et al, 2022). It is generally accepted that phage display is still the first choice for the preparation of scFv (Hairul Bahara et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Single‐chain fragment variable produced by phage display technology: Construction, selection, mutation, expression, and recent applications in food safety

et al. 2022

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

Immunoassays are reliable, efficient, and accurate methods for the analysis of small‐molecule harmful substances (such as pesticides, veterinary drugs, and biological toxins) that may be present in food. However, traditional polyclonal and monoclonal antibodies are limited by animal hosts and hinder further development of immunoassays. With the gradual application of phage display technology as an efficient in vitro selection technology, the single‐chain fragment variable (scFv) now provides an exciting alternative to traditional antibodies. Efficiently constructed scFv source libraries and specifically designed biopanning schemes can now yield scFvs possessing specific recognition capabilities. A rational mutation strategy further enhances the affinity of scFv, and allows it to reach a level that cannot be achieved by immunization. Finally, appropriate prokaryotic expression measures ensure stable and efficient production of scFv. Therefore, when developing excellent scFvs, it is necessary to focus on three key aspects of this process that include screening, mutation, and expression. In this review, we analyze in detail the preparation and affinity improvement process for scFv and provide insights into the research progress and development trend of scFv‐based immunoassay methods for monitoring small‐molecule harmful substances.

show abstract

Section: Introductionmentioning

confidence: 99%